{
    "abstract": "Fibrinogen concentration is a major determinant of both clotting and bleeding risk. Clotting and bleeding disorders cause extensive morbidity and mortality, particularly in resource-poor and emergency settings. This is exacerbated by a lack of timely intervention informed by measurement of fibrinogen levels under conditions such as thrombosis or postpartum haemorrhage. There is an absence of simple, rapid, low-cost, and sustainable diagnostic devices for fibrinogen measurement that can be deployed in such environments. Paper-based analytical devices are of significant interest due to their potential for low-cost production, ease of use, and environmental sustainability. In this work, a device for measuring blood plasma fibrinogen using chromatography paper was developed. Wax printing was used to create hydrophobic structures to define the test channel and sample application zone. Test strips were modified with bovine thrombin. Plasma samples (22 \u03bcL) were applied, and the flow rate was monitored over 5 min. As the sample traversed the strip, clotting was induced by the conversion of soluble fibrinogen to insoluble fibrin. The flow rate and distance travelled by the sample were dependent on fibrinogen concentration. The device was able to measure fibrinogen concentration in the range of 0.5-7.0 \u00b1 0.3 mg/mL (",
    "authors": [
        {
            "affiliation": "Centre for Research in Biosciences (CRIB), Department of Applied Sciences, University of the West of England, Coldhar-bour Lane, Bristol BS16 1QY, U.K.",
            "firstname": "Jerro",
            "initials": "J",
            "lastname": "Saidykhan"
        },
        {
            "affiliation": "Centre for Research in Biosciences (CRIB), Department of Applied Sciences, University of the West of England, Coldhar-bour Lane, Bristol BS16 1QY, U.K.",
            "firstname": "Laura",
            "initials": "L",
            "lastname": "Selevic"
        },
        {
            "affiliation": "Department of Pharmacy, University of Naples \"Federico II\", Via Domenico Montesano 49, Napoli 80131, Italy.",
            "firstname": "Stefano",
            "initials": "S",
            "lastname": "Cinti"
        },
        {
            "affiliation": "Centre for Research in Biosciences (CRIB), Department of Applied Sciences, University of the West of England, Coldhar-bour Lane, Bristol BS16 1QY, U.K.",
            "firstname": "Jennifer E",
            "initials": "JE",
            "lastname": "May"
        },
        {
            "affiliation": "Centre for Research in Biosciences (CRIB), Department of Applied Sciences, University of the West of England, Coldhar-bour Lane, Bristol BS16 1QY, U.K.",
            "firstname": "Anthony J",
            "initials": "AJ",
            "lastname": "Killard"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.1021/acs.analchem.1c03665\n10.1002/ajh.21293\n10.1093/bja/aes096\n10.1021/ac902763a\n10.1055/s-0037-1613124\n10.1111/bjh.15524\n10.1213/ANE.0000000000000448\n10.1002/adhm.201900244\n10.1042/EBC20150012\n10.1016/j.bios.2020.112715\n10.1016/j.talanta.2018.10.104\n10.1016/j.aca.2017.11.010\n10.1021/acs.analchem.8b03636\n10.1039/c9an00616h\n10.1021/acssensors.0c01937\n10.1016/j.aca.2019.12.046\n10.1039/d0lc00439a\n10.1021/ac9013989\n10.1055/s-0038-1646807\n10.3390/app7080863\n10.3324/haematol.2009.017251\n10.1373/49.6.868\n10.2147/JBM.S90693\n10.1182/blood-2010-11-316885\n10.21037/jlpm.2018.07.10",
    "journal": "Analytical chemistry",
    "keywords": [],
    "methods": null,
    "publication_date": "2021-10-08",
    "pubmed_id": "34615344\n18951466\n22490316\n20131873\n12083508\n30073664\n25225891\n31081270\n27365041\n33099241\n30609613\n29291790\n30257554\n31294736\n33095567\n32043998\n32588856\n20000334\n462421\n18503132\n20207842\n12765981\n27713652\n21355090",
    "results": null,
    "title": "Paper-Based Lateral Flow Device for the Sustainable Measurement of Human Plasma Fibrinogen in Low-Resource Settings.",
    "xml": "<Element 'PubmedArticle' at 0x788503f0b510>"
}