{
    "abstract": "Aurora B, together with IN-box, the C-terminal part of INCENP, forms an enzymatic complex that ensures faithful cell division. The [Aurora B/IN-box] complex is activated by autophosphorylation in the Aurora B activation loop and in IN-box, but it is not clear how these phosphorylations activate the enzyme. We used a combination of experimental and computational studies to investigate the effects of phosphorylation on the molecular dynamics and structure of [Aurora B/IN-box]. In addition, we generated partially phosphorylated intermediates to analyze the contribution of each phosphorylation independently. We found that the dynamics of Aurora and IN-box are interconnected, and IN-box plays both positive and negative regulatory roles depending on the phosphorylation status of the enzyme complex. Phosphorylation in the activation loop of Aurora B occurs intramolecularly and prepares the enzyme complex for activation, but two phosphorylated sites are synergistically responsible for full enzyme activity.",
    "authors": [
        {
            "affiliation": "Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Faculty of Medicine, Oslo, Norway.",
            "firstname": "Dario",
            "initials": "D",
            "lastname": "Segura-Pe\u00f1a"
        },
        {
            "affiliation": "Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Faculty of Medicine, Oslo, Norway.\nDepartment of Biosciences, University of Oslo, Oslo, Norway.\nHylleraas Centre for Quantum Molecular Sciences, University of Oslo, Oslo, Norway.",
            "firstname": "Oda",
            "initials": "O",
            "lastname": "Hovet"
        },
        {
            "affiliation": "Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Faculty of Medicine, Oslo, Norway.",
            "firstname": "Hemanga",
            "initials": "H",
            "lastname": "Gogoi"
        },
        {
            "affiliation": "Department of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.",
            "firstname": "Jennine",
            "initials": "J",
            "lastname": "Dawicki-McKenna"
        },
        {
            "affiliation": "Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Faculty of Medicine, Oslo, Norway.",
            "firstname": "Stine Malene",
            "initials": "SM",
            "lastname": "Hansen W\u00f8ien"
        },
        {
            "affiliation": "Hylleraas Centre for Quantum Molecular Sciences, University of Oslo, Oslo, Norway.\nDepartment of Chemistry, University of Oslo, Oslo, Norway.",
            "firstname": "Manuel",
            "initials": "M",
            "lastname": "Carrer"
        },
        {
            "affiliation": "Department of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.",
            "firstname": "Ben E",
            "initials": "BE",
            "lastname": "Black"
        },
        {
            "affiliation": "Hylleraas Centre for Quantum Molecular Sciences, University of Oslo, Oslo, Norway.\nDepartment of Chemistry, University of Oslo, Oslo, Norway.",
            "firstname": "Michele",
            "initials": "M",
            "lastname": "Cascella"
        },
        {
            "affiliation": "Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Faculty of Medicine, Oslo, Norway.",
            "firstname": "Nikolina",
            "initials": "N",
            "lastname": "Sekulic"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2023, Segura-Pe\u00f1a et al.",
