{
    "abstract": "N -acetyl-cysteine (NAC) is a potent antioxidant that can be used for many gynecological diseases such as polycystic ovary syndrome and endometriosis. Controlled ovarian hyperstimulation (COH) is a critical step in infertility treatment. Our previous clinical studies have shown that repeated COH led to oxidative stress in follicle fluid and ovarian granulosa cells.\nIn this study, we investigated whether NAC could inhibit oxidative stress in mice caused by repeated COH and improve the mitochondrial function of oocytes.\nFemale Institute of Cancer Research (ICR) mice were randomly assigned into three groups: normal group, model (repeated COH) group, NAC group. We examined the morphology, number and quality of mitochondria. The mechanism of regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) by NAC to ameliorate oxidative stress was also investigated.\nRepeated COH caused oxidative damage in ovaries and oocytes and decreased oocyte quality, while NAC prevented oxidative damage and increased oocyte mitochondrial function. In in vitro experiments, it was verified that NAC can promote the nuclear translocation of Nrf2, which transcriptionally activates the expression of superoxide dismutase and glutathione peroxidase, which removed excessive reactive oxygen species that causes mitochondria damage.\nThe results suggest that NAC raises mitochondrial function in oocytes and improves oocyte quality through decreasing oxidative stress in mice with repeated COH. The underlying mechanism is related to the regulation of the Nrf2 signaling pathway.\nThis study provides a meaningful foundation for the future clinical application of NAC during repeated COH.",
    "authors": [
        {
            "affiliation": "College of Integrative Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, China; and Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Lijie",
            "initials": "L",
            "lastname": "Fan"
        },
        {
            "affiliation": "Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Fengli",
            "initials": "F",
            "lastname": "Guan"
        },
        {
            "affiliation": "Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Yucong",
            "initials": "Y",
            "lastname": "Ma"
        },
        {
            "affiliation": "Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Yu",
            "initials": "Y",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Reproductive Medicine Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.",
            "firstname": "Li",
            "initials": "L",
            "lastname": "Li"
        },
        {
            "affiliation": "Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Ying",
            "initials": "Y",
            "lastname": "Sun"
        },
        {
            "affiliation": "Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Can",
            "initials": "C",
            "lastname": "Cao"
        },
        {
            "affiliation": "College of Integrative Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, China; and Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Huilan",
            "initials": "H",
            "lastname": "Du"
        },
        {
            "affiliation": "Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050091, China.",
            "firstname": "Ming",
            "initials": "M",
            "lastname": "He"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.1071/RD22020",
    "journal": "Reproduction, fertility, and development",
    "keywords": [],
    "methods": null,
    "publication_date": "2022-05-06",
    "pubmed_id": "35513370",
    "results": null,
    "title": "",
    "xml": "<Element 'PubmedArticle' at 0x788504ebcd10>"
}