{
    "abstract": "RNA interference (RNAi) has emerged as a promising and environmentally friendly approach for controlling the pest Henosepilachna vigintioctopunctata (Hvig). Identifying lethal target genes in Hvig and evaluating the efficacy of oral dsRNA administration are crucial steps in this process. Additionally, assessing the potential risks of RNAi to non-target organisms (NTOs) is essential to ensure environmental safety. A soluble N-ethylmaleimide-sensitive factor attachment protein \u03b1 (\u03b1SNAP) is an essential component of membrane fusion machinery, offering as a potential target gene for RNAi-based pest control. This study found that silencing of Hv\u03b1snap with varying dsRNA concentrations (6.25, 12.5, 25, 50, 100, 200\u00a0ng/\u03bcL) induced 53.33\u00a0%-100\u00a0% lethality in Hvig, with an LC",
    "authors": [
        {
            "affiliation": "State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.",
            "firstname": "Zhaoyang",
            "initials": "Z",
            "lastname": "Li"
        },
        {
            "affiliation": "State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.",
            "firstname": "Junna",
            "initials": "J",
            "lastname": "Liu"
        },
        {
            "affiliation": "School of Biotechnology, Centurion University of Technology and Management, Bhubaneswar 752050, India.",
            "firstname": "Satyabrata",
            "initials": "S",
            "lastname": "Nanda"
        },
        {
            "affiliation": "State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.",
            "firstname": "Zexin",
            "initials": "Z",
            "lastname": "Zhong"
        },
        {
            "affiliation": "State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.",
            "firstname": "Xuming",
            "initials": "X",
            "lastname": "Luo"
        },
        {
            "affiliation": "Department of Entomology, University of Illinois Urbana-Champaign, Urbana 61801-3795, USA.",
            "firstname": "Xuguo",
            "initials": "X",
            "lastname": "Zhou"
        },
        {
            "affiliation": "State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.",
            "firstname": "Youjun",
            "initials": "Y",
            "lastname": "Zhang"
        },
        {
            "affiliation": "State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol, Ministry of Education, South China Agricultural University, Guangzhou 510642, China. Electronic address: yangchunxiao@scau.edu.cn.",
            "firstname": "Chunxiao",
            "initials": "C",
            "lastname": "Yang"
        },
        {
            "affiliation": "State Key Laboratory of Green Pesticide, Engineering Research Center of Biocontrol, Ministry of Education, South China Agricultural University, Guangzhou 510642, China. Electronic address: panhuipeng@scau.edu.cn.",
            "firstname": "Huipeng",
            "initials": "H",
            "lastname": "Pan"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2025 Elsevier Inc. All rights reserved.",
    "doi": "10.1016/j.pestbp.2025.106381",
    "journal": "Pesticide biochemistry and physiology",
    "keywords": [
        "Gene silencing",
        "Henosepilachna vigintioctopunctata",
        "Non-target effects",
        "Propylaea japonica",
        "RNA interference"
    ],
    "methods": null,
    "publication_date": "2025-04-23",
    "pubmed_id": "40262866",
    "results": null,
    "title": "RNAi effect in target and non-target pests correlates with the length of continuous matches in dsRNA sequences.",
    "xml": "<Element 'PubmedArticle' at 0x7885034c8a40>"
}