{
    "abstract": "Human monocyte-derived dendritic cells (moDC) are commonly used as a research tool to investigate interactions between antigen-presenting cells and T cells. Generation of these cells involves the isolation of CD14 positive\u00a0monocytes from peripheral blood and their in vitro differentiation into immature moDC by the cytokines GM-CSF and IL-4. Their functional characteristics can then be manipulated by maturing these cells with a cocktail of agents, which can be tailored to induce either immune activating or tolerogenic properties. Here, we describe a protocol for the generation of moDC with stable tolerogenic function, referred to as tolerogenic dendritic cells. These cells have been developed as an immunotherapeutic tool for the treatment of autoimmune disease but have also proven useful to dissect mechanisms of T cell tolerance induction in vitro.",
    "authors": [
        {
            "affiliation": "Translational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK. catharien.hilkens@newcastle.ac.uk.",
            "firstname": "Catharien M U",
            "initials": "CMU",
            "lastname": "Hilkens"
        },
        {
            "affiliation": "Translational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK.",
            "firstname": "Julie",
            "initials": "J",
            "lastname": "Diboll"
        },
        {
            "affiliation": "Translational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK.",
            "firstname": "Fiona",
            "initials": "F",
            "lastname": "Cooke"
        },
        {
            "affiliation": "Translational & Clinical Research Institute, Newcastle University, Newcastle-upon-Tyne, UK.",
            "firstname": "Amy E",
            "initials": "AE",
            "lastname": "Anderson"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
    "doi": "10.1007/978-1-0716-3135-5_31\n10.1084/jem.176.6.1693\n10.1084/jem.179.4.1109\n10.1084/jem.180.1.83\n10.1038/42030\n10.1182/blood.V90.5.1920\n10.1002/jlb.66.2.201\n10.1155/2015/471719\n10.3109/08830180903281193\n10.3389/fimmu.2018.02062\n10.1189/jlb.0608374\n10.1189/jlb.1107744\n10.1111/cei.12870\n10.1186/s12967-019-2128-4\n10.1136/ard.2009.126383\n10.1136/annrheumdis-2015-208456",
    "journal": "Methods in molecular biology (Clifton, N.J.)",
    "keywords": [
        "1,25-dihydroxy-vitamin D3",
        "Dendritic cells",
        "Dexamethasone",
        "Granulocyte-macrophage colony stimulating factor",
        "Human",
        "Interleukin-4",
        "Monocytes",
        "Peripheral blood mononuclear cells",
        "Tolerogenic\u00a0dendritic cells",
        "Toll-like receptor 4 ligand"
    ],
    "methods": null,
    "publication_date": "2023-04-28",
    "pubmed_id": "37106202\n1460426\n8145033\n8006603\n9285591\n9292525\n10449154\n20199240\n30298066\n18971286\n18430785\n27667787\n31727095\n20551157\n27117700",
    "results": null,
    "title": "In Vitro Generation of Human Tolerogenic Monocyte-Derived Dendritic Cells.",
    "xml": "<Element 'PubmedArticle' at 0x788505936fc0>"
}