{
    "abstract": "This study aimed to investigate the clinical infection characteristics and analyze the resistance gene carrying status of carbapenem-resistant Providencia rettgeri via whole genome sequencing (WGS).\nCarbapenem-resistant P. rettgeri were collected from clinical patients between January 2020 and December 2021, and their susceptibility to 19 antimicrobial drugs was determined using the VITEK 2 Compact system and Kirby-Bauer (KB) disk diffusion method. The Illumina platform was used to perform WGS of the P. rettgeri isolates, and the resistance genes carried by the Carbapenem-resistant P. rettgeri strains were detected via ABRicate software. The phylogenetic tree was constructed by thirty-four strains including twenty-eight strains downloaded from NCBI database and the carbapenem-resistant six P. rettgeri strains in this study. Which based on genomic single nucleotide polymorphism (SNP) to understand the affinities of the carbapenem-resistant P. rettgeri strains.\nSix carbapenem-resistant P. rettgeri strains were isolated from five different clinical departments using the blood, urine, sputum, and secretion specimens. These infected patients are middle-aged and elderly people with a history of severe trauma, tumors, hypertension, and various other underlying diseases, and invasive procedures. Antimicrobial sensitivity testing showed that all strains presented resistance to ampicillin-sulbactam, ceftazidime, ciprofloxacin, levofloxacin, and ertapenem, whereas they exhibited full susceptibility to cefepime and amikacin. Most strains demonstrated high resistance to \u03b2-lactams, aminoglycosides, and sulfonamides. Thirty-five resistance genes were identified by ABRicate. All carbapenem-resistant P. rettgeri strains carried aminoglycoside, fluoroquinolone, chloramphenicol, rifampicin, sulfonamide, and \u03b2-lactam resistance genes, and most importantly, all strains possessed the carbapenem resistance gene bla\nCarbapenem-resistant P. rettgeri strains were mostly isolated from middle-aged and older patients with a history of surgery or serious underlying diseases, and they were found to cause multisystem infections. All Carbapenem-resistant P. rettgeri strains in this study carried bla",
    "authors": [
        {
            "affiliation": "Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong, China.",
            "firstname": "Mi",
            "initials": "M",
            "lastname": "Liu"
        },
        {
            "affiliation": "Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong, China.",
            "firstname": "Na",
            "initials": "N",
            "lastname": "Yi"
        },
        {
            "affiliation": "Clinical Medicine, Shandong First Medical University, Taian, Shandong, China.",
            "firstname": "Xinyi",
            "initials": "X",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong, China. 20897318@qq.com.",
            "firstname": "Rongrong",
            "initials": "R",
            "lastname": "Wang"
        }
    ],
    "conclusions": "Carbapenem-resistant P. rettgeri strains were mostly isolated from middle-aged and older patients with a history of surgery or serious underlying diseases, and they were found to cause multisystem infections. All Carbapenem-resistant P. rettgeri strains in this study carried bla",
    "copyrights": "\u00a9 2023. BioMed Central Ltd., part of Springer Nature.",
    "doi": "10.1186/s12866-023-03032-3\n10.1099/00222615-47-7-629\n10.1128/AAC.00382-20\n10.1016/j.nmni.2018.05.009\n10.1016/S1473-3099(10)70143-2\n10.1111/nyas.14237\n10.1089/cmb.2012.0021\n10.1128/AAC.00483-19\n10.1128/AAC.02412-14\n10.1093/molbev/msy096\n10.1093/nar/gkz239\n10.1016/j.jgar.2019.07.013\n10.1186/s12941-018-0272-y\n10.3389/fcimb.2021.789646\n10.3171/CASE21318\n10.1007/s10096-014-2097-8\n10.1186/s13756-019-0608-1\n10.1186/s12866-020-02030-z\n10.1186/s13756-019-0491-9\n10.3390/microorganisms8071074\n10.1128/AAC.00071-13\n10.3389/fmicb.2020.572642",
    "journal": "BMC microbiology",
    "keywords": [
        "Carbapenem-resistant Providencia rettgeri",
        "Whole genome sequencing"
    ],
    "methods": null,
    "publication_date": "2023-10-04",
    "pubmed_id": "37789331\n9839568\n32816727\n29983987\n20705517\n31549428\n22506599\n31427293\n24777092\n29722887\n30931475\n31325615\n29728111\n35047418\n35855091\n33403565\n24798249\n31636898\n33183231\n29496594\n30792853\n32708454\n23571552\n33193173",
    "results": "Six carbapenem-resistant P. rettgeri strains were isolated from five different clinical departments using the blood, urine, sputum, and secretion specimens. These infected patients are middle-aged and elderly people with a history of severe trauma, tumors, hypertension, and various other underlying diseases, and invasive procedures. Antimicrobial sensitivity testing showed that all strains presented resistance to ampicillin-sulbactam, ceftazidime, ciprofloxacin, levofloxacin, and ertapenem, whereas they exhibited full susceptibility to cefepime and amikacin. Most strains demonstrated high resistance to \u03b2-lactams, aminoglycosides, and sulfonamides. Thirty-five resistance genes were identified by ABRicate. All carbapenem-resistant P. rettgeri strains carried aminoglycoside, fluoroquinolone, chloramphenicol, rifampicin, sulfonamide, and \u03b2-lactam resistance genes, and most importantly, all strains possessed the carbapenem resistance gene bla",
    "title": "Analysis of resistance genes of carbapenem-resistant Providencia rettgeri using whole genome sequencing.",
    "xml": "<Element 'PubmedArticle' at 0x788503b61210>"
}