    "doi": "10.7554/eLife.85328\n10.1038/s41467-019-11085-0\n10.1126/science.1251121\n10.1002/iub.2057\n10.1006/jmbi.2000.3859\n10.1016/s1097-2765(03)00392-7\n10.1074/jbc.C200307200\n10.1126/science.1130258\n10.1038/nrm3474\n10.1016/j.jasms.2009.05.017\n10.1038/nchembio.2296\n10.1016/j.molcel.2015.10.013\n10.1074/jbc.M111.312090\n10.1126/scisignal.2003910\n10.1021/jm3008954\n10.1063/1.470117\n10.1021/bi035943y\n10.1083/jcb.201301131\n10.1038/ncomms6939\n10.1038/nature06923\n10.1007/978-1-61737-967-3\n10.1002/anie.201704654\n10.1021/jacs.9b06020\n10.1002/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H\n10.1103/physreve.62.8438\n10.1201/9780367806934\n10.1091/mbc.e02-11-0769\n10.1002/jps.22432\n10.1074/jbc.M805581200\n10.1038/nmeth.4067\n10.1016/0263-7855(96)00018-5\n10.1038/srep28528\n10.1080/13543776.2021.1890027\n10.1063/1.445869\n10.1073/pnas.1014664107\n10.1016/j.ceb.2009.01.004\n10.1073/pnas.0807988105\n10.1016/j.tibs.2015.09.002\n10.3389/fonc.2015.00225\n10.1016/j.ymeth.2004.03.005\n10.1083/jcb.200204155\n10.1073/pnas.1721441115\n10.7554/eLife.22175\n10.1093/bioinformatics/btaa677\n10.1016/j.tibs.2019.09.007\n10.1042/BCJ20170771\n10.1126/science.1167000\n10.1042/BST20130250\n10.1063/1.463940\n10.1038/s41592-019-0459-y\n10.1038/nchembio.452\n10.1073/pnas.1102701108\n10.1016/bs.mie.2015.06.035\n10.1016/j.gde.2003.11.006\n10.1080/00268978400101201\n10.1063/1.328693\n10.1016/j.sbi.2021.07.006\n10.7554/eLife.36656\n10.2174/092986711794480203\n10.1093/bioinformatics/btt055\n10.1016/0076-6879(83)99034-1\n10.7554/eLife.32766\n10.1016/j.sbi.2016.01.005\n10.1016/j.molcel.2005.03.031\n10.1107/S2053230X14002118\n10.1002/pro.2081\n10.1007/978-1-60761-795-2\n10.1016/j.ijms.2012.10.013\n10.1074/jbc.M109.061820\n10.1016/j.tibs.2010.09.006\n10.1128/jb.179.20.6378-6382.1997\n10.1021/jasms.1c00271\n10.1038/nature11271\n10.1083/jcb.201302123\n10.1083/jcb.200604032\n10.1002/jcc.20291\n10.1073/pnas.1319482110\n10.1083/jcb.201103044\n10.1074/jbc.M110.156505\n10.1038/s41467-019-08655-7\n10.1021/acs.chemrev.5b00562\n10.1074/jbc.M308196200\n10.1073/pnas.1318899111\n10.1021/acs.accounts.5b00001\n10.1126/science.abc2754\n10.7554/eLife.10644\n10.1073/pnas.1917219117",
    "journal": "eLife",
    "keywords": [
        "Aurora B",
        "E. coli",
        "allostery",
        "autoactivation",
        "biochemistry",
        "cell division",
        "chemical biology",
        "conformational dynamics",
        "molecular biophysics",
        "protein kinase",
        "structural biology",
        "xenopus"
    ],
    "methods": null,
    "publication_date": "2023-05-25",
    "pubmed_id": "37227118\n31320618\n24925910\n31063633\n10864511\n14580337\n12048181\n16973882\n23175282\n19631556\n28166210\n26626480\n22094468\n23821772\n22920039\n14730962\n23712260\n25600932\n18463638\n21125382\n28700101\n31449409\n11138145\n12925766\n21491437\n18984590\n27819658\n8744570\n27339427\n33573401\n21097701\n19185479\n18787129\n26481499\n26528436\n15283915\n12177045\n29666261\n28445123\n32722756\n31690482\n29946042\n19150808\n24450642\n31249422\n20890288\n21471451\n26791986\n15108802\n34425481\n29901437\n21143115\n23407358\n6316096\n29465396\n26820051\n15866179\n24598913\n22544544\n20811987\n23439742\n19965870\n20971646\n9335286\n35099954\n22801505\n23960144\n16769825\n16211538\n24191053\n21844210\n21098037\n30778078\n26807783\n12907671\n24550275\n25803188\n33004676\n26765564\n32371482",
    "results": null,
    "title": "The structural basis of the multi-step allosteric activation of Aurora B kinase.",
    "xml": "<Element 'PubmedArticle' at 0x788505cbacf0>"
}