{
    "abstract": "Acute Kidney Injury (AKI), a prevalent complication of Liver Transplantation (LT) that occurs during the perioperative period has been established to profoundly impact the prognosis of transplant recipients. This study aimed to investigate the mechanism of the hepatic IRI-induced AKI and to identify potential therapeutic targets for treating this condition and improving the prognosis of LT patients.\nAn integrated transcriptomics and proteomics approach was employed to investigate transcriptional and proteomic alterations in hepatic IRI-induced AKI and the hypoxia-reoxygenation (H/R) model using TCMK-1 cells and the hepatic IRI-induced AKI mouse model using male C57BL/6\u00a0J mice were employed to elucidate the underlying mechanisms. Hematoxylin-eosin staining, reverse transcription quantitative polymerase chain reaction, enzyme-linked immunosorbent assay and Western blot were used to assess the effect of Rosiglitazone\u00a0(RGZ) on hepatic IRI-induced AKI in vitro and in vivo.\nAccording to the results, 322 genes and 128 proteins were differentially expressed between the sham and AKI groups. Furthermore, Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway analyses revealed significant enrichment in pathways related to amino acid and lipid metabolism. Additionally, the Protein-Protein Interaction (PPI) network analysis of the kidney tissues obtained from a hepatic IRI-induced AKI mouse model highlighted arachidonic acid metabolism as the most prominent pathway. Animal and cellular analyses further revealed that RGZ, a PPAR-\u03b3 agonist, could inhibit the expression of the PPAR-\u03b3/NF-\u03baB signaling pathway-associated proteins in in vitro and in vivo.\nThese findings collectively suggest that RGZ ameliorates hepatic IRI-induced AKI via PPAR-\u03b3/NF-\u03baB signaling pathway modulation, highlighting PPAR-\u03b3 as a crucial therapeutic target for AKI prevention post-LT.",
    "authors": [
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.\nDepartment of General Surgery and Trauma Surgery, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, Chongqing, 400014, China.",
            "firstname": "Xiaoyan",
            "initials": "X",
            "lastname": "Qin"
        },
        {
            "affiliation": "The First Clinical College of Chongqing Medical University, Chongqing, 400046, China.",
            "firstname": "Zhengli",
            "initials": "Z",
            "lastname": "Tan"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Qi",
            "initials": "Q",
            "lastname": "Li"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Shiyi",
            "initials": "S",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Dingheng",
            "initials": "D",
            "lastname": "Hu"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Denghui",
            "initials": "D",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Liangxu",
            "initials": "L",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Baoyong",
            "initials": "B",
            "lastname": "Zhou"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Rui",
            "initials": "R",
            "lastname": "Liao"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.",
            "firstname": "Zhongjun",
            "initials": "Z",
            "lastname": "Wu"
        },
        {
            "affiliation": "Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China. liuyanyao@hospital.cqmu.edu.cn.",
            "firstname": "Yanyao",
            "initials": "Y",
            "lastname": "Liu"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.",
    "doi": "10.1007/s00011-024-01929-x\n10.1016/j.jhep.2023.03.007\n10.1093/bjs/znad243\n10.1016/S2468-1253(23)00363-1\n10.1016/j.cgh.2023.06.010\n10.1186/s12882-021-02360-8\n10.1002/lt.26417\n10.1080/0886022X.2021.1945462\n10.12659/AOT.937535\n10.1097/TP.0000000000002584\n10.3389/ti.2023.10887\n10.1097/TP.0000000000002085\n10.1111/aas.13556\n10.1056/NEJMp1309610\n10.1016/j.toxlet.2016.11.012\n10.1016/j.jcmgh.2021.01.003\n10.1161/STROKEAHA.115.010091\n10.1139/cjpp-2015-0230\n10.1016/j.intimp.2021.107809\n10.1016/j.intimp.2021.107604\n10.1038/s41419-020-2648-0\n10.1038/ncomms8443\n10.4254/wjh.v14.i7.1365\n10.1093/ndt/gfx299\n10.1186/s13059-014-0550-8\n10.1002/imt2.12\n10.12659/MSM.923864\n10.1038/s41598-023-31757-8\n10.1097/TP.0000000000003331\n10.1002/lt.24488\n10.1371/journal.pone.0237503\n10.1038/s41598-023-34372-9\n10.1002/lt.24728\n10.1002/mco2.363\n10.3390/ijms241814209\n10.1681/ASN.2017030252\n10.3390/ijms20153683\n10.1155/2022/6852661\n10.3390/ijms241411503\n10.1016/j.phymed.2021.153818\n10.1161/STROKEAHA.113.002470",
    "journal": "Inflammation research : official journal of the European Histamine Research Society ... [et al.]",
    "keywords": [
        "Acute Kidney Injury",
        "PPAR-\u03b3",
        "Proteomics",
        "Rosiglitazone",
        "Transcriptomics"
    ],
    "methods": null,
    "publication_date": "2024-08-08",
    "pubmed_id": "39112648\n37208100\n37548041\n37625863\n33888081\n35100664\n34261422\n36164260\n30720690\n36744052\n29329188\n32020588\n23992603\n27866976\n33444819\n26243225\n26394869\n34182323\n33839577\n32532961\n26068133\n36158922\n29126308\n25516281\n38868573\n32681793\n32467477\n27302834\n32810154\n37244919\n28124458\n37746665\n37762512\n28701518\n31357612\n30560267\n34040498\n35646124\n37511261\n34798521\n24135932",
    "results": "According to the results, 322 genes and 128 proteins were differentially expressed between the sham and AKI groups. Furthermore, Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway analyses revealed significant enrichment in pathways related to amino acid and lipid metabolism. Additionally, the Protein-Protein Interaction (PPI) network analysis of the kidney tissues obtained from a hepatic IRI-induced AKI mouse model highlighted arachidonic acid metabolism as the most prominent pathway. Animal and cellular analyses further revealed that RGZ, a PPAR-\u03b3 agonist, could inhibit the expression of the PPAR-\u03b3/NF-\u03baB signaling pathway-associated proteins in in vitro and in vivo.",
    "title": "Rosiglitazone attenuates Acute Kidney Injury from hepatic ischemia-reperfusion in mice by inhibiting arachidonic acid metabolism through the PPAR-\u03b3/NF-\u03baB pathway.",
    "xml": "<Element 'PubmedArticle' at 0x788502ae0f40>"
}