{
    "abstract": "The role of sulfur-containing drugs, disulfiram (DSF) and N-acetylcysteine (NAC), in alleviating neuroinflammation is poorly understood. The objective of this study was to examine the effect of DSF and NAC on memory and on the metabolism of L-cysteine and inflammation-related parameters in the cerebral cortex of rats in a model of neuroinflammation induced by the administration of lipopolysaccharide (LPS).\nAll the treatments were administered intraperitoneally for 10 days (LPS at a dose of 0.5 mg/kg b.w., DSF at a dose of 100 mg/kg b.w, and NAC at a dose of 100 mg/kg b.w.). Behavior was evaluated by the novel object recognition (NOR) test and object location (OL) test, and the level of brain-derived neurotrophic factor (BDNF) was assayed to evaluate neuronal functioning. Cerebral cortex homogenates were tested for hydrogen sulfide (H\nNeither DSF nor NAC alleviated LPS-induced memory disorders estimated by the NOR test and OL test. The studied compounds also did not affect significantly the levels of BDNF, ROS, NO, H\nOur study did not confirm the suggested potential of DSF and NAC to correct memory disorders; however, it corroborated the notion that they reduced oxidative stress induced by LPS by increasing the NPSH level. Additionally, our study showed an increase in sulfate concentration in the brain tissues after LPS treatment, which means the upregulation of sulfite and sulfate production in inflammatory conditions.",
    "authors": [
        {
            "affiliation": "Chair of Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7, PL 31-034 Cracow, Poland.",
            "firstname": "Ma\u0142gorzata",
            "initials": "M",
            "lastname": "Iciek"
        },
        {
            "affiliation": "Chair of Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7, PL 31-034 Cracow, Poland.",
            "firstname": "Anna",
            "initials": "A",
            "lastname": "Bilska-Wilkosz"
        },
        {
            "affiliation": "Chair of Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7, PL 31-034 Cracow, Poland.",
            "firstname": "Magdalena",
            "initials": "M",
            "lastname": "G\u00f3rny"
        },
        {
            "affiliation": "Department of Pharmacological Screening, Jagiellonian University Medical College, Medyczna 9, PL 30-688 Cracow, Poland.",
            "firstname": "Marek",
            "initials": "M",
            "lastname": "Bednarski"
        },
        {
            "affiliation": "Department of Pharmacological Screening, Jagiellonian University Medical College, Medyczna 9, PL 30-688 Cracow, Poland.",
            "firstname": "Ma\u0142gorzata",
            "initials": "M",
            "lastname": "Zygmunt"
        },
        {
            "affiliation": "Department of Pharmacological Screening, Jagiellonian University Medical College, Medyczna 9, PL 30-688 Cracow, Poland.\nDepartment of Veterinary Sciences, University of Messina, 98168 Messina, Italy.",
            "firstname": "Anthea",
            "initials": "A",
            "lastname": "Miller"
        },
        {
            "affiliation": "PhD Program in Neuroscience, School of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy.\nSchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy.",
            "firstname": "Noemi",
            "initials": "N",
            "lastname": "Nicosia"
        },
        {
            "affiliation": "Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres 31, 98166 Messina, Italy.",
            "firstname": "Giorgia Pia",
            "initials": "GP",
            "lastname": "Lombardo"
        },
        {
            "affiliation": "Department of Pharmacological Screening, Jagiellonian University Medical College, Medyczna 9, PL 30-688 Cracow, Poland.\nDepartment of Pharmacy, Faculty of Medicine and Surgery, University of Malta, MSD 2080 Msida, Malta.",
            "firstname": "Paula",
            "initials": "P",
            "lastname": "Zammit"
        },
        {
            "affiliation": "Department of Pharmacological Screening, Jagiellonian University Medical College, Medyczna 9, PL 30-688 Cracow, Poland.",
            "firstname": "Magdalena",
            "initials": "M",
