{
    "abstract": "Adiponectin (AD) cardioprotective activities are mediated by AMPK, a fuel-sensing molecule sharing common targets and cellular activities with SIRT-1. Whether AD preconditioning may involve SIRT-1 activity is not known; however, the protective role of SIRT-1 during ischemia and the potential interplay between AMPK and SIRT-1 suggest this possibility.\nIsolated hearts from male Sprague-Dawley rats (n = 85) underwent ischemia/reperfusion (I/R, 30/180 min). Preconditioning with resveratrol (RSV, SIRT-1 activator) was compared to preconditioning with AD alone, or in combination with compound C (CC, AMPK inhibitor) or sirtinol (STN, SIRT-1 inhibitor). For each heart, left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (dLVP), coronary flow (CF) and left ventricular infarct mass (IM) were measured, together with the phosphorylation/activation status of AMPK, LKB1, eNOS and SIRT-1, at the beginning (15 min) and at the end (180 min) of reperfusion.\nWhen compared to I/R, both RSV and AD improved cardiac function and reduced IM (p < 0.01, p < 0.05, respectively). Cardioprotective effects of AD were completely reversed in the AD+CC group, and significantly attenuated in the AD+STN group. Both RSV and AD increased eNOS, AMPK and LKB1 phosphorylation (for each parameter: p < 0.05 vs. I/R, in both RSV and AD treatment groups) at 15 min of reperfusion, and SIRT-1 activity at the end of reperfusion (p < 0.01, p < 0.05 vs. I/R, respectively). Interestingly, AD-mediated phosphorylation of AMPK and LKB1, and SIRT-1 deacetylation activity was markedly reduced in both the AD+CC and AD+STN groups (p < 0.05 vs. AD). Thus, AD-mediated cardioprotection requires both AMPK and SIRT-1 signaling pathways, that act as a component of a cycle and regulate each other's activities.",
    "authors": [
        {
            "affiliation": "Department of Biomedical Sciences and Human Oncology-Pharmacology Section, Medical School-University of Bari \"Aldo Moro\", Bari, Italy.",
            "firstname": "Maria Assunta",
            "initials": "MA",
            "lastname": "Potenza"
        },
        {
            "affiliation": "Department of Emergency and Organ Transplantation-Section of Cardiovascular Diseases, Medical School-University of Bari \"Aldo Moro\", Bari, Italy.",
            "firstname": "Luca",
            "initials": "L",
            "lastname": "Sgarra"
        },
        {
            "affiliation": "Department of Biomedical Sciences and Human Oncology-Pharmacology Section, Medical School-University of Bari \"Aldo Moro\", Bari, Italy.",
            "firstname": "Carmela",
            "initials": "C",
            "lastname": "Nacci"
        },
        {
            "affiliation": "Department of Biomedical Sciences and Human Oncology-Pharmacology Section, Medical School-University of Bari \"Aldo Moro\", Bari, Italy.",
            "firstname": "Valentina",
            "initials": "V",
            "lastname": "Leo"
        },
        {
            "affiliation": "Department of Biomedical Sciences and Human Oncology-Pharmacology Section, Medical School-University of Bari \"Aldo Moro\", Bari, Italy.",
            "firstname": "Maria Antonietta",
            "initials": "MA",
            "lastname": "De Salvia"
        },
        {
            "affiliation": "Department of Biomedical Sciences and Human Oncology-Pharmacology Section, Medical School-University of Bari \"Aldo Moro\", Bari, Italy.",
            "firstname": "Monica",
            "initials": "M",
            "lastname": "Montagnani"
        }
    ],
    "conclusions": null,
    "copyrights": null,
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    "journal": "PloS one",
    "keywords": [],
    "methods": null,
    "publication_date": "2019-01-18",
    "pubmed_id": "30653603\n26843289\n25261534\n18991672\n7645523\n26160707\n10693811\n19276888\n19491041\n18805010\n15205477\n19262508\n23259556\n29213353\n16380484\n15314686\n17337600\n10025949\n12433878\n10903999\n18550784\n15604409\n18555842\n18687677\n15486319\n17446436\n21060073\n20823277\n21856913\n29409011\n12524229\n16651465\n15082700\n17343838\n21900123\n12944390\n14551684\n23173099\n18222959\n21399860\n16155579\n24520397\n26585840\n24327606\n22046359\n22854413\n942051\n8593703\n16344382\n26058828\n18339648\n17664136\n20008278\n24535859\n17109977\n18083782\n19188503\n15159410\n25863291\n19107194\n18477450\n16039971\n18482975\n12748233\n16466346",
    "results": null,
    "title": "Activation of AMPK/SIRT1 axis is required for adiponectin-mediated preconditioning on myocardial ischemia-reperfusion (I/R) injury in rats.",
    "xml": "<Element 'PubmedArticle' at 0x788503cbcc20>"
}