{
    "abstract": "Apurinic/apyrimidinic endonuclease 1 (APE1) has been considered as a promising biomarker since it is associated with numerous human diseases, involving neurodegenerative diseases and cancer. However, current APE1 detection methods mainly rely on immunology-based methods, which are burdened by time-consuming and procedural complexity. To overcome these shortcomings, we have developed an innovative all-in-one technique that simplifies APE1 detection by integrating enzyme-responsive elements structure-switchable dsDNA promoter with CRISPR/AsCas12a methodology, namely EDC. In this work, the structure-switchable dsDNA promoter has been well-designed to trigger the site-directed incision of APE1 and then release the split activator to illumine the CRISPR/AsCas12a catalyst system by coupling it with another truncated activator. Under optimal circumstances, the proposed strategy enables sensitive detection of the target APE1 with a detection limit of 4.8\u00a0\u00d7\u00a010",
    "authors": [
        {
            "affiliation": "Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China.",
            "firstname": "Xingrong",
            "initials": "X",
            "lastname": "Li"
        },
        {
            "affiliation": "Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China.",
            "firstname": "Yumei",
            "initials": "Y",
            "lastname": "Li"
        },
        {
            "affiliation": "Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China.",
            "firstname": "Cuixiang",
            "initials": "C",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China.",
            "firstname": "Jiatong",
            "initials": "J",
            "lastname": "Chai"
        },
        {
            "affiliation": "Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China.",
            "firstname": "Hongmao",
            "initials": "H",
            "lastname": "Liu"
        },
        {
            "affiliation": "Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China.",
            "firstname": "Xinli",
            "initials": "X",
            "lastname": "Jiang"
        },
        {
            "affiliation": "Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, People's Republic of China; Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan, People's Republic of China; Hubei Engineering Center for Infectious Disease Prevention, Control and Treatment, Wuhan, People's Republic of China; Hubei Provincial Clinical Medical Research Center for Molecular Diagnostics, Wuhan, People's Republic of China. Electronic address: liyirong838@163.com.",
            "firstname": "Yirong",
            "initials": "Y",
            "lastname": "Li"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2025 Elsevier B.V. All rights reserved.",
    "doi": "10.1016/j.talanta.2025.128161",
    "journal": "Talanta",
    "keywords": [
        "APE1",
        "CRISPR/AsCas12a",
        "Molecular diagnostics",
        "Site-directed incision",
        "Structure-switchable"
    ],
    "methods": null,
    "publication_date": "2025-04-23",
    "pubmed_id": "40262340",
    "results": null,
    "title": "Structure-switchable dsDNA promoter regulates the activity of CRISPR-Cas12a for APE1 detection.",
    "xml": "<Element 'PubmedArticle' at 0x7885034c0fe0>"
}