            "lastname": "Kota\u0144ska"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.3390/molecules30030578\n10.1186/s12974-015-0434-1\n10.1016/j.neulet.2018.09.010\n10.1007/s00210-016-1234-6\n10.1016/j.jnutbio.2018.01.009\n10.33549/physiolres.933480\n10.1523/JNEUROSCI.16-03-01066.1996\n10.1016/j.nlm.2013.05.006\n10.1096/fj.12-226415\n10.1007/s12640-021-00374-6\n10.1016/j.freeradbiomed.2017.10.346\n10.1089/ars.2020.8073\n10.1016/j.freeradbiomed.2009.09.018\n10.1042/BSR20221006\n10.1371/journal.pone.0078332\n10.1016/j.cbi.2010.04.011\n10.1007/s00213-017-4762-y\n10.1203/01.pdr.0000215045.91122.44\n10.1016/j.neubiorev.2017.04.013\n10.1016/j.pharmthera.2021.107916\n10.1111/j.1600-0447.1992.tb03309.x\n10.2174/1574892814666190514104035\n10.3389/fcimb.2021.633194\n10.1007/s11739-021-02633-y\n10.3390/antiox11061053\n10.1016/S2352-3018(15)00226-X\n10.1073/pnas.2306399120\n10.1038/s41598-018-19577-7\n10.1016/j.bcp.2020.114267\n10.1016/j.jneuroim.2020.577401\n10.1038/s41598-019-42286-8\n10.3791/58593-v\n10.4103/ijpvm.IJPVM_170_19\n10.1080/1028415X.2022.2063578\n10.1007/s11011-018-0349-5\n10.1007/s11064-016-1881-5\n10.1021/acschemneuro.5b00274\n10.1016/j.pharep.2014.10.014\n10.1016/j.bbi.2007.08.014\n10.1055/s-0042-103592\n10.1111/j.1530-0277.1980.tb04841.x\n10.1016/0028-3908(83)90055-2\n10.1111/j.1600-0447.1992.tb03310.x\n10.18632/oncotarget.23320\n10.1016/j.cbi.2016.04.026\n10.3389/fnagi.2018.00399\n10.1016/j.bbagen.2013.04.016\n10.1016/S1054-3589(08)61042-X\n10.1111/j.1471-4159.1982.tb12548.x\n10.1007/s13311-023-01422-z\n10.1021/acschemneuro.3c00180\n10.1016/j.redox.2020.101576\n10.1023/A:1021191729865\n10.3390/ijms24043798\n10.1007/s11064-024-04105-z\n10.1016/j.eatbeh.2014.10.008\n10.1007/s10637-022-01271-1\n10.1016/j.nlm.2007.08.018\n10.5604/20831862.1163692\n10.1371/journal.pone.0033527\n10.1038/tp.2015.183\n10.1016/j.npep.2015.09.003\n10.1016/j.crphar.2022.100091\n10.1016/j.ejmech.2022.115071\n10.1152/physrev.00029.2006\n10.1038/jcbfm.2011.48\n10.1007/s11481-020-09920-z\n10.1016/j.ejphar.2023.175743\n10.1016/j.neures.2023.07.004\n10.1016/S0006-2952(00)00435-4\n10.2174/156800911794519798\n10.1016/j.freeradbiomed.2015.10.410\n10.1039/D1SC03828A\n10.1097/01.shk.0000183393.83272.de\n10.3390/biomedicines9091252\n10.1165/ajrcmb.17.2.2764\n10.1155/2019/1974982\n10.1016/0006-8993(91)90915-I\n10.1016/j.pnpbp.2012.06.018\n10.1016/j.phrs.2003.09.013\n10.1371/journal.pone.0189821\n10.1371/journal.pone.0141327\n10.1016/0926-6917(94)90011-6\n10.1016/j.freeradbiomed.2011.01.025\n10.1016/0003-2697(68)90092-4\n10.1016/S0021-9258(19)52451-6",
    "journal": "Molecules (Basel, Switzerland)",
    "keywords": [
        "N-acetylcysteine",
        "cognitive disorders",
        "disulfiram",
        "hydrogen sulfide",
        "lipopolysaccharide",
        "neuroinflammation",
        "non-protein thiols",
        "novel object recognition",
        "sulfane sulfur",
        "sulfate"
    ],
    "methods": null,
    "publication_date": "2025-02-13",
    "pubmed_id": "39942681\n26608623\n30213621\n27026404\n29454265\n28406691\n8558235\n23726868\n23413359\n34021860\n29055825\n33198504\n19770036\n36039860\n24205200\n20399762\n29058042\n16627882\n28438466\n34171332\n1471556\n31084595\n33777846\n33464469\n35739949\n26614966\n37487070\n29358714\n33035509\n33002724\n30962497\n30531711\n33209221\n35470773\n30456649\n26961891\n26689453\n25712666\n17951027\n26987743\n7004242\n6633826\n1471547\n29423059\n27087133\n30574085\n23618697\n8068563\n7276953\n37596428\n37216423\n32502899\n10591399\n36835209\n38381245\n25464072\n35763178\n17942328\n26681831\n22442695\n26670281\n26396035\n27335702\n35243333\n36603509\n17237348\n21487413\n32676889\n37084816\n37442198\n10974203\n21247383\n26476010\n34745524\n16317383\n34572437\n9271311\n31885774\n1655165\n22820675\n14726217\n29261743\n26506439\n7805784\n21276849\n3657542\n4973948\n14907713",
    "results": "Neither DSF nor NAC alleviated LPS-induced memory disorders estimated by the NOR test and OL test. The studied compounds also did not affect significantly the levels of BDNF, ROS, NO, H",
    "title": "The Effect of Disulfiram and N-Acetylcysteine, Potential Compensators for Sulfur Disorders, on Lipopolysaccharide-Induced Neuroinflammation Leading to Memory Impairment and the Metabolism of L-Cysteine Disturbance.",
    "xml": "<Element 'PubmedArticle' at 0x788503a927a0>"
}