{
    "abstract": "Osteochondral defect regeneration is challenging due to the mismatch between cartilage and subchondral bone. We developed a functionalized scaffold replicating the natural architecture, biochemical and biomechanical environment of both tissues to promote concurrent regeneration. Our bilayered, zone-specific scaffold combines tailored materials for each tissue type: gelatin methacryloyl (GelMA), modified hyaluronic acid, and umbilical cord-derived extracellular matrix (ECM) for the cartilage layer; GelMA, placenta-derived ECM, and nano amorphous calcium phosphate for the osseous layer. Using 3D digital light-processing printing, we constructed the scaffold with spatially distributed biochemical and biomechanical signaling. This approach created dual chondro-/osteogenic microenvironments facilitating bone marrow mesenchymal stem cell differentiation. ",
    "authors": [
        {
            "affiliation": "Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX12JD, U.K.",
            "firstname": "Xinyi",
            "initials": "X",
            "lastname": "Ouyang"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Rui",
            "initials": "R",
            "lastname": "Li"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Wei",
            "initials": "W",
            "lastname": "Sun"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Yuqing",
            "initials": "Y",
            "lastname": "Gu"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Junxin",
            "initials": "J",
            "lastname": "Lin"
        },
        {
            "affiliation": "Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.",
            "firstname": "Zhang",
            "initials": "Z",
            "lastname": "Fan"
        },
        {
            "affiliation": "International Institutes of Medicine, The fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China.",
            "firstname": "Xudong",
            "initials": "X",
            "lastname": "Yao"
        },
        {
            "affiliation": "Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.",
            "firstname": "Hongyi",
            "initials": "H",
            "lastname": "Gu"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Chang",
            "initials": "C",
            "lastname": "Xie"
        },
        {
            "affiliation": "Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Wenyue",
            "initials": "W",
            "lastname": "Li"
        },
        {
            "affiliation": "Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Yifei",
            "initials": "Y",
            "lastname": "Yang"
        },
        {
            "affiliation": "Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Yiyang",
            "initials": "Y",
            "lastname": "Yan"
        },
        {
            "affiliation": "International Institutes of Medicine, The fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China.",
            "firstname": "Wei",
            "initials": "W",
            "lastname": "Wei"
        },
        {
            "affiliation": "International Institutes of Medicine, The fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China.",
            "firstname": "Bingbing",
            "initials": "B",
            "lastname": "Wu"
        },
        {
            "affiliation": "International Institutes of Medicine, The fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China.",
            "firstname": "Xiuying",
            "initials": "X",
            "lastname": "Chen"
        },
        {
            "affiliation": "Department of Orthopedic Surgery, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China.\nDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.",
            "firstname": "Bin",
            "initials": "B",
            "lastname": "He"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Shufang",
            "initials": "S",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Yi",
            "initials": "Y",
            "lastname": "Hong"
        },
        {
            "affiliation": "Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX12JD, U.K.\nOxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou Industrial Park, Jiangsu 215123, China.",
            "firstname": "Zhanfeng",
            "initials": "Z",
            "lastname": "Cui"
        },
        {
            "affiliation": "Department of Sports Medicine of the Second Affiliated Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nDr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China.\nZhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Haining, Zhejiang 314400, China.\nChina Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, Zhejiang 310058, China.",
            "firstname": "Xiaozhao",
            "initials": "X",
            "lastname": "Wang"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.1021/acsami.4c14063",
    "journal": "ACS applied materials & interfaces",
    "keywords": [
        "DLP 3D printing",
        "bilineage differentiation",
        "biochemical cues",
        "matrix stiffness",
        "osteochondral repair",
        "tissue-derived ECM"
    ],
    "methods": null,
    "publication_date": "2025-03-31",
    "pubmed_id": "40162492",
    "results": null,
    "title": "3D-Printed Dual-Lineage Inductive Approach for Efficient Osteochondral Regeneration.",
    "xml": "<Element 'PubmedArticle' at 0x788504365170>"
}