{
    "abstract": "Microinjection of capacitated sperm into the perivitelline space of oocyte was offered to one couple with persistent infertility of mixed origin. The husband's semen was subnormal, whereas his wife had definitive tubal occlusion and polycystic ovaries. Four previous in vitro fertilization (IVF) attempts were performed but no fertilization was obtained. After superovulation, 13 oocytes were collected. Ten were submitted to microinjection and two were damaged during the procedure. One of the remaining eight had two pronuclei 18 hr after microinjection and progressed to a four-cell embryo after 48 hr. After reimplantation, a normal pregnancy was initiated, caryotype (46XX) was checked at 17 weeks. A normal and healthy girl has been delivered at term.",
    "authors": [
        {
            "affiliation": "D\u00e9partement de F\u00e9condation in vitro, CMCO BP120, Schiltigheim, France.",
            "firstname": "C",
            "initials": "C",
            "lastname": "Wittemer"
        },
        {
            "affiliation": null,
            "firstname": "L",
            "initials": "L",
            "lastname": "Moreau"
        },
        {
            "affiliation": null,
            "firstname": "P",
            "initials": "P",
            "lastname": "Dellenbach"
        },
        {
            "affiliation": null,
            "firstname": "P",
            "initials": "P",
            "lastname": "Gerlinger"
        },
        {
            "affiliation": null,
            "firstname": "M A",
            "initials": "MA",
            "lastname": "Le Meur"
        },
        {
            "affiliation": null,
            "firstname": "P",
            "initials": "P",
            "lastname": "Chambon"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.1007/BF01130809",
    "journal": "Journal of in vitro fertilization and embryo transfer : IVF",
    "keywords": [],
    "methods": null,
    "publication_date": "1991-08-01",
    "pubmed_id": "1753169\n2226918\n2910709\n2404802\n2901626\n4809692\n3131387\n6149412\n821667\n3385161\n2745674\n6145902\n4916973\n2870385",
    "results": null,
    "title": "A case of human pregnancy after microinjection of capacitated sperm into the perivitelline space.",
    "xml": "<Element 'PubmedArticle' at 0x788505c227f0>"
}