{
    "abstract": "Aleurone, derived from the bran layer of grains like wheat and barley, has demonstrated positive effects on energy metabolism in pigs, mice, and untrained horses, influencing glucose-insulin dynamics and gut microbiome composition. Training itself enhances insulin sensitivity in horses, similar to the improvements in performance capacity observed in human athletes. This study aimed to investigate whether aleurone supplementation provides additional benefits to training by modulating insulin metabolism and gut microbiota in Standardbred mares.\nSixteen Standardbred mares (aged 3-5 years) participated in a cross-over study with two 8-week training periods separated by 8 weeks of detraining. Each horse received either 200 g/day aleurone supplementation or a control diet. Insulin metabolism was evaluated using oral (OGTT) and intravenous (FSIGTT) glucose tolerance tests, measuring parameters such as Maximum\nTraining alone: Training significantly improved OGTT parameters by decreasing Maximum\nAleurone supplementation enhances training-induced improvements in glucose metabolism and fecal microbiota composition, which could offer potential benefits for equine athletes by optimizing metabolic flexibility. It also supports improvements in glucose and insulin dynamics, particularly by further enhancing insulin sensitivity and glucose-mediated disposal. Future studies should investigate the mechanisms of aleurone at the muscle and gut level and explore its potential applications for metabolic disorders such as Equine Metabolic Syndrome.",
    "authors": [
        {
            "affiliation": "Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.\nEquine Hospital Wolvega, Oldeholtpade, Netherlands.",
            "firstname": "Berit",
            "initials": "B",
            "lastname": "Boshuizen"
        },
        {
            "affiliation": "Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Lorie",
            "initials": "L",
            "lastname": "De Mar\u00e9"
        },
        {
            "affiliation": "Department of Large Animal Surgery, Anaesthesia and Orthopaedics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Maarten",
            "initials": "M",
            "lastname": "Oosterlinck"
        },
        {
            "affiliation": "Department of Pathobiology, Pharmacology and Special Animals Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Filip",
            "initials": "F",
            "lastname": "Van Immerseel"
        },
        {
            "affiliation": "Department of Pathobiology, Pharmacology and Special Animals Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Venessa",
            "initials": "V",
            "lastname": "Eeckhaut"
        },
        {
            "affiliation": "Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Constance",
            "initials": "C",
            "lastname": "De Meeus"
        },
        {
            "affiliation": "Gut-Liver ImmunoPharmacology Unit, Department of Basic and Applied Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.",
            "firstname": "Lindsey",
            "initials": "L",
            "lastname": "Devisscher"
        },
        {
            "affiliation": "Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Carmen",
            "initials": "C",
            "lastname": "Vidal Moreno de Vega"
        },
        {
            "affiliation": "Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Maarten",
            "initials": "M",
            "lastname": "Willems"
        },
        {
            "affiliation": "Cargill Research and Development Centre Europe, Vilvoorde, Belgium.",
            "firstname": "Jean Eduardo",
            "initials": "JE",
            "lastname": "De Oliveira"
        },
        {
            "affiliation": "Cargill Research and Development Centre Europe, Vilvoorde, Belgium.",
            "firstname": "Guilherme",
            "initials": "G",
            "lastname": "Hosotani"
        },
        {
            "affiliation": "Department of Pharmaceutics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.",
            "firstname": "Yannick",
            "initials": "Y",
            "lastname": "Gansemans"
        },
        {
            "affiliation": "Department of Pharmaceutics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.",
            "firstname": "Tim",
            "initials": "T",
            "lastname": "Meese"
        },
        {
            "affiliation": "Department of Pharmaceutics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.",
            "firstname": "Filip",
            "initials": "F",
            "lastname": "Van Nieuwerburgh"
        },
        {
            "affiliation": "Department of Pharmaceutics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.",
            "firstname": "Dieter",
            "initials": "D",
            "lastname": "Deforce"
        },
        {
            "affiliation": "Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.",
            "firstname": "Katrien",
            "initials": "K",
            "lastname": "Vanderperren"
        },
        {
