{
    "abstract": "In this study, we aim to investigate the role of tanshinone IIA in myocardial infarction (MI), especially in left ventricular remodelling (VR) and the underlying mechanism involving the TLR4/MyD88/NF-\u03baB signalling pathway. Sprague-Dawley (SD) rats (n = 96) were selected, and 12 of them underwent sham surgery. The remaining 84 rats were subjected to MI modelling. HE and MT staining were carried out to estimate infract size, histopathological changes and fibrosis degree. Macrophage infiltration and cardiomyocyte apoptosis were evaluated by immunohistochemistry and TUNEL staining. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting were used to determine the expression levels of TLR4, MyD88 and NF-\u03baB. Serum levels of IL-2, IL-6, IL-8, TNF-a, procollagen I Cpropeptide (PICP), and procollagen III N-propeptide (PIIINP) were measured using enzyme-linked immunosorbent assay (ELISA). The heart weight/body weight, mean arterial pressure (MAP), left ventricular end-systolic pressure (LVESP), +dP/dt and -dP/dt increased while the ventricular function and the left ventricular end-diastole pressure (LVEDP) decreased in MI rats. Compared with the rats undergoing sham surgery, MI rats showed larger infarct size, severer fibrosis, higher expression levels of TLR4, NF-\u03baB-P65, MyD88, IL-2, IL-6, IL-8, TNF-a, PICP and PIIINP as well as enhanced macrophage infiltration, cardiomyocyte apoptosis. After treatment with tanshinone IIA combined with LPS for 4 weeks, the rats showed better condition than those treated with only LPS. These results indicate that tanshinone IIA attenuates MI and prevents left VR. Importantly, inhibition of TLR4/MyD88/NF-\u03baB signalling pathway is a key step in this process.",
    "authors": [
        {
            "affiliation": "Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu Province, China.\nCollege of Health Sciences, Jiangsu Normal University, Xuzhou, China.",
            "firstname": "Dong-Mei",
            "initials": "DM",
            "lastname": "Wu"
        },
        {
            "affiliation": "Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu Province, China.\nCollege of Health Sciences, Jiangsu Normal University, Xuzhou, China.",
            "firstname": "Yong-Jian",
            "initials": "YJ",
            "lastname": "Wang"
        },
        {
            "affiliation": "Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu Province, China.\nCollege of Health Sciences, Jiangsu Normal University, Xuzhou, China.",
            "firstname": "Xin-Rui",
            "initials": "XR",
            "lastname": "Han"
        },
        {
            "affiliation": "Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu Province, China.\nCollege of Health Sciences, Jiangsu Normal University, Xuzhou, China.",
            "firstname": "Xin",
            "initials": "X",
            "lastname": "Wen"
        },
        {
            "affiliation": "Department of Cardiovasculare, The Third Hospital of Hebei Medical University, Hebei, China.",
            "firstname": "Lei",
            "initials": "L",
            "lastname": "Li"
        },
        {
            "affiliation": "Department of Cardiovasculare, The Third Hospital of Hebei Medical University, Hebei, China.",
            "firstname": "Lan",
            "initials": "L",
            "lastname": "Xu"
        },
        {
            "affiliation": "Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu Province, China.",
            "firstname": "Jun",
            "initials": "J",
            "lastname": "Lu"
        },
        {
            "affiliation": "Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu Province, China.",
            "firstname": "Yuan-Lin",
            "initials": "YL",
            "lastname": "Zheng"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.",
    "doi": "10.1111/jcmm.13557",
    "journal": "Journal of cellular and molecular medicine",
    "keywords": [
        "myeloid differentiation factor 88",
        "myocardial infarction",
        "nuclear factor-kappaB",
        "tanshinone IIA",
        "toll-like receptor 4",
        "ventricular remodelling"
    ],
    "methods": null,
    "publication_date": "2018-03-11",
    "pubmed_id": "29524303\n16407573\n24066201\n22368166\n20623185\n26482376\n23358460\n15464336\n21774934\n24647357\n27222061\n26968890\n21887820\n26742900\n25332099\n23255009\n19530445\n15464336\n11655130\n26439035\n23908332\n20128155\n12077118\n17072321\n17303919\n16797002\n2103331\n24669820\n16166516\n27324127\n25911951\n11257452\n4113827\n9382882\n25120632\n22201804",
    "results": null,
    "title": "Tanshinone IIA prevents left ventricular remodelling via the TLR4/MyD88/NF-\u03baB signalling pathway in rats with myocardial infarction.",
    "xml": "<Element 'PubmedArticle' at 0x788503cd4680>"
}