{
    "abstract": "Neuropeptide Y (NPY) has been recently characterized in the goldfish and the sequence deduced from goldfish brain cDNA clones reveals that goldfish NPY (gNPY) has over 86% identity with human NPY (hNPY) (Blomqvist et al., 1992, Proc. Natl. Acad. Sci. USA 89, 2350-2354). In the present study, we used synthetic gNPY to investigate the role of NPY in the regulation of growth hormone (GH) and gonadotropin-II (GtH-II) in the goldfish. The dose-response relationships of gNPY on GH and GtH-II release were first tested using an in vitro perifusion system for pituitary fragments. It was found that gNPY stimulated both GH and GtH-II release in a dose-dependent manner, with ED50 values of 1.89 +/- 0.9 nM for GH and 4.19 +/- 2.9 nM for GtH-II. In addition, the C-terminal fragment gNPY (18-36) stimulated the release of both GH and GtH-II, but with lower potency than did the intact molecule. These results confirm our previous findings using hNPY. The interactions of gNPY and other GH and GtH-II regulators were also examined in the present studies. Prolonged infusion of sGnRH induced initial peak releases of GH and GtH-II, followed by a second phase of sustained hormone release at a reduced level. Application of a 5-min pulse of gNPY during the second phase of sGnRH action further stimulated GH and GtH-II release, but only to the levels similar to those induced by gNPY alone. Prolonged infusion with gNPY induced a rapid desensitization of GH and GtH-II release; the hormone levels returned to basal within 25 min in the continued presence of gNPY. Administration of 5-min pulse of sGnRH during the desensitization phase of continuous gNPY perfusion induced a similar GH response to that induced by sGnRH alone, whereas the GtH-II responses to sGnRH given during gNPY infusion were smaller than the responses to sGnRH alone, suggesting that the mechanisms of gNPY stimulation on GH and GtH-II release may be somewhat different. The gNPY-induced GH release was blocked by somatostatin, a known GH inhibitor; the gNPY-induced GtH-II release was reduced by dopamine, a known inhibitor for GtH-II. Finally, intraperitoneal injection of gNPY induced time- and dose-dependent increases in serum GH and GtH-II levels. Together, these results suggest that NPY may play a physiological role in the regulation of GH and GtH-II secretion in the goldfish.",
    "authors": [
        {
            "affiliation": "Department of Zoology, University of Alberta, Edmonton, Canada.",
            "firstname": "C",
            "initials": "C",
            "lastname": "Peng"
        },
        {
            "affiliation": null,
            "firstname": "S",
            "initials": "S",
            "lastname": "Humphries"
        },
        {
            "affiliation": null,
            "firstname": "R E",
            "initials": "RE",
            "lastname": "Peter"
        },
        {
            "affiliation": null,
            "firstname": "J E",
            "initials": "JE",
            "lastname": "Rivier"
        },
        {
            "affiliation": null,
            "firstname": "A G",
            "initials": "AG",
            "lastname": "Blomqvist"
        },
        {
            "affiliation": null,
            "firstname": "D",
            "initials": "D",
            "lastname": "Larhammar"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.1006/gcen.1993.1086",
    "journal": "General and comparative endocrinology",
    "keywords": [],
    "methods": null,
    "publication_date": "1993-06-01",
    "pubmed_id": "7901118",
    "results": null,
    "title": "Actions of goldfish neuropeptide Y on the secretion of growth hormone and gonadotropin-II in female goldfish.",
    "xml": "<Element 'PubmedArticle' at 0x788502c537e0>"
}