{
    "abstract": "Red blood cells (RBCs) are suggested to play a role in cardiovascular regulation by exporting nitric oxide (NO) bioactivity and ATP under hypoxia. It remains unknown whether such beneficial effects of RBCs are protective in patients with acute myocardial infarction. We investigated whether RBCs from patients with ST-elevation myocardial infarction (STEMI) protect against myocardial ischemia-reperfusion injury and whether such effect involves NO and purinergic signaling in the RBCs. RBCs from patients with STEMI undergoing primary coronary intervention and healthy controls were administered to isolated rat hearts subjected to global ischemia and reperfusion. Compared to RBCs from healthy controls, RBCs from STEMI patients reduced myocardial infarct size (30\u2009\u00b1\u200912% RBC healthy vs. 11\u2009\u00b1\u20095% RBC STEMI patients, P\u2009<\u20090.001), improved recovery of left-ventricular developed pressure and dP/dt and reduced left-ventricular end-diastolic pressure in hearts subjected to ischemia-reperfusion. Inhibition of RBC NO synthase with L-NAME or soluble guanylyl cyclase (sGC) with ODQ, and inhibition of cardiac protein kinase G (PKG) abolished the cardioprotective effect. Furthermore, the non-selective purinergic P2 receptor antagonist PPADS but not the P1 receptor antagonist 8PT attenuated the cardioprotection induced by RBCs from STEMI patients. The P2Y",
    "authors": [
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden. tong.jiao@ki.se.",
            "firstname": "Tong",
            "initials": "T",
            "lastname": "Jiao"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.",
            "firstname": "Aida",
            "initials": "A",
            "lastname": "Collado"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.",
            "firstname": "Ali",
            "initials": "A",
            "lastname": "Mahdi"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.\nDepartment of Cardiology, Karolinska University Hospital, Stockholm, Sweden.",
            "firstname": "Juliane",
            "initials": "J",
            "lastname": "Jurga"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.",
            "firstname": "John",
            "initials": "J",
            "lastname": "Tengbom"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.\nDepartment of Cardiology, Karolinska University Hospital, Stockholm, Sweden.",
            "firstname": "Nawzad",
            "initials": "N",
            "lastname": "Saleh"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.\nDepartment of Cardiology, Karolinska University Hospital, Stockholm, Sweden.",
            "firstname": "Dinos",
            "initials": "D",
            "lastname": "Verouhis"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.\nDepartment of Cardiology, Karolinska University Hospital, Stockholm, Sweden.",
            "firstname": "Felix",
            "initials": "F",
            "lastname": "B\u00f6hm"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.",
            "firstname": "Zhichao",
            "initials": "Z",
            "lastname": "Zhou"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden.",
            "firstname": "Jiangning",
            "initials": "J",
            "lastname": "Yang"
        },
        {
            "affiliation": "Department of Medicine, Division of Cardiology, Karolinska Institutet, Stockholm, Sweden. john.pernow@ki.se.\nDepartment of Cardiology, Karolinska University Hospital, Stockholm, Sweden. john.pernow@ki.se.",
            "firstname": "John",
            "initials": "J",
            "lastname": "Pernow"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2022. The Author(s).",
    "doi": "10.1007/s00395-022-00953-4\n10.1016/s0735-1097(00)00804-4\n10.1038/s41598-019-48006-6\n10.1023/a:1012388618693\n10.1007/s00395-018-0696-8\n10.1007/s11302-015-9462-7\n10.1007/s11302-014-9436-1\n10.1016/j.redox.2013.12.027\n10.1016/j.redox.2017.08.020\n10.1016/j.redox.2022.102370\n10.1038/nm954\n10.1182/blood-2005-07-2668\n10.1161/CIRCULATIONAHA.108.764571\n10.1016/j.jacc.2018.09.086\n10.1111/j.1742-4658.2006.05591.x\n10.1007/s11010-016-2723-0\n10.1002/cphy.c090009\n10.1111/apha.12596\n10.1016/j.cardiores.2006.06.011\n10.1038/s41569-020-0403-y\n10.1161/CIRCULATIONAHA.108.805242\n10.1007/s11302-013-9374-3\n10.1038/380221a0\n10.1182/blood-2005-10-3992\n10.1007/s00395-021-00893-5\n10.1161/CIRCULATIONAHA.120.049606\n10.1016/j.cmet.2018.06.007\n10.1016/j.freeradbiomed.2021.03.020\n10.3389/fphar.2019.00861\n10.3389/fphar.2020.603226\n10.1016/j.cardiores.2006.12.020\n10.1016/j.molbiopara.2019.02.007\n10.1007/s00395-013-0398-1\n10.1016/j.niox.2020.01.008\n10.1038/35054560\n10.1016/0014-2999(87)90712-6\n10.3389/fphys.2018.00690\n10.3389/fmolb.2015.00060\n10.1111/j.1549-8719.2011.00158.x\n10.1161/CIRCULATIONAHA.118.039284\n10.3233/CH-170326\n10.1161/CIRCULATIONAHA.116.024014\n10.1161/01.RES.0000153670.07559.E4\n10.1007/s00395-020-0799-x\n10.1073/pnas.1307058110\n10.1016/j.jacbts.2018.03.006\n10.1056/NEJMra071667\n10.1007/s11302-021-09807-5\n10.2337/db21-0093\n10.1016/j.jacc.2018.05.052",
    "journal": "Basic research in cardiology",
    "keywords": [
        "ATP",
        "Ischemia\u2013reperfusion injury",
        "Nitric oxide",
        "Purinergic",
        "Red blood cells",
        "ST-elevation myocardial infarction",
        "Soluble guanylyl cyclase"
    ],
    "methods": null,
    "publication_date": "2022-09-17",
    "pubmed_id": "36112326\n10987628\n31406184\n11699948\n30120595\n26260710\n25527177\n24494200\n29024896\n35759945\n14595407\n16195332\n19188511\n30621955\n17229149\n27206740\n23737185\n26336065\n16836989\n32620851\n18981312\n23828651\n8637569\n16368881\n34515837\n34229449\n29972800\n33789125\n31427970\n33390992\n17258181\n30831155\n24346018\n31981741\n11214321\n3595720\n29930515\n26579528\n22775760\n30905171\n29036803\n27789556\n15604418\n32533377\n23980179\n30175269\n17855673\n34410591\n34753800\n30092954",
    "results": null,
    "title": "Erythrocytes from patients with ST-elevation myocardial infarction induce cardioprotection through the purinergic P2Y",
    "xml": "<Element 'PubmedArticle' at 0x788502fbfb00>"
}