{
    "abstract": "Bone marrow (BM) suppression is the most prevalent dose-limiting side effect of chemotherapy and radiotherapy. Exposure to ionizing radiation (IR) results in acute BM suppression and long-term BM injury. Gasdermin E (GSDME) is crucial for mediating apoptosis and pyroptosis during chemotherapy. However, its role in radiation-induced hematopoietic injury is not well established. Therefore, we aimed to investigate the role of GSDME on radiation-induced hematopoietic injury.\nWe established hematopoietic radiation injury models in C57BL/6 mice and Gsdme\nWe demonstrated that GSDME regulates the survival and differentiation of hematopoietic stem cells (HSCs). The knockout of GSDME reduced the number and proliferation of HSCs and shortened the survival time of mice post IR. Additionally, GSDME knockout protected LSK (Lin-Sca1+c-kit+) cells, long-term HSCs (LT-HSCs), granulocyte-monocyte progenitors (GMPs), and myeloid cells (M cells) from IR injuries during acute BM suppression. Furthermore, GSDME knockout protected LSK cells, LT-HSCs, GMPs and M cells, alleviated the proliferation inhibition of hematopoietic progenitor cells (HPCs) and exacerbated lymphocyte damage during long-term BM injury.\nGSDME is vital for the survival and differentiation of HSCs, and its absence promotes myeloid-biased differentiation postirradiation. These findings highlight the critical role of GSDME in radiation-induced hematopoietic injury, particularly in the myeloid differentiation of HSCs.",
    "authors": [
        {
            "affiliation": "Department of Oncology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, China.\nKey Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Engineering Research Center for Laborator Animal Models of Human Critical Diseases, National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.",
            "firstname": "Lulu",
            "initials": "L",
            "lastname": "Su"
        },
        {
            "affiliation": "Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Tianjin, China.",
            "firstname": "Yinping",
            "initials": "Y",
            "lastname": "Dong"
        },
        {
            "affiliation": "Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Engineering Research Center for Laborator Animal Models of Human Critical Diseases, National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.",
            "firstname": "Bowen",
            "initials": "B",
            "lastname": "Guan"
        },
        {
            "affiliation": "Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Engineering Research Center for Laborator Animal Models of Human Critical Diseases, National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.",
            "firstname": "Yuquan",
            "initials": "Y",
            "lastname": "Wang"
        },
        {
            "affiliation": "Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Engineering Research Center for Laborator Animal Models of Human Critical Diseases, National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.",
            "firstname": "Yanhua",
            "initials": "Y",
            "lastname": "Lu"
        },
        {
            "affiliation": "Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Tianjin, China.",
            "firstname": "Xinyue",
            "initials": "X",
            "lastname": "Wang"
        },
        {
            "affiliation": "Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Tianjin, China.",
            "firstname": "Wenxuan",
            "initials": "W",
            "lastname": "Li"
        },
        {
            "affiliation": "Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Tianjin, China.",
            "firstname": "Qidong",
            "initials": "Q",
            "lastname": "Huo"
        },
        {
            "affiliation": "Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Engineering Research Center for Laborator Animal Models of Human Critical Diseases, National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.",
            "firstname": "Aimin",
            "initials": "A",
            "lastname": "Meng"
        },
        {
            "affiliation": "Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Tianjin, China.",
            "firstname": "Deguan",
            "initials": "D",
            "lastname": "Li"
        }
    ],
    "conclusions": "GSDME is vital for the survival and differentiation of HSCs, and its absence promotes myeloid-biased differentiation postirradiation. These findings highlight the critical role of GSDME in radiation-induced hematopoietic injury, particularly in the myeloid differentiation of HSCs.",
    "copyrights": "Copyright \u00a9 2025 Su, Dong, Guan, Wang, Lu, Wang, Li, Huo, Meng and Li.",
    "doi": "10.3389/fcell.2025.1544320\n10.1667/rr13887.1\n10.1186/s13046-022-02533-9\n10.1016/s0301-472x(01)00685-3\n10.1038/ni.3204\n10.1038/s41420-020-00349-0\n10.1371/journal.pone.0062921\n10.1158/1078-0432.ccr-18-1478\n10.1016/0360-3016(94)00430-s\n10.1016/j.stem.2010.06.014\n10.1038/s41580-019-0103-9\n10.1038/s41467-019-09397-2\n10.1038/ncomms14128\n10.1182/blood-2013-07-515619\n10.1016/j.canlet.2021.12.034\n10.1182/blood.v92.6.1950\n10.1177/00220345211073072\n10.1038/nature22393\n10.1182/blood-2005-04-1418\n10.1016/j.bbrc.2017.11.156\n10.1038/s41419-019-1441-4\n10.1007/s10495-019-01515-1",
    "journal": "Frontiers in cell and developmental biology",
    "keywords": [
        "GSDME",
        "acute bone marrow suppression",
        "ionizing radiation",
        "long-term bone marrow injury",
        "myeloid-biased differentiation"
    ],
    "methods": null,
    "publication_date": "2025-04-21",
    "pubmed_id": "40256765\n25635345\n36411454\n11532345\n26147687\n33133646\n23646161\n30061362\n7713791\n20619762\n30745579\n30976076\n28045099\n24622326\n34979164\n3888044\n9731052\n35148659\n28459430\n16150936\n29183726\n30804337\n30710195",
    "results": "We demonstrated that GSDME regulates the survival and differentiation of hematopoietic stem cells (HSCs). The knockout of GSDME reduced the number and proliferation of HSCs and shortened the survival time of mice post IR. Additionally, GSDME knockout protected LSK (Lin-Sca1+c-kit+) cells, long-term HSCs (LT-HSCs), granulocyte-monocyte progenitors (GMPs), and myeloid cells (M cells) from IR injuries during acute BM suppression. Furthermore, GSDME knockout protected LSK cells, LT-HSCs, GMPs and M cells, alleviated the proliferation inhibition of hematopoietic progenitor cells (HPCs) and exacerbated lymphocyte damage during long-term BM injury.",
    "title": "GSDME knockout alleviates hematopoietic stem cell irradiation injury and aggravates myeloid-biased differentiation.",
    "xml": "<Element 'PubmedArticle' at 0x788503953bf0>"
}