{
    "abstract": "Peripheral arterial diseases (PAD) have been reported to be the leading cause for limb amputations, and the current therapeutic strategies including antiplatelet medication or intervene surgery are reported to not clinically benefit the patients with high-grade PAD. To this respect, revascularization based on angiogenetic vascular endothelial growth factor (VEGF) gene therapy was attempted for the potential treatment of critical PAD. Aiming for transcellular delivery of VEGF-encoding plasmid DNA (pDNA), we proposed to elaborate intriguing virus-like DNA condensates, wherein the supercoiled rigid micrometer-scaled plasmid DNA (pDNA) could be regulated in an orderly fashion into well-defined nano-toroids by following a self-spooling process with the aid of cationic block copolymer poly(ethylene glycol)-polylysine at an extraordinary ionic strength (NaCl: 600 mM). Moreover, reversible disulfide crosslinking was proposed between the polylysine segments with the aim of stabilizing these intriguing toroidal condensates. Pertaining to the critical hindlimb ischemia, our proposed toroidal VEGF-encoding pDNA condensates demonstrated high levels of VEGF expression at the dosage sites, which consequently contributed to the neo-vasculature (the particularly abundant formation of micro-vessels in the injected hindlimb), preventing the hindlimb ischemia from causing necrosis at the extremities. Moreover, excellent safety profiles have been demonstrated by our proposed toroidal condensates, as opposed to the apparent immunogenicity of the naked pDNA. Hence, our proposed virus-like DNA condensates herald potentials as gene therapy platform in persistent expressions of the therapeutic proteins, and might consequently be highlighted in the management of a variety of intractable diseases.",
    "authors": [
        {
            "affiliation": "Department of Gastric Surgery, Cancer Hospital of China Medical University, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China.\nDepartment of Gastric Surgery, Cancer Hospital of Dalian University of Technology, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China.\nProvincial Key Laboratory of Interdisciplinary Medical Engineering for Gastrointestinal Carcinoma, Liaoning Cancer Hospital & Institute, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China.",
            "firstname": "Yue",
            "initials": "Y",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Materials Science and Engineering, Tsinghua University, Beijing City, 100084, China.\nInnovation Center of Yangtze River Delta, Zhejiang University, Jiaxing, Zhejiang, 314100, China.",
            "firstname": "Jun",
            "initials": "J",
            "lastname": "Liu"
        },
        {
            "affiliation": "Department of Vascular Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116023, China.",
            "firstname": "Changgui",
            "initials": "C",
            "lastname": "Tong"
        },
        {
            "affiliation": "Department of Vascular Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116023, China.",
            "firstname": "Lei",
            "initials": "L",
            "lastname": "Li"
        },
        {
            "affiliation": "Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing, Zhejiang, 314100, China.",
            "firstname": "Hongyang",
            "initials": "H",
            "lastname": "Cui"
        },
        {
            "affiliation": "Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing, Zhejiang, 314100, China.",
            "firstname": "Liuwei",
            "initials": "L",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Department of Gastric Surgery, Cancer Hospital of China Medical University, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China.\nDepartment of Gastric Surgery, Cancer Hospital of Dalian University of Technology, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China.",
            "firstname": "Ming",
            "initials": "M",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Department of Thyroid Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, 310022, China.\nHangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China.\nKey Laboratory of Head & Neck Cancer Translational Research of Zhejiang Province, Hangzhou, Zhejiang, 310022, China.\nPostgraduate Training Base Alliance of Wenzhou Medical University (Zhejiang Cancer Hospital), Hangzhou, Zhejiang, 310022, China.",
            "firstname": "Shijia",
            "initials": "S",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Department of Thyroid Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, 310022, China.\nHangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China.\nKey Laboratory of Head & Neck Cancer Translational Research of Zhejiang Province, Hangzhou, Zhejiang, 310022, China.\nPostgraduate Training Base Alliance of Wenzhou Medical University (Zhejiang Cancer Hospital), Hangzhou, Zhejiang, 310022, China.",
            "firstname": "Kehui",
            "initials": "K",
            "lastname": "Zhou"
        },
        {
            "affiliation": "Department of Thyroid Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, 310022, China. lanxb@zjcc.org.cn.\nHangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China. lanxb@zjcc.org.cn.\nKey Laboratory of Head & Neck Cancer Translational Research of Zhejiang Province, Hangzhou, Zhejiang, 310022, China. lanxb@zjcc.org.cn.\nPostgraduate Training Base Alliance of Wenzhou Medical University (Zhejiang Cancer Hospital), Hangzhou, Zhejiang, 310022, China. lanxb@zjcc.org.cn.",
            "firstname": "Xiabin",
            "initials": "X",
            "lastname": "Lan"
        },
        {
            "affiliation": "Provincial Key Laboratory of Interdisciplinary Medical Engineering for Gastrointestinal Carcinoma, Liaoning Cancer Hospital & Institute, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China. plasmid@zju.edu.cn.\nInnovation Center of Yangtze River Delta, Zhejiang University, Jiaxing, Zhejiang, 314100, China. plasmid@zju.edu.cn.",
            "firstname": "Qixian",
            "initials": "Q",
            "lastname": "Chen"
        },
        {
            "affiliation": "Department of Gastric Surgery, Cancer Hospital of China Medical University, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China. zhaoyan@cancerhosp-ln-cmu.com.\nDepartment of Gastric Surgery, Cancer Hospital of Dalian University of Technology, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China. zhaoyan@cancerhosp-ln-cmu.com.\nProvincial Key Laboratory of Interdisciplinary Medical Engineering for Gastrointestinal Carcinoma, Liaoning Cancer Hospital & Institute, No. 44 Xiaoheyan Road, Dadong District, Shenyang City, Liaoning, 110042, China. zhaoyan@cancerhosp-ln-cmu.com.",
            "firstname": "Yan",
            "initials": "Y",
            "lastname": "Zhao"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2024. The Author(s).",
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    "journal": "Journal of nanobiotechnology",
    "keywords": [
        "Angiogenesis",
        "DNA condensates",
        "Ischemia",
        "Vascular endothelial growth factors",
        "Virus-like gene delivery systems"
    ],
    "methods": null,
    "publication_date": "2024-07-15",
    "pubmed_id": "39004736\n32575692\n27853158\n28933520\n32772646\n29132879\n31621531\n24984839\n35203679\n29529399\n30621518\n21860391\n29065929\n34110899\n32634464\n9346244\n31057775\n26226080\n20712309\n31657906\n25433365\n32763187\n20196649\n25055856\n21993237\n26274378\n34445645\n35640491\n20043959\n20393508\n31805256",
    "results": null,
    "title": "Gene therapy by virus-like self-spooling toroidal DNA condensates for revascularization of hindlimb ischemia.",
    "xml": "<Element 'PubmedArticle' at 0x7885039038d0>"
}