{
    "abstract": "Right heart failure is the terminal stage of PAH. When PAH patients suffer from pulmonary infection or puerperal infection heart failure often rapidly develops. Low dose of lipopolysaccharide induces rapid right ventricular failure in rats with pulmonary arterial hypertension.\nThe objective of this study was to investigate whether the NLRP3 inflammasome mediates disturbance of the ventricular immune microenvironment of PAH rats and promotes right ventricular failure.\nIntraperitoneal injection of monocrotaline was used to induce PAH in rats. Right ventricular function was measured via echocardiography before and after the rats were treated with lipopolysaccharide and MCC950. The degree of immune microenvironment disturbance in right ventricular tissue was measured with a rat chemokine and cytokine antibody array, Western blot, flow cytometry and quantitative real-time PCR analysis.\nAfter the rats were injected with LPS, they exhibited right ventricular dysfunction and a significant increase in right ventricular tissue inflammation with elevated M1 macrophage proportion. Administration of MCC950 suppressed inflammation and improved right ventricular function. The number of M1 macrophages was decreased after MCC950 treatment. NLRP3 inflammasome inhibition ameliorated LPS-induced changes in the immune microenvironment in the right heart and right ventricular dysfunction in rats with PAH.\nSelective inhibition of NLRP3 pathway interfered the interaction between hypertrophic cardiomyocytes and macrophages in the initial stage of inflammation and maintained the immune microenvironment balance, eventually contributing to attenuation of LPS-induced acute heart failure in PAH rats.",
    "authors": [
        {
            "affiliation": "Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, People's Republic of China.",
            "firstname": "Lizhe",
            "initials": "L",
            "lastname": "Guo"
        },
        {
            "affiliation": "Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, People's Republic of China.",
            "firstname": "Gang",
            "initials": "G",
            "lastname": "Qin"
        },
        {
            "affiliation": "Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, People's Republic of China.",
            "firstname": "Yanan",
            "initials": "Y",
            "lastname": "Cao"
        },
        {
            "affiliation": "Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, People's Republic of China.",
            "firstname": "Yue",
            "initials": "Y",
            "lastname": "Yang"
        },
        {
            "affiliation": "Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, People's Republic of China.",
            "firstname": "Sisi",
            "initials": "S",
            "lastname": "Dai"
        },
        {
            "affiliation": "Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, People's Republic of China.",
            "firstname": "Lu",
            "initials": "L",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, People's Republic of China.\nNational Clinical Research Center for Geriatric Disorders (Xiangya Hospital), Changsha, People's Republic of China.",
            "firstname": "E",
            "initials": "E",
            "lastname": "Wang"
        }
    ],
    "conclusions": "Selective inhibition of NLRP3 pathway interfered the interaction between hypertrophic cardiomyocytes and macrophages in the initial stage of inflammation and maintained the immune microenvironment balance, eventually contributing to attenuation of LPS-induced acute heart failure in PAH rats.",
    "copyrights": "\u00a9 2021 Guo et al.",
    "doi": "10.2147/JIR.S336964\n10.1177/2045893217714463\n10.1177/2045893217744504\n10.1016/j.healun.2013.10.015\n10.1093/cvr/cvx159\n10.1038/nri3452\n10.1074/jbc.M203915200\n10.1016/s1097-2765(02)00599-3\n10.3390/ijms22031064\n10.1002/med.21781\n10.1093/cvr/cvt091\n10.1093/cvr/cvu259\n10.1186/s13578-020-00434-y\n10.1046/j.1365-2958.2000.02103.x\n10.1002/1521-4141(200205)32:5<1464::Aid-immu1464>3.0.Co;2-g\n10.1111/jpi.12449\n10.1038/mi.2016.73\n10.1155/2017/9743280\n10.1016/j.bbrc.2018.03.005\n10.3390/cancers12010193\n10.1016/j.tox.2018.09.002\n10.1111/jcmm.13743\n10.1016/j.redox.2019.101215\n10.18632/oncotarget.17256\n10.3389/fmicb.2019.02020\n10.1038/s41401-019-0305-x\n10.1038/s41401-019-0305-x\n10.1038/s41591-018-0059-x\n10.1007/s11095-017-2277-1\n10.1530/joe-19-0190\n10.1021/acs.molpharmaceut.8b00237\n10.1172/jci.insight.126832\n10.1016/j.lfs.2020.118808\n10.1183/13993003.02308-2018\n10.1164/rccm.201406-1137OC\n10.4049/jimmunol.1302821\n10.1111/jcmm.14984\n10.1161/circresaha.118.314028\n10.1002/eji.201948414\n10.2337/db18-1231\n10.1172/jci123778\n10.1016/j.carpath.2016.05.006\n10.1111/j.1365-2613.2008.00610.x\n10.1161/circresaha.118.314030",
    "journal": "Journal of inflammation research",
    "keywords": [
        "NLRP3 inflammasome",
        "immune microenvironment",
        "inflammation",
        "pulmonary arterial hypertension",
        "right ventricular failure"
    ],
    "methods": null,
    "publication_date": "2021-11-11",
    "pubmed_id": "34754216\n28628000\n29251561\n24268184\n28957536\n23702978\n12019269\n12191486\n33494430\n33460162\n23580606\n25524927\n32489586\n11029688\n11981835\n29024030\n27554297\n29062465\n29510138\n31941010\n30205151\n29993180\n31121492\n28489580\n31551961\n31530901\n28167322\n29892064\n29305668\n31137011\n29791797\n31536479\n33245967\n31320454\n25536148\n24639350\n31957197\n30582448\n32068249\n31439648\n31169521\n27327107\n18808531\n30661445",
    "results": "After the rats were injected with LPS, they exhibited right ventricular dysfunction and a significant increase in right ventricular tissue inflammation with elevated M1 macrophage proportion. Administration of MCC950 suppressed inflammation and improved right ventricular function. The number of M1 macrophages was decreased after MCC950 treatment. NLRP3 inflammasome inhibition ameliorated LPS-induced changes in the immune microenvironment in the right heart and right ventricular dysfunction in rats with PAH.",
    "title": "Regulation of the Immune Microenvironment by an NLRP3 Inhibitor Contributes to Attenuation of Acute Right Ventricular Failure in Rats with Pulmonary Arterial Hypertension.",
    "xml": "<Element 'PubmedArticle' at 0x7885025711c0>"
}