{
    "abstract": "Mycobacterium tuberculosis (Mtb) infection remains a major public health concern. The STING (stimulator of interferon genes) pathway contributes to the cytosolic surveillance of host cells. Most studies on the role of STING activation in Mtb infection have focused on macrophages. Moreover, a detailed investigation of the role of STING during Mtb infection in vivo is required. Here, we deciphered the involvement of STING in the activation of dendritic cells (DCs) and the host response to Mtb infection in vivo. In DCs, this adaptor molecule was important for Ifn-\u03b2 expression and IL-12 production as well as for the surface expression of the activation markers CD40 and CD86. We also documented that Mtb DNA induces STING activation in murine fibroblasts. In vivo Mtb aerogenic infection induced the upregulation of the STING and cGAS (cyclic GMP-AMP synthase) genes, and Ifn-\u03b2 pulmonary expression was dependent on both sensors. However, mice deficient for STING or cGAS presented a similar outcome to wild-type controls, with no major alterations in body weight gain, bacterial burden, or survival. Lung inflammation, proinflammatory cytokine production, and inflammatory cell recruitment were similar in STING- and cGAS-deficient mice compared to wild-type controls. In summary, although the STING pathway seems to be crucial for DC activation during Mtb infection, it is dispensable for host protection in vivo.",
    "authors": [
        {
            "affiliation": "Centre National de la Recherche Scientifique (CNRS), UMR7355, Orleans, France.\nExperimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France.\nDepartamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.\nInstituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT), Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Minist\u00e9rio de Ci\u00eancia Tecnologia e Inova\u00e7\u00e3o Salvador, Bahia, Brazil.",
            "firstname": "Fabio V",
            "initials": "FV",
            "lastname": "Marinho"
        },
        {
            "affiliation": "Centre National de la Recherche Scientifique (CNRS), UMR7355, Orleans, France.\nExperimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France.",
            "firstname": "Sulayman",
            "initials": "S",
            "lastname": "Benmerzoug"
        },
        {
            "affiliation": "Centre National de la Recherche Scientifique (CNRS), UMR7355, Orleans, France.\nExperimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France.",
            "firstname": "Stephanie",
            "initials": "S",
            "lastname": "Rose"
        },
        {
            "affiliation": "Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.",
            "firstname": "Priscila C",
            "initials": "PC",
            "lastname": "Campos"
        },
        {
            "affiliation": "Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.",
            "firstname": "Jo\u00e3o T",
            "initials": "JT",
            "lastname": "Marques"
        },
        {
            "affiliation": "Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florian\u00f3polis, Brazil.",
            "firstname": "Andr\u00e9",
            "initials": "A",
            "lastname": "B\u00e1fica"
        },
        {
            "affiliation": "Department of Cell Biology, University of Miami, Miami, Florida, USA.",
            "firstname": "Glen",
            "initials": "G",
            "lastname": "Barber"
        },
        {
            "affiliation": "Centre National de la Recherche Scientifique (CNRS), UMR7355, Orleans, France.\nExperimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France.",
            "firstname": "Bernhard",
            "initials": "B",
            "lastname": "Ryffel"
        },
        {
            "affiliation": "Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.\nInstituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT), Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Minist\u00e9rio de Ci\u00eancia Tecnologia e Inova\u00e7\u00e3o Salvador, Bahia, Brazil.",
            "firstname": "Sergio C",
            "initials": "SC",
            "lastname": "Oliveira"
        },
        {
            "affiliation": "Centre National de la Recherche Scientifique (CNRS), UMR7355, Orleans, France.\nExperimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France.",
            "firstname": "Valerie F J",
            "initials": "VFJ",
            "lastname": "Quesniaux"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2018 S. Karger AG, Basel.",
    "doi": "10.1159/000488952",
    "journal": "Journal of innate immunity",
    "keywords": [
        "Dendritic cells",
        "Mycobacterium tuberculosis",
        "STING",
        "cGAS"
    ],
    "methods": null,
    "publication_date": "2018-05-24",
    "pubmed_id": "29791904\n19776740\n27672085\n23258413\n20890285\n23142775\n22000020\n24600004\n22901810\n27334590\n27792766\n27566796\n17480182\n28625530\n25539957\n22607800\n25730264\n26048137\n26048138\n26048136\n23132945\n23989956\n8009221\n8456301\n9223320\n15599404\n18724357\n16912041\n25320273\n25517616\n10092807\n25400917\n27763255\n18762264\n16365150\n28424703\n27500737\n15618214\n28801234\n24913717\n27716849\n7642273",
    "results": null,
    "title": "The cGAS/STING Pathway Is Important for Dendritic Cell Activation but Is Not Essential to Induce Protective Immunity against Mycobacterium tuberculosis Infection.",
    "xml": "<Element 'PubmedArticle' at 0x78850596ca90>"
}