{
    "abstract": "The study of natural glycans is fundamental to modern glycomics; however, the functional analysis of these glycans is limited by their low natural abundance, structural heterogeneity, and the absence of efficient preparative-scale isolation methods. Here, we present a streamlined approach for the gram-scale production of complex natural N-glycans in their reducing form by combining oxidative release of natural glycans (ORNG) using household bleach, an innovative cleavable tag chemistry, and optimized multi-dimensional chromatography. Our ORNG process releases N-glycans from kilogram-scale of natural sources containing various glycoproteins, which can be efficiently attached with a 4-aminobenzoate tag, facilitating the selective separation of these glycans from other biomolecules. The tagged glycans can be purified via dry-load HILIC flash chromatography at gram scale followed by reverse-phase HPLC. Treatment with Oxone quantitatively cleaves the tag, regenerating the free reducing N-glycans in high yield. This method provides an accessible, gram-scale source of complex N-glycans, including complex asymmetric structures that are challenging to synthesize through conventional chemoenzymatic approaches. We believe this approach represents a versatile strategy for acquiring complex natural glycans, opening new avenues for the functional exploration of N-glycans in glycoscience.",
    "authors": [
        {
            "affiliation": "Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.",
            "firstname": "Qing",
            "initials": "Q",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Emory Glycomics and Molecular Interactions Core, Emory University School of Medicine, Atlanta, GA, USA.",
            "firstname": "Yi",
            "initials": "Y",
            "lastname": "Lasanajak"
        },
        {
            "affiliation": "NatGlycan LLC, Tucker, GA, USA.",
            "firstname": "Menxin",
            "initials": "M",
            "lastname": "Yan"
        },
        {
            "affiliation": "NatGlycan LLC, Tucker, GA, USA.",
            "firstname": "Golden",
            "initials": "G",
            "lastname": "Chen"
        },
        {
            "affiliation": "Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA. xsong2@emory.edu.\nEmory Glycomics and Molecular Interactions Core, Emory University School of Medicine, Atlanta, GA, USA. xsong2@emory.edu.",
            "firstname": "Xuezheng",
            "initials": "X",
            "lastname": "Song"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2025. The Author(s).",
    "doi": "10.1038/s42004-025-01499-x",
    "journal": "Communications chemistry",
    "keywords": [],
    "methods": null,
    "publication_date": "2025-04-05",
    "pubmed_id": "40185879\n21111227\n11701646\n15217604\n16959566\n15459677\n18492092\n23287290\n18194658\n19344114\n20225063\n21983600\n20690605\n19181540\n17223647\n17384119\n19454239\n18804025\n37259796\n37235553\n17013397\n19171304\n21469953\n17922555\n15563589\n21131969\n23888036\n26417422\n30792508\n28396442\n29732660\n27135973\n29803109\n39387489\n21145794\n16278656\n762044\n7503412\n8900951\n21803123\n16278655\n26079702\n33891963\n23992636\n35143205\n35569240\n7954510\n25924827\n28190870\n7733678\n10833030\n6826553\n7142137\n6825842",
    "results": null,
    "title": "Streamlined gram-scale natural N-glycan production using reversible tagging after oxidative release of natural glycans.",
    "xml": "<Element 'PubmedArticle' at 0x788503de5e40>"
}