{
    "abstract": "Caprolactamase is the first enzyme in the caprolactam degradation pathway of Pseudomonas jessenii. It is composed of two subunits (CapA and CapB) and sequence-related to other ATP-dependent enzymes involved in lactam hydrolysis, like 5-oxoprolinases and hydantoinases. Low sequence similarity also exists with ATP-dependent acetone- and acetophenone carboxylases. The caprolactamase was produced in Escherichia coli, isolated by His-tag affinity chromatography, and subjected to functional and structural studies. Activity toward caprolactam required ATP and was dependent on the presence of bicarbonate in the assay buffer. The hydrolysis product was identified as 6-aminocaproic acid. Quantum mechanical modeling indicated that the hydrolysis of caprolactam was highly disfavored (\u0394G",
    "authors": [
        {
            "affiliation": "Biotransformation and Biocatalysis, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.",
            "firstname": "Antonija",
            "initials": "A",
            "lastname": "Marjanovic"
        },
        {
            "affiliation": "Biotransformation and Biocatalysis, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.",
            "firstname": "Henri\u00ebtte J",
            "initials": "HJ",
            "lastname": "Rozeboom"
        },
        {
            "affiliation": "Biotransformation and Biocatalysis, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.",
            "firstname": "Meintje S",
            "initials": "MS",
            "lastname": "de Vries"
        },
        {
            "affiliation": "Biomolecular Chemistry and Catalysis, Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands.",
            "firstname": "Clemens",
            "initials": "C",
            "lastname": "Mayer"
        },
        {
            "affiliation": "Biotransformation and Biocatalysis, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.",
            "firstname": "Marleen",
            "initials": "M",
            "lastname": "Otzen"
        },
        {
            "affiliation": null,
            "firstname": "Hein J",
            "initials": "HJ",
            "lastname": "Wijma"
        },
        {
            "affiliation": "Biotransformation and Biocatalysis, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, The Netherlands.",
            "firstname": "Dick B",
            "initials": "DB",
            "lastname": "Janssen"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2021 The Authors. Proteins: Structure, Function, and Bioinformatics published by Wiley Periodicals LLC.",
    "doi": "10.1002/prot.26082",
    "journal": "Proteins",
    "keywords": [
        "5-oxoproline",
        "6-aminocaproic acid",
        "carboxylase",
        "hydrolase",
        "lactamase",
        "nylon 6",
        "phosphorylation"
    ],
    "methods": null,
    "publication_date": "2021-04-08",
    "pubmed_id": "33826169\n6386341\n23974491\n9688819\n29850960\n5289242\n3827889\n28775283\n28054554\n31162815\n22020645\n12003937\n3911006\n26602\n9925605\n1480111\n1730246\n3611103\n27477828\n14993790\n8943290\n20413906\n20402795\n22447903\n1732229\n31841635\n7744042\n28830929\n31520514\n3911007\n2563377\n239948\n20047908\n24753421\n1375309\n19366259\n32857966\n32497192\n27295634\n29860391\n1323828\n28722183\n15337755\n19890726\n12135362\n28336403\n1445229\n14454713\n9818189\n19213731\n26020841\n25372694\n7781919\n19363495\n20124692\n5700707\n19461840\n24130308\n24076763\n20383002\n24269527\n22477769\n20057044\n19768677\n23093919\n21994221",
    "results": null,
    "title": "Catalytic and structural properties of ATP-dependent caprolactamase from Pseudomonas jessenii.",
    "xml": "<Element 'PubmedArticle' at 0x78850577efc0>"
}