{
    "abstract": "A comprehensive investigation of ovarian cancer (OC) progression at the single-cell level is crucial for enhancing our understanding of the disease, as well as for the development of better diagnoses and treatments. Here, over half a million single-cell transcriptome data were collected from 84 OC patients across all clinical stages. Through integrative analysis, we identified heterogeneous epithelial-immune-stromal cellular compartments and their interactions in the OC microenvironment. The epithelial cells displayed clinical subtype features with functional variance. A significant increase in distinct T cell subtypes was identified including Tregs and CD8+ exhausted T cells from stage IC2. Additionally, we discovered antigen-presenting cancer-associated fibroblasts (CAFs), with myofibroblastic CAFs (myCAFs) exhibiting enriched extracellular matrix (ECM) functionality linked to tumor progression at stage IC2. Furthermore, the NECTIN2-TIGIT ligand-receptor pair was identified to mediate T cells communicating with epithelial, fibroblast, endothelial, and other cell types. Knock-out of NECTIN2 using CRISPR/Cas9 inhibited ovarian cancer cell (SKOV3) proliferation, and increased T cell proliferation when co-cultured. These findings shed light on the cellular compartments and functional aspects of OC, providing insights into the molecular mechanisms underlying stage IC2 and potential therapeutic strategies for OC.",
    "authors": [
        {
            "affiliation": "College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 10049, China.\nLars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for LifeScience, BGI Research, Qingdao, 266555, China.\nBGI Research, Shenzhen, 518083, China.",
            "firstname": "Chaochao",
            "initials": "C",
            "lastname": "Chai"
        },
        {
            "affiliation": "College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 10049, China.\nLars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for LifeScience, BGI Research, Qingdao, 266555, China.\nBGI Research, Shenzhen, 518083, China.",
            "firstname": "Langchao",
            "initials": "L",
            "lastname": "Liang"
        },
        {
            "affiliation": "Department of Biomedicine, Aarhus University, Aarhus, Denmark.",
            "firstname": "Nanna S",
            "initials": "NS",
            "lastname": "Mikkelsen"
        },
        {
            "affiliation": "Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.",
            "firstname": "Wei",
            "initials": "W",
            "lastname": "Wang"
        },
        {
            "affiliation": "College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 10049, China.\nLars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for LifeScience, BGI Research, Qingdao, 266555, China.",
            "firstname": "Wandong",
            "initials": "W",
            "lastname": "Zhao"
        },
        {
            "affiliation": "College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 10049, China.\nLars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for LifeScience, BGI Research, Qingdao, 266555, China.",
            "firstname": "Chengcheng",
            "initials": "C",
            "lastname": "Sun"
        },
        {
            "affiliation": "Department of Biomedicine, Aarhus University, Aarhus, Denmark.",
            "firstname": "Rasmus O",
            "initials": "RO",
            "lastname": "Bak"
        },
        {
            "affiliation": "Lars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for LifeScience, BGI Research, Qingdao, 266555, China.\nBGI Research, Shenzhen, 518083, China.",
            "firstname": "Hanbo",
            "initials": "H",
            "lastname": "Li"
        },
        {
            "affiliation": "Department of Biomedicine, Aarhus University, Aarhus, Denmark.\nSteno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark.",
            "firstname": "Lin",
            "initials": "L",
            "lastname": "Lin"
        },
        {
            "affiliation": "Lars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for LifeScience, BGI Research, Qingdao, 266555, China. wangfei@biomed.au.dk.\nBGI Research, Shenzhen, 518083, China. wangfei@biomed.au.dk.\nDepartment of Biomedicine, Aarhus University, Aarhus, Denmark. wangfei@biomed.au.dk.",
            "firstname": "Fei",
            "initials": "F",
            "lastname": "Wang"
        },
        {
            "affiliation": "Lars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for LifeScience, BGI Research, Qingdao, 266555, China. alun@biomed.au.dk.\nBGI Research, Shenzhen, 518083, China. alun@biomed.au.dk.\nDepartment of Biomedicine, Aarhus University, Aarhus, Denmark. alun@biomed.au.dk.\nSteno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark. alun@biomed.au.dk.",
            "firstname": "Yonglun",
            "initials": "Y",
            "lastname": "Luo"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2024. The Author(s).",
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    "journal": "Communications biology",
    "keywords": [],
    "methods": null,
    "publication_date": "2024-01-27",
    "pubmed_id": "38278958\n31099893\n35143424\n36633525\n19793610\n28450177\n26707054\n18851871\n35361264\n35196078\n35883004\n35675036\n17255760\n34739872\n35969151\n36543113\n32747365\n35681617\n32572264\n30243508\n35427494\n31570879\n36216928\n20201977\n37284314\n31740819\n33963000\n32405060\n31282856\n30329142\n18084642\n30463850\n19047092\n21441454\n26927206\n12704383\n19461667\n35122040\n31197017\n35029648\n32505533\n30862717\n33603140\n11295035\n23263632\n21331226\n36671408\n34118087\n32269380\n32212317\n25465800\n23758976\n36293219\n34062119\n34557778\n23323831\n28825705\n28569836\n33597522\n34050182\n35320732\n33852846\n32561858\n34238352\n33158814\n34992217\n34428202",
    "results": null,
    "title": "Single-cell transcriptome analysis of epithelial, immune, and stromal signatures and interactions in human ovarian cancer.",
    "xml": "<Element 'PubmedArticle' at 0x78850548da80>"
}