{
    "abstract": "This study aimed to investigate the effects of curcumin (Cur) on programmed cell death 1 ligand 1 (PD-L1) expression in neutrophils from septic rats and its regulatory influence on T-lymphocyte apoptosis and lung injury in a rat sepsis model.\nCecum ligation and puncture (CLP) experiments were conducted to establish a rat sepsis model, with the subsequent grouping of rats based on curcumin administration. Rats were monitored for 7 days to assess the 7-day survival rate. Serum, lung tissues, and thymus tissues were collected. Flow cytometry and immunohistochemistry were utilized to assess the number of PD-L1-positive neutrophils and PD-L1 positivity in both blood and lung tissues. Hematoxylin and eosin (HE) staining and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) histochemistry were employed to examine pathological changes and cell apoptosis in lung and thymus tissues. Furthermore, a kit was employed to measure the activity of myeloperoxidase (MPO), a marker of neutrophil activation, in lung tissues. Enzyme-linked immunosorbent assay (ELISA) was utilized to determine plasma levels of inflammatory factors. Neutrophils were extracted and co-cultured with normal T lymphocytes. TUNEL assays were used to evaluate T-lymphocyte apoptosis, and Western blotting was performed to analyze the expression of PD-L1 and programmed cell death 1 (PD-1).\nIn \nCurcumin effectively mitigates lung and thymus injury during sepsis and attenuates the apoptosis of rat T lymphocytes by down-regulating PD-L1 expression in centrocytes, both ",
    "authors": [
        {
            "affiliation": "Department of Emergency Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 200437 Shanghai, China.",
            "firstname": "Jiajun",
            "initials": "J",
            "lastname": "Zou"
        },
        {
            "affiliation": "Department of Emergency Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 200437 Shanghai, China.",
            "firstname": "Jian",
            "initials": "J",
            "lastname": "Guo"
        },
        {
            "affiliation": "Department of Emergency Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 200437 Shanghai, China.",
            "firstname": "Guanyu",
            "initials": "G",
            "lastname": "Hu"
        },
        {
            "affiliation": "Department of Emergency Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 200437 Shanghai, China.",
            "firstname": "Yiming",
            "initials": "Y",
            "lastname": "Qian"
        }
    ],
    "conclusions": "Curcumin effectively mitigates lung and thymus injury during sepsis and attenuates the apoptosis of rat T lymphocytes by down-regulating PD-L1 expression in centrocytes, both ",
    "copyrights": null,
    "doi": "10.24976/Discov.Med.202537195.67",
    "journal": "Discovery medicine",
    "keywords": [
        "PD-L1",
        "T-lymphocyte apoptosis",
        "cecum ligation and puncture",
        "curcumin",
        "lung injury",
        "neutrophil",
        "sepsis"
    ],
    "methods": null,
    "publication_date": "2025-04-27",
    "pubmed_id": "40287812",
    "results": "In ",
    "title": "Curcumin Attenuates PD-L1-Positive Neutrophil-Induced T-Lymphocyte Apoptosis and Alleviates Lung Injury During Sepsis in Rats.",
    "xml": "<Element 'PubmedArticle' at 0x788502f5aed0>"
}