{
    "abstract": "The freeze-thaw process can induce irreversible structural and functional changes in human sperm, particularly sperm DNA damage. Selecting a more accurate and sensitive detection method for evaluating sperm DNA integrity is crucial. To accurately assess sperm DNA integrity following the freeze-thaw process and significantly improve the clinical and scientific utilization of cryopreserved sperm. In this study, we utilized a novel fluorescent biosensor, assisted by terminal deoxynucleotidyl transferase (TdT) and Endonuclease IV, to detect DNA breakpoints during sperm cryopreservation. We evaluated the biosensor's performance by comparing it with the conventional DNA fragmentation index (DFI) measured using sperm chromatin structure analysis (SCSA). The cryopreserved group exhibited a significantly higher sperm DFI compared to the fresh group. No significant difference was observed between the antioxidant group and the cryopreserved group. However, the new method revealed a significant reduction in the number of DNA breakpoints in the antioxidant group compared to the cryopreserved group. The novel biosensor demonstrated superior accuracy and effectiveness in assessing sperm DNA integrity during cryopreservation compared to the conventional SCSA method. We believe that the biosensor holds significant potential for widespread use in the field of reproductive medicine.",
    "authors": [
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China.",
            "firstname": "Yue",
            "initials": "Y",
            "lastname": "Zhou"
        },
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China; Department of Pathology, Ningxia Medical University, Yinchuan, 750001, PR China.",
            "firstname": "Juan",
            "initials": "J",
            "lastname": "Wang"
        },
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China.",
            "firstname": "Lifan",
            "initials": "L",
            "lastname": "Zhuo"
        },
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China.",
            "firstname": "Chengbin",
            "initials": "C",
            "lastname": "Pei"
        },
        {
            "affiliation": "Reproductive Center, General Hospital of Ningxia Medical University, Yinchuan, 750004, PR China.",
            "firstname": "Shaotong",
            "initials": "S",
            "lastname": "Jia"
        },
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China.",
            "firstname": "Jia",
            "initials": "J",
            "lastname": "Tian"
        },
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China.",
            "firstname": "Honghong",
            "initials": "H",
            "lastname": "Wang"
        },
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China. Electronic address: d202081630@hust.edu.cn.",
            "firstname": "Bei",
            "initials": "B",
            "lastname": "Yan"
        },
        {
            "affiliation": "Ningxia Human Sperm Bank, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan, 750004, PR China. Electronic address: mmm1770@163.com.",
            "firstname": "Lianghong",
            "initials": "L",
            "lastname": "Ma"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2023 Elsevier Inc. All rights reserved.",
    "doi": "10.1016/j.cryobiol.2023.104591",
    "journal": "Cryobiology",
    "keywords": [
        "DFI",
        "DNA breakpoint",
        "Endo IV",
        "ROS",
        "Sperm freezing",
        "TDT enzyme",
        "TdT/Endo IV Fluo-biosensor"
    ],
    "methods": null,
    "publication_date": "2023-10-08",
    "pubmed_id": "37804950",
    "results": null,
    "title": "Fluorescent enzyme-based biosensor for sensitive analysis of DNA damage in cryopreserved sperm.",
    "xml": "<Element 'PubmedArticle' at 0x788502aa1850>"
}