            "affiliation": "Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.\nEquine Health and Performance Centre, School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Adelaide, SA, Australia.",
            "firstname": "Elisabeth-Lidwien",
            "initials": "EL",
            "lastname": "Verdegaal"
        },
        {
            "affiliation": "Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.\nEquine Health and Performance Centre, School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Adelaide, SA, Australia.",
            "firstname": "Cath\u00e9rine",
            "initials": "C",
            "lastname": "Delesalle"
        }
    ],
    "conclusions": "Aleurone supplementation enhances training-induced improvements in glucose metabolism and fecal microbiota composition, which could offer potential benefits for equine athletes by optimizing metabolic flexibility. It also supports improvements in glucose and insulin dynamics, particularly by further enhancing insulin sensitivity and glucose-mediated disposal. Future studies should investigate the mechanisms of aleurone at the muscle and gut level and explore its potential applications for metabolic disorders such as Equine Metabolic Syndrome.",
    "copyrights": "Copyright \u00a9 2025 Boshuizen, De Mar\u00e9, Oosterlinck, Van Immerseel, Eeckhaut, De Meeus, Devisscher, Vidal Moreno de Vega, Willems, De Oliveira, Hosotani, Gansemans, Meese, Van Nieuwerburgh, Deforce, Vanderperren, Verdegaal and Delesalle.",
    "doi": "10.3389/fphys.2025.1565005\n10.1007/s00535-015-1099-3\n10.1016/j.jevs.2019.02.006\n10.1016/j.tvjl.2007.07.003\n10.56808/2985-1130.2895\n10.3389/fvets.2018.00225\n10.1038/s41587-019-0209-9\n10.3390/ijerph\n10.1007/s004210050618\n10.3389/fvets.2021.642809\n10.1089/152091503322641060\n10.3390/nu15061534\n10.1080/10408398.2011.589540\n10.1038/nmeth.f.303\n10.2460/ajvr.71.3.314\n10.1093/nar/gkz862\n10.1038/s41467-020-17840-y\n10.1136/gutjnl-2013-306541\n10.1016/j.dld.2017.11.016\n10.1038/icb.2015.108\n10.1186/s12917-015-0335-7\n10.1017/S1466252312000035\n10.1016/j.numecd.2021.12.016\n10.1098/rstb.2022.0221\n10.1017/S0007114511003825\n10.1152/ajpendo.00362.2015\n10.3389/fphys.2022.792052\n10.1371/journal.pone.0249922\n10.1128/AEM.03006-05\n10.2337/diacare.26.3.557\n10.1111/jvim.15423\n10.1111/j.2042-3306.1989.tb02085.x\n10.1186/s40168-016-0189-7\n10.1371/journal.pone.0092193\n10.1007/s00394-022-02836-9\n10.3920/CEP200008\n10.1242/jeb.219154\n10.1111/eve.12130\n10.1038/s41564-018-0306-4\n10.1055/s-2003-38385\n10.1371/journal.pcbi.1002624\n10.3390/ANI11020290\n10.1111/j.2042-3306.1983.tb01826.x\n10.1080/10618600.1996.10474713\n10.3920/CEP160015\n10.3920/CEP160030\n10.1111/j.2042-3306.1986.tb03556.x\n10.1186/1750-1326-9-36\n10.1177/0300985814549212\n10.1017/S0007114514003778\n10.2460/ajvr.2004.65.916\n10.2460/javma.2003.223.1576\n10.1139/apnm-2014-0452\n10.1038/nbt.2676\n10.1111/jvim.15629\n10.1038/s41598-019-50563-9\n10.3402/mehd.v26.27663\n10.3920/CEP170003\n10.1111/j.2042-3306.2002.tb05418.x\n10.1111/evj.12132\n10.1186/s12970-020-00353-w\n10.3390/jcm8040452\n10.1016/j.intimp.2008.05.015\n10.2460/ajvr.2003.64.1401\n10.1016/0169-2607(86)90106-9\n10.1093/bioinformatics/btu494\n10.1186/s40168-017-0320-4\n10.1080/09291016.2012.692258\n10.1152/japplphysiol.01349.2006\n10.3390/ani14050727\n10.2460/ajvr.76.1.84\n10.1016/j.jevs.2019.02.011\n10.12688/f1000research.20204.1\n10.1126/science.1241214\n10.1021/jf503314a\n10.1111/evj.12479\n10.2337/dc09-0032\n10.1111/evj.12413\n10.1111/j.2042-3306.2006.tb05544.x\n10.1016/j.jevs.2020.102988\n10.3390/metabo13060718\n10.1016/j.mib.2017.11.006\n10.3389/fphys.2023.1256217\n10.1016/j.jevs.2008.12.006\n10.3389/fcimb.2019.00455\n10.3389/fvets.2020.00126\n10.1371/journal.pone.0107460",
    "journal": "Frontiers in physiology",
    "keywords": [
        "exercise",
        "insulin sensitivity",
        "intravenous and per oral glucose tolerance test",
        "metabolism",
        "microbiome",
        "nutrition"
    ],
    "methods": null,
    "publication_date": "2025-04-25",
    "pubmed_id": "40276369\n26153514\n31133304\n17719811\n30294603\n31341288\n35954878\n10502080\n33912605\n14709204\n36986264\n22452734\n20383131\n20187833\n31586394\n32782301\n25021423\n29233686\n26626721\n25644524\n22626511\n35123855\n37661739\n21816118\n26530154\n35392373\n33848308\n16820507\n12610001\n30724412\n2920697\n27502158\n24670791\n35247098\n32098884\n30531976\n12669265\n22876171\n33498857\n6641685\n3516677\n25217888\n25232034\n25585164\n15281649\n14664443\n25962839\n23975157\n31617618\n31594971\n26028277\n12405686\n23819851\n32398103\n30987356\n18687304\n14620777\n3640682\n25061070\n28797298\n17585040\n38473112\n25535665\n31133313\n32047611\n24009397\n25238637\n26122549\n19592624\n25582152\n17402423\n32534767\n37367876\n29166621\n37654675\n32010641\n32373631\n25202990",
    "results": "Training alone: Training significantly improved OGTT parameters by decreasing Maximum",
    "title": "Aleurone supplementation enhances the metabolic benefits of training in Standardbred mares: impacts on glucose-insulin dynamics and gut microbiome composition.",
    "xml": "<Element 'PubmedArticle' at 0x788505403e70>"
}