{
    "abstract": "The increase in multidrug resistance among colon cancer cells presents a challenge for the development of effective therapies. Small-molecule analogues of second mitochondria-derived activator of caspase (SMAC) mimetic in association with mixed lineage kinase domain-like protein (MLKL)-pDNA and z-VAD-fmk have shown ideal antitumor effects in colon cancer cells in vitro via induction of RIP3-dependent necroptosis. To achieve synergistic antitumor effects in vivo, liposomes loaded with SMAC mimetic, MLKL-pDNA and z-VAD-fmk have been developed using novel lipid fusion methods to co-localise the molecules of interest within the tumour cells. The co-encapsulation liposome (MLKL-zVAD-BV6-LP) had a high entrapment efficiency of approximately 95% for both zVAD and BV6 and was able to condense MLKL-pDNA very well. The vectors showed good biocompatibility, tumour targeting and small-molecule co-localisation. In a CT26 mouse model, the MLKL-zVAD-BV6-LP exhibited a tumour-suppression rate of over 60% in vivo, which was significantly higher than that of both the null-liposome and coadministration groups. Above all, the co-encapsulation system provided a novel approach to combination tumour therapy.",
    "authors": [
        {
            "affiliation": "a College of Life Sciences , Sichuan University , Chengdu , Sichuan , PR China.\nb State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy , West China Hospital, Sichuan University , Chengdu , Sichuan , PR China.",
            "firstname": "Dan",
            "initials": "D",
            "lastname": "Sun"
        },
        {
            "affiliation": "c Division of Biosciences, Faculty of Life Sciences , University College London , London , UK.",
            "firstname": "Linshu",
            "initials": "L",
            "lastname": "Zhao"
        },
        {
            "affiliation": "d Department of Colorectal Surgery , Sun Yat-sen University Cancer Center , Guangzhou , China.",
            "firstname": "Junzhong",
            "initials": "J",
            "lastname": "Lin"
        },
        {
            "affiliation": "a College of Life Sciences , Sichuan University , Chengdu , Sichuan , PR China.",
            "firstname": "Yun",
            "initials": "Y",
            "lastname": "Zhao"
        },
        {
            "affiliation": "b State Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy , West China Hospital, Sichuan University , Chengdu , Sichuan , PR China.",
            "firstname": "Yu",
            "initials": "Y",
            "lastname": "Zheng"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.1080/1061186X.2017.1339192",
    "journal": "Journal of drug targeting",
    "keywords": [
        "MLKL-pDNA",
        "SMAC mimetic",
        "co-encapsulation liposome",
        "liposome fusion",
        "zVAD"
    ],
    "methods": null,
    "publication_date": "2017-06-27",
    "pubmed_id": "28649853",
    "results": null,
    "title": "Cationic liposome co-encapsulation of SMAC mimetic and zVAD using a novel lipid bilayer fusion loaded with MLKL-pDNA for tumour inhibition in vivo.",
    "xml": "<Element 'PubmedArticle' at 0x78850309c720>"
}