{
    "abstract": "Neutrophil Extracellular Traps (NETs) are closely related to the progression of inflammation in Chronic Obstructive Pulmonary Disease (COPD). Erythromycin (EM) has been shown to inhibit inflammation in COPD, but its molecular mechanisms is still unclear. The aim of our study is investigate the molecular mechanisms of EM's anti-inflammatory effects in NETs-induced inflammation.\nTranscriptomics and proteomics data were obtained from U937 cells treated with NETs and EM. Differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were identified using R software. Pathway enrichment analyses, were employed to identify inflammation-related pathways. Cytoscape were utilized to construct network of hub targets regulated by EM which related with oxidative stress and inflammation. Additionally, Cytoscape and STRING were used to construct protein-protein interaction (PPI) network of key targets regulated by EM. The expression levels of key targets were further confirmed through WB and PCR experiments.\nBoth transcriptomics and proteomics indicate that EM decrease NETs -induced AKT1 expression. Enrichment analysis of DEGs and DEPs reveal multiple common pathways involved in EM's regulation inflammation, including the PI3K/AKT pathway, response to oxidative stress, IKK/NF-\u03baB signaling and PTEN signaling pathway. Nine key targets in PI3K/AKT-related inflammatory pathways regulated by EM and ten targets of EM-regulated oxidative stress were identified. WB and PCR results confirmed that EM reversing the NETs-induced inflammation by modulating the activity of these targets. Furthermore, clinical samples and vitro experiments confirm that EM alleviates NETs-induced glucocorticoid resistance via inhibiting PI3K/AKT, thereby repressing inflammation.\nOur study provides a comprehensive proteogenomic characterization of how EM alleviates NET-related inflammation, and identify PI3K/AKT play a critical role in the mechanism by which EM inhibits inflammation.",
    "authors": [
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Nan",
            "initials": "N",
            "lastname": "Ma"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Xiao Na",
            "initials": "XN",
            "lastname": "Liang"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Quan Fang",
            "initials": "QF",
            "lastname": "Chen"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Mei Hua",
            "initials": "MH",
            "lastname": "Li"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Guang Sheng",
            "initials": "GS",
            "lastname": "Pei"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Xiao Fei",
            "initials": "XF",
            "lastname": "Yi"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Li Yan",
            "initials": "LY",
            "lastname": "Guo"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.",
            "firstname": "Fu Gang",
            "initials": "FG",
            "lastname": "Chen"
        },
        {
            "affiliation": "Department of Respiratory Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. zhiyi-river@163.com.",
            "firstname": "Zhi Yi",
            "initials": "ZY",
            "lastname": "He"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2025. The Author(s).",
    "doi": "10.1186/s12931-025-03226-5",
    "journal": "Respiratory research",
    "keywords": [
        "Erythromycin",
        "Inflammation",
        "NETs",
        "PI3K/AKT",
        "Proteogenomic"
    ],
    "methods": null,
    "publication_date": "2025-04-20",
    "pubmed_id": "40253327\n36858443\n38929978\n39000454\n38672169\n38283037\n30262472\n36495695\n31515489\n30804927\n31265835\n35197267\n28720648\n26804470\n38880789\n29568356\n22265969\n23817204\n29427576\n27350308\n22830464\n29543980\n22925316\n20881376\n31242482\n25957293\n31770582\n23652462\n32104150\n34707493\n36506578\n38880789\n37987263\n37348121\n33609487\n32829090\n39585643\n23360759\n37799975\n34916492\n35740927\n27959617\n38191199\n36401729\n33188176\n26113679\n31655606\n19850036\n26320152\n33554439\n33252860\n24831826\n19519318\n38456508\n9690619\n35044626\n37852657\n36413806\n31172493\n38796028\n37949302\n28522564\n25763354\n30999672\n35657152\n22435559\n15371334\n18620044\n34181887\n34012501\n34658913\n29499228\n37399718\n25196599\n36333711\n32912335\n29373533\n39453846\n24569375\n26024894\n14691478\n37237941\n32215168\n39138434\n23542362\n17041012",
    "results": "Both transcriptomics and proteomics indicate that EM decrease NETs -induced AKT1 expression. Enrichment analysis of DEGs and DEPs reveal multiple common pathways involved in EM's regulation inflammation, including the PI3K/AKT pathway, response to oxidative stress, IKK/NF-\u03baB signaling and PTEN signaling pathway. Nine key targets in PI3K/AKT-related inflammatory pathways regulated by EM and ten targets of EM-regulated oxidative stress were identified. WB and PCR results confirmed that EM reversing the NETs-induced inflammation by modulating the activity of these targets. Furthermore, clinical samples and vitro experiments confirm that EM alleviates NETs-induced glucocorticoid resistance via inhibiting PI3K/AKT, thereby repressing inflammation.",
    "title": "Proteogenomic verifies targets underlying erythromycin alleviate neutrophil extracellular traps-induced inflammation.",
    "xml": "<Element 'PubmedArticle' at 0x788505523920>"
}