{
    "abstract": "Klebsiella pneumoniae is a Gram-negative bacterium that colonizes the gastrointestinal tract and nasopharynx with many being linked to nosocomial infections. Extended-spectrum \u03b2-lactamases (ESBL)-producing and carbapenem-resistant K. pneumoniae is recognized by the World Health Organization (WHO) as a critical public health concern. In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates. Genome comparison, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome-SNP-based phylogenetic analysis (wg-SNP) were used for in-depth molecular characterization. in silico typing was used to determine the resistance genes, virulence factors, Inc. groups, and capsular types. All except one isolate were non-susceptible to meropenem and 89% were non-susceptible to ertapenem and imipenem. bla",
    "authors": [
        {
            "affiliation": "Department of Natural Sciences, Lebanese American University, P.O. Box 36, Byblos, Lebanon.",
            "firstname": "Hassan",
            "initials": "H",
            "lastname": "Diab"
        },
        {
            "affiliation": "Department of Computer Science and Mathematics, Lebanese American University, P.O. Box 36, Byblos, Lebanon.",
            "firstname": "Kelven",
            "initials": "K",
            "lastname": "Rahy"
        },
        {
            "affiliation": "Clinical Laboratory Department, Makassed General Hospital, P.O.Box 11-6301, Beirut, Lebanon.",
            "firstname": "Tamima",
            "initials": "T",
            "lastname": "Jisr"
        },
        {
            "affiliation": "Faculty of Health Sciences, University of Balamand, P.O.Box: 55251, Beirut, Lebanon.",
            "firstname": "Mira",
            "initials": "M",
            "lastname": "El Chaar"
        },
        {
            "affiliation": "Middle East Institute of Health Hospital, Clinical Laboratory Department, P.O.Box 60-387, Bsalim, Lebanon.",
            "firstname": "Edmond",
            "initials": "E",
            "lastname": "Abboud"
        },
        {
            "affiliation": "Department of Natural Sciences, Lebanese American University, P.O. Box 36, Byblos, Lebanon. Electronic address: stokajian@lau.edu.lb.",
            "firstname": "Sima",
            "initials": "S",
            "lastname": "Tokajian"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2024 The Authors. Published by Elsevier B.V. All rights reserved.",
    "doi": "10.1016/j.meegid.2024.105583",
    "journal": "Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases",
    "keywords": [
        "Klebsiella pneumoniae",
        "Klebsiella variicola",
        "Klebsiellaquasipneumoniae",
        "NDM",
        "ST147"
    ],
    "methods": null,
    "publication_date": "2024-03-15",
    "pubmed_id": "38484958",
    "results": null,
    "title": "Phenotypic and molecular characterization of multi-drug resistant Klebsiella spp. isolates recovered from clinical settings.",
    "xml": "<Element 'PubmedArticle' at 0x7885034647c0>"
}