{
    "abstract": "This study aimed to assess the utility of real-time-polymerase chain reaction (PCR) for diagnosing chronic endometritis (CE) by targeting 11 prevalent pathogens and to compare the outcomes with conventional culture-based diagnosis.\nA retrospective analysis was conducted on 500 patients with clinical conditions such as abnormal bleeding, in vitro fertilization failure, recurrent implantation failure, recurrent miscarriage, and recurrent pregnancy loss. The prevalence of 11 key pathogens associated with CE was evaluated in endometrial biopsy samples.\nIn our study, PCR identified 318 cases (63.6%) positive for at least one of the 11 investigated pathogens, while culture-based methods detected 115 cases (23%). Predominant pathogens detected by PCR included \nThe DICE panel proves itself as a swift, precise, and cost-effective diagnostic tool for detecting both culturable and nonculturable endometrial pathogens in CE. Demonstrating superiority, the Molecular method outshines microbial culture, ensuring accurate and sensitive detection of CE-associated pathogens, harmonizing seamlessly with histology and hysteroscopy findings.",
    "authors": [
        {
            "affiliation": "LifeCell International (P) Ltd. (Nandagopal, Rajan N., Padhiar, Abhaya, and Ghambir), Chennai, Tamil Nadu, India.",
            "firstname": "Murugan",
            "initials": "M",
            "lastname": "Nandagopal"
        },
        {
            "affiliation": "LifeCell International (P) Ltd. (Nandagopal, Rajan N., Padhiar, Abhaya, and Ghambir), Chennai, Tamil Nadu, India.",
            "firstname": "Rajesh",
            "initials": "R",
            "lastname": "Rajan N"
        },
        {
            "affiliation": "LifeCell International (P) Ltd. (Nandagopal, Rajan N., Padhiar, Abhaya, and Ghambir), Chennai, Tamil Nadu, India.",
            "firstname": "Chirayu",
            "initials": "C",
            "lastname": "Padhiar"
        },
        {
            "affiliation": "LifeCell International (P) Ltd. (Nandagopal, Rajan N., Padhiar, Abhaya, and Ghambir), Chennai, Tamil Nadu, India.",
            "firstname": "Mayur",
            "initials": "M",
            "lastname": "Abhaya"
        },
        {
            "affiliation": "Dr Bansal's Arogya Hospital & Arogya Maa IVF (Bansal), Thane, Maharashtra, India.",
            "firstname": "Uma",
            "initials": "U",
            "lastname": "Bansal"
        },
        {
            "affiliation": "LifeCell International (P) Ltd. (Nandagopal, Rajan N., Padhiar, Abhaya, and Ghambir), Chennai, Tamil Nadu, India.",
            "firstname": "Prakash",
            "initials": "P",
            "lastname": "Ghambir"
        }
    ],
    "conclusions": "The DICE panel proves itself as a swift, precise, and cost-effective diagnostic tool for detecting both culturable and nonculturable endometrial pathogens in CE. Demonstrating superiority, the Molecular method outshines microbial culture, ensuring accurate and sensitive detection of CE-associated pathogens, harmonizing seamlessly with histology and hysteroscopy findings.",
    "copyrights": "\u00a9 2024 The Authors.",
    "doi": "10.1016/j.xagr.2024.100377\n10.22074/ijfs.2020.5779\n10.1111/j.1600-0897.2011.01051.x\n10.1186/s12958-018-0414-2\n10.1097/CM9.0000000000001275\n10.5858/2004-128-1000-RSIAIT\n10.1016/j.fertnstert.2017.05.034\n10.1016/j.ajog.2018.02.012\n10.1016/j.ajog.2016.08.008\n10.5653/cerm.2016.43.4.185\n10.3390/ijms23010485\n10.5603/GP.a2022.0147\n10.1111/lam.12359\n10.1128/AEM.02802-12\n10.1099/jmm.0.46731-0\n10.1016/j.diagmicrobio.2006.02.013\n10.1128/JCM.42.2.683-692.2004\n10.1111/j.1469-0691.2010.03217.x\n10.1016/j.diagmicrobio.2006.09.006\n10.1016/j.omtm.2020.12.010\n10.1086/423963\n10.1097/AOG.0b013e31825a6bc9\n10.1016/j.ijtb.2020.08.003\n10.18295/squmj.1.2022.003\n10.18203/2320-1770.ijrcog20200333\n10.1186/s12958-017-0233-x\n10.1007/s10815-020-01955-8\n10.3389/fimmu.2021.748447\n10.1111/aji.13372\n10.1186/s12958-018-0444-9\n10.1016/j.fertnstert.2007.03.074\n10.1016/j.rbmo.2022.07.008\n10.1093/humrep/deu292",
    "journal": "AJOG global reports",
    "keywords": [
        "Endometrial tuberculosis",
        "chronic endometritis",
        "microbial culture",
        "polymerase chain reaction"
    ],
    "methods": null,
    "publication_date": "2024-08-27",
    "pubmed_id": "39188580\n29477653\n31710184\n21749546\n30518389\n33323809\n15335255\n30843321\n28602480\n29477653\n28007229\n28090456\n35008911\n36511457\n25384850\n23087032\n26823918\n17244804\n16650954\n14766837\n20298269\n17141453\n33575432\n15472851\n22678036\n33641856\n36072071\n31074566\n28259137\n33025403\n34671363\n33155317\n30606202\n17531993\n36075850\n25385744\n36822566\n35621273\n27391900\n33308655\n31056314",
    "results": "In our study, PCR identified 318 cases (63.6%) positive for at least one of the 11 investigated pathogens, while culture-based methods detected 115 cases (23%). Predominant pathogens detected by PCR included ",
    "title": "Revolutionizing chronic endometritis diagnosis: real-time polymerase chain reaction unveils microbial pathogens in Indian women with abnormal bleeding and reproductive challenges.",
    "xml": "<Element 'PubmedArticle' at 0x7885029037e0>"
}