{
    "abstract": "Osteosarcoma is the most common primary bone sarcoma with a bimodal age distribution. Complete surgical resection with neoadjuvant chemotherapy is the standard curative treatment, and no effective therapeutic strategy has been established for metastatic cases, resulting in poor prognosis. Osteosarcoma presents complex and heterogeneous clinical and molecular features, and no molecular-targeted drugs are available. Therefore, effective multidisciplinary treatment strategies are urgently required. Patient-derived cell lines are essential tools in basic and translational oncology. Considering the heterogeneity of the disease, we established a novel cell line, NCC-OS2-C1, using surgically resected tumor tissues from a patient with osteosarcoma. NCC-OS2-C1 cells demonstrated constant proliferation, spheroid formation, and invasion. In addition, we demonstrated that NCC-OS2-C1 is applicable for the high-throughput screening of drugs. Thus, NCC-OS2-C1 is a valuable tool for basic and translational research on osteosarcoma.",
    "authors": [
        {
            "affiliation": "Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.\nDepartment of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba, 260-8670, Japan.",
            "firstname": "Shuhei",
            "initials": "S",
            "lastname": "Iwata"
        },
        {
            "affiliation": "Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Rei",
            "initials": "R",
            "lastname": "Noguchi"
        },
        {
            "affiliation": "Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Julia",
            "initials": "J",
            "lastname": "Osaki"
        },
        {
            "affiliation": "Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Yuki",
            "initials": "Y",
            "lastname": "Adachi"
        },
        {
            "affiliation": "Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Yomogi",
            "initials": "Y",
            "lastname": "Shiota"
        },
        {
            "affiliation": "Department of Musculoskeletal Oncology and Rehabilitation Medicine, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Koichi",
            "initials": "K",
            "lastname": "Ogura"
        },
        {
            "affiliation": "Department of Diagnostic Pathology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Shogo",
            "initials": "S",
            "lastname": "Nishino"
        },
        {
            "affiliation": "Department of Diagnostic Pathology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Akihiko",
            "initials": "A",
            "lastname": "Yoshida"
        },
        {
            "affiliation": "Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba, 260-8670, Japan.",
            "firstname": "Seiji",
            "initials": "S",
            "lastname": "Ohtori"
        },
        {
            "affiliation": "Department of Musculoskeletal Oncology and Rehabilitation Medicine, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan.",
            "firstname": "Akira",
            "initials": "A",
            "lastname": "Kawai"
        },
        {
            "affiliation": "Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-Ku, Tokyo, 104-0045, Japan. proteomebioinformatics@gmail.com.",
            "firstname": "Tadashi",
            "initials": "T",
            "lastname": "Kondo"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2025. The Author(s) under exclusive licence to Japan Human Cell Society.",
    "doi": "10.1007/s13577-025-01198-8\n10.1186/s12938-021-00860-0\n10.1177/1947601911413466\n10.1073/pnas.82.18.6216\n10.1186/1750-1172-5-2\n10.1016/j.annonc.2021.08.1995\n10.1016/j.anndiagpath.2020.151654\n10.1177/10732748241274188\n10.3390/cells11213507\n10.1080/10717544.2024.2391001\n10.1016/j.ctarc.2021.100324\n10.1016/j.bone.2019.115070\n10.7171/jbt.18-2902-002\n10.1038/s41598-023-47412-1\n10.1016/j.bone.2014.02.003\n10.1038/nrc3838\n10.1634/theoncologist.9-4-422\n10.1016/j.pharmthera.2012.09.003\n10.1038/nrd.2016.175\n10.1007/s11626-018-0274-2",
    "journal": "Human cell",
    "keywords": [
        "Bone sarcoma",
        "Cell line",
        "Drug screening",
        "Osteosarcoma",
        "Patient-derived cancer model"
    ],
    "methods": null,
    "publication_date": "2025-04-02",
    "pubmed_id": "40169461\n33653371\n21779515\n2994066\n20113479\n34500044\n33130384\n39183728\n36359903\n39239763\n31525475\n29805321\n37978271\n24530473\n25319867\n15266096\n22983152\n27616293",
    "results": null,
    "title": "Establishment and characterization of NCC-OS2-C1: a novel patient-derived cell line of osteosarcoma.",
    "xml": "<Element 'PubmedArticle' at 0x788506499fd0>"
}