{
    "abstract": "Therapeutic strategies for cerebral ischemia/reperfusion (I/R) injury, an important contributor to neurological impairment and disability, exhibit limited efficacy. Reperfusion therapy intensifies neuronal damage by promoting iron deposition, ferroptosis (lipid peroxidation-associated iron-dependent cellular death), and reactive oxygen species (ROS) accumulation.\nwe investigated the role of the m6A demethylase FTO in modulating ferroptosis during cerebral I/R injury, using middle cerebral artery occlusion/reperfusion (MCAO/R) model rats and neuronal cells subjected to oxygen glucose deprivation/reoxygenation (OGD/R) as in vivo and in vitro experimental platforms, respectively. Neurological scores and cerebral infarction volumes were measured by TTC staining. FTO, OTUB1, and SLC7A11 levels, and FTO demethylase activity, were assessed by qRT-PCR, western blotting, and immunohistochemistry. MeRIP was applied to ascertain the m6A methylation status of OTUB1 mRNA. Apoptotic rates and cell viability were quantitatively aalyzed by flow cytometry and CCK-8 assay, respectively, while brain tissue apoptosis was evaluated using TUNEL staining.\nMCAO/R rat brains and OGD/R cells showed decreased FTO expression and increased OTUB1 m6A methylation. FTO overexpression upregulated OTUB1 by diminishing m6A methylation, consequently stabilizing SLC7A11 and reducing ferroptosis. FTO or OTUB1 silencing increased ferroptosis, while their co-overexpression enhanced neuroprotective effects. FTO overexpression reduced infarct volume and cell apoptosis, and improved neurological outcomes in vivo.\nFTO enhanced OTUB1 expression via m6A demethylation, stabilizing SLC7A11, and inhibiting ferroptosis to alleviate cerebral I/R injury. The FTO/OTUB1/SLC7A11 pathway is a viable therapeutic target for ischemic stroke, providing novel perspectives on the molecular mechanisms underlying neuroprotection and proposing innovative m6A-based therapies.",
    "authors": [
        {
            "affiliation": "The Second School of Clinical Medicine, Southern Medical University, Guangzhou City, Guangdong Province, PR China; Department of Neurology, Deqing People's Hospital, Zhaoqing City, Guangdong Province, PR China.",
            "firstname": "Youjin",
            "initials": "Y",
            "lastname": "Shen"
        },
        {
            "affiliation": "The Second School of Clinical Medicine, Southern Medical University, Guangzhou City, Guangdong Province, PR China; Department of Emergency, Huhhot First Hospital, Huhhot City, Inner Mongolia Autonomous Region, PR China.",
            "firstname": "Wentao",
            "initials": "W",
            "lastname": "Liu"
        },
        {
            "affiliation": "Department of Neurology, Deqing People's Hospital, Zhaoqing City, Guangdong Province, PR China.",
            "firstname": "Zonghua",
            "initials": "Z",
            "lastname": "Zhou"
        },
        {
            "affiliation": "Department of Neurology, Deqing People's Hospital, Zhaoqing City, Guangdong Province, PR China.",
            "firstname": "Jianwen",
            "initials": "J",
            "lastname": "He"
        },
        {
            "affiliation": "The Second School of Clinical Medicine, Southern Medical University, Guangzhou City, Guangdong Province, PR China; Department of Neurology, The Sixth Medical Center of PLA of Chinese General Hospital, Beijing, PR China. Electronic address: bjqxk@sina.com.",
            "firstname": "Xiaokun",
            "initials": "X",
            "lastname": "Qi"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2025. Published by Elsevier Inc.",
    "doi": "10.1016/j.jstrokecerebrovasdis.2025.108316",
    "journal": "Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association",
    "keywords": [
        "Cerebral I/R injury",
        "FTO",
        "Ferroptosis",
        "OTUB1",
        "SLC7A11",
        "m6A demethylation"
    ],
    "methods": null,
    "publication_date": "2025-04-16",
    "pubmed_id": "40233842",
    "results": "MCAO/R rat brains and OGD/R cells showed decreased FTO expression and increased OTUB1 m6A methylation. FTO overexpression upregulated OTUB1 by diminishing m6A methylation, consequently stabilizing SLC7A11 and reducing ferroptosis. FTO or OTUB1 silencing increased ferroptosis, while their co-overexpression enhanced neuroprotective effects. FTO overexpression reduced infarct volume and cell apoptosis, and improved neurological outcomes in vivo.",
    "title": "FTO-mediated m6A Demethylation of OTUB1 stabilizes SLC7A11 to alleviate Ferroptosis in cerebral ischemia/reperfusion injury.",
    "xml": "<Element 'PubmedArticle' at 0x788504d0f8d0>"
}