{
    "abstract": "To identify the dynamics of neutrophil autoimmunity, here we focus on anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis and perform single-cell transcriptome and surface proteome analyses on peripheral white blood cells from patients with new-onset microscopic polyangiitis (MPA). Compared with controls, two neutrophil populations, immature neutrophils and neutrophils with type II interferon signature genes (Neu_T2ISG), are increased in patients with MPA. Trajectory and cell-cell interaction analyses identify Neu_T2ISG as a subset that differentiates from mature neutrophils upon stimulation with IFN-\u03b3 and TNF, which synergize to induce myeloperoxidase and Fc\u03b3 receptors expression on the neutrophil cell surface and promote ANCA-induced neutrophil extracellular trap formation. Case-by-case analysis indicates that patients with a high proportion of the Neu_T2ISG subset are associated with persistent vasculitis symptoms. A larger cohort analysis shows that serum IFN-\u03b3 levels at disease onset correlate with susceptibility to disease relapse. Our findings thus identify neutrophil diversity at the single cell level and implicate a biomarker for predicting relapse in small vessel vasculitis.",
    "authors": [
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. nishide@imed3.med.osaka-u.ac.jp.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan. nishide@imed3.med.osaka-u.ac.jp.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. nishide@imed3.med.osaka-u.ac.jp.",
            "firstname": "Masayuki",
            "initials": "M",
            "lastname": "Nishide"
        },
        {
            "affiliation": "Department of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nJoint Research Chair of Innovative Drug Discovery in Immunology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nResearch Division, Chugai Pharmaceutical Co. Ltd, Yokohama, Kanagawa, Japan.",
            "firstname": "Kei",
            "initials": "K",
            "lastname": "Nishimura"
        },
        {
            "affiliation": "Department of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nJoint Research Chair of Innovative Drug Discovery in Immunology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nResearch Division, Chugai Pharmaceutical Co. Ltd, Yokohama, Kanagawa, Japan.",
            "firstname": "Hiroaki",
            "initials": "H",
            "lastname": "Matsushita"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Shoji",
            "initials": "S",
            "lastname": "Kawada"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Hiroshi",
            "initials": "H",
            "lastname": "Shimagami"
        },
        {
            "affiliation": "Department of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nJoint Research Chair of Innovative Drug Discovery in Immunology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nResearch Division, Chugai Pharmaceutical Co. Ltd, Yokohama, Kanagawa, Japan.",
            "firstname": "Shoichi",
            "initials": "S",
            "lastname": "Metsugi"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Yasuhiro",
            "initials": "Y",
            "lastname": "Kato"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Takahiro",
            "initials": "T",
            "lastname": "Kawasaki"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Kohei",
            "initials": "K",
            "lastname": "Tsujimoto"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Statistical Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nLaboratory for Systems Genetics, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, Japan.",
            "firstname": "Ryuya",
            "initials": "R",
            "lastname": "Edahiro"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Statistical Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Yuya",
            "initials": "Y",
            "lastname": "Shirai"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Eri",
            "initials": "E",
            "lastname": "Itotagawa"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Maiko",
            "initials": "M",
            "lastname": "Naito"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Statistical Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Yuji",
            "initials": "Y",
            "lastname": "Yamamoto"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Kazuki",
            "initials": "K",
            "lastname": "Matsukawa"
        },
        {
            "affiliation": "Joint Research Chair of Innovative Drug Discovery in Immunology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nResearch Division, Chugai Pharmaceutical Co. Ltd, Yokohama, Kanagawa, Japan.",
            "firstname": "Ryusuke",
            "initials": "R",
            "lastname": "Omiya"
        },
        {
            "affiliation": "Department of Statistical Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nLaboratory for Systems Genetics, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, Japan.\nIntegrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), Osaka University, Suita, Osaka, Japan.\nCenter for Infectious Diseases for Education and Research (CiDER), Osaka University, Suita, Osaka, Japan.\nStatistical Immunology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Genome Informatics, Graduate School of Medicine, the University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.",
            "firstname": "Yukinori",
            "initials": "Y",
            "lastname": "Okada"
        },
        {
            "affiliation": "Joint Research Chair of Innovative Drug Discovery in Immunology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nResearch Division, Chugai Pharmaceutical Co. Ltd, Yokohama, Kanagawa, Japan.",
            "firstname": "Kunihiro",
            "initials": "K",
            "lastname": "Hattori"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan.\nDepartment of Advanced Clinical and Translational Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.",
            "firstname": "Masashi",
            "initials": "M",
            "lastname": "Narazaki"
        },
        {
            "affiliation": "Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan. kumanogo@imed3.med.osaka-u.ac.jp.\nDepartment of Immunopathology, World Premier International Research Center Initiative (WPI), Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan. kumanogo@imed3.med.osaka-u.ac.jp.\nIntegrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), Osaka University, Suita, Osaka, Japan. kumanogo@imed3.med.osaka-u.ac.jp.\nCenter for Infectious Diseases for Education and Research (CiDER), Osaka University, Suita, Osaka, Japan. kumanogo@imed3.med.osaka-u.ac.jp.\nJapan Agency for Medical Research and Development - Core Research for Evolutional Science and Technology (AMED-CREST), Osaka University, Suita, Osaka, Japan. kumanogo@imed3.med.osaka-u.ac.jp.\nCenter for Advanced Modalities and DDS (CAMaD), Osaka University, Suita, Osaka, Japan. kumanogo@imed3.med.osaka-u.ac.jp.",
            "firstname": "Atsushi",
            "initials": "A",
            "lastname": "Kumanogoh"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2025. The Author(s).",
    "doi": "10.1038/s41467-025-58550-7",
    "journal": "Nature communications",
    "keywords": [],
    "methods": null,
    "publication_date": "2025-04-25",
    "pubmed_id": "40274824\n24940954\n34260886\n15001782\n36257987\n32601182\n36138183\n37095375\n38207030\n38447573\n2161532\n8027554\n9378982\n12595502\n32238918\n34795676\n34594043\n37821442\n35858733\n33762327\n28150241\n26476454\n28373871\n36527200\n38459242\n30542206\n36408947\n33391289\n24914698\n33746988\n27601627\n32524751\n36920946\n31781107\n32446935\n23487025\n33859290\n35168946\n15096178\n14530365\n31922056\n10211889\n35106973\n35110332\n23586463\n24658644\n31819264",
    "results": null,
    "title": "Neutrophil single-cell analysis identifies a type II interferon-related subset for predicting relapse of autoimmune small vessel vasculitis.",
    "xml": "<Element 'PubmedArticle' at 0x788504e898a0>"
}