{
    "abstract": "COVID-19 is an ongoing global pandemic. There is an urgent need for identification and understanding of clinical and laboratory parameters related to progression towards a severe and fatal form of this illness, often preceded by a so-called cytokine-storm syndrome (CSS). Therefore, we explored the hemocytometric characteristics of COVID-19 patients in relation to the deteriorating clinical condition CSS, using the Sysmex XN-10 hematology analyzer.\nFrom March 1st till May 16th, 2020, all patients admitted to our hospital with respiratory complaints and suspected for COVID-19 were included (n=1,140 of whom n=533 COVID-19 positive). The hemocytometric parameters of immunocompetent cells in peripheral blood (neutrophils [NE], lymphocytes [LY] and monocytes [MO]) obtained upon admission to the emergency department (ED) of COVID-19 positive patients were compared with those of the COVID-19 negative ones. Moreover, patients with CSS (n=169) were compared with COVID-19 positive patients without CSS, as well as with COVID-19 negative ones.\nIn addition to a significant reduction in leukocytes, thrombocytes and absolute neutrophils, it appeared that\u00a0lymphocytes-forward scatter (LY-FSC), and reactive lymphocytes (RE-LYMPHO)/leukocytes were higher in COVID-19-positive than negative patients. At the moment\u00a0of presentation, COVID-19 positive patients with\u00a0CSS had\u00a0different neutrophils-side fluorescence (NE-SFL), neutrophils-forward scatter (NE-FSC), LY-FSC, RE-LYMPHO/lymphocytes, antibody-synthesizing (AS)-LYMPHOs, high fluorescence lymphocytes (HFLC), MO-SSC, MO-SFL, and Reactive (RE)-MONOs. Finally, absolute eosinophils, basophils, lymphocytes, monocytes and MO-FSC were lower in patients with CSS.\nHemocytometric parameters indicative of changes in immunocompetent peripheral blood cells and measured at admission to the ED were associated with COVID-19 with and without CSS.",
    "authors": [
        {
            "affiliation": "Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Remy J H",
            "initials": "RJH",
            "lastname": "Martens"
        },
        {
            "affiliation": "Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Arjan J",
            "initials": "AJ",
            "lastname": "van Adrichem"
        },
        {
            "affiliation": "Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Nadine J A",
            "initials": "NJA",
            "lastname": "Mattheij"
        },
        {
            "affiliation": "Department of Internal Medicine, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Calvin G",
            "initials": "CG",
            "lastname": "Brouwer"
        },
        {
            "affiliation": "Department of Internal Medicine, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Daan J L",
            "initials": "DJL",
            "lastname": "van Twist"
        },
        {
            "affiliation": "Department of Pulmonology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Jasper J C R",
            "initials": "JJCR",
            "lastname": "Broerse"
        },
        {
            "affiliation": "Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "C\u00e9sar",
            "initials": "C",
            "lastname": "Magro-Checa"
        },
        {
            "affiliation": "Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Christel M P",
            "initials": "CMP",
            "lastname": "van Dongen"
        },
        {
            "affiliation": "Department of Pulmonology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "R\u00e9my L M",
            "initials": "RLM",
            "lastname": "Mostard"
        },
        {
            "affiliation": "Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.\nDepartment of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.",
            "firstname": "Sofia",
            "initials": "S",
            "lastname": "Ramiro"
        },
        {
            "affiliation": "Department of Rheumatology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.\nDepartment of Clinical Immunology and Rheumatology, Amsterdam University Medical Center, Amsterdam, The Netherlands.",
            "firstname": "Robert B M",
            "initials": "RBM",
            "lastname": "Landew\u00e9"
        },
        {
            "affiliation": "Department of Clinical Chemistry and Hematology, Zuyderland Medical Center, Heerlen & Sittard, The Netherlands.",
            "firstname": "Math P G",
            "initials": "MPG",
            "lastname": "Leers"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2020 Remy J. H. Martens et al., published by De Gruyter, Berlin/Boston.",
    "doi": "10.1515/cclm-2020-1529",
    "journal": "Clinical chemistry and laboratory medicine",
    "keywords": [
        "COVID-19",
        "SARS-CoV-2",
        "cell population data",
        "cytokine storm syndrome",
        "hemocytometry"
    ],
    "methods": null,
    "publication_date": "2021-02-09",
    "pubmed_id": "33554540",
    "results": "In addition to a significant reduction in leukocytes, thrombocytes and absolute neutrophils, it appeared that\u00a0lymphocytes-forward scatter (LY-FSC), and reactive lymphocytes (RE-LYMPHO)/leukocytes were higher in COVID-19-positive than negative patients. At the moment\u00a0of presentation, COVID-19 positive patients with\u00a0CSS had\u00a0different neutrophils-side fluorescence (NE-SFL), neutrophils-forward scatter (NE-FSC), LY-FSC, RE-LYMPHO/lymphocytes, antibody-synthesizing (AS)-LYMPHOs, high fluorescence lymphocytes (HFLC), MO-SSC, MO-SFL, and Reactive (RE)-MONOs. Finally, absolute eosinophils, basophils, lymphocytes, monocytes and MO-FSC were lower in patients with CSS.",
    "title": "Hemocytometric characteristics of COVID-19 patients with and without cytokine storm syndrome on the sysmex XN-10 hematology analyzer.",
    "xml": "<Element 'PubmedArticle' at 0x788505b09990>"
}