{
    "abstract": "To observe the effect of electroacupuncture (EA) at \"Neiguan\" (PC 6) on myocardial fibrosis in spontaneously hypertensive rats (SHR), and explore preliminarily the mediating role of cholinergic anti-inflammatory pathway (CAP) and its downstream nuclear factor \u03baB (NF-\u03baB) signaling pathway.\nSix 12-week-old WKY male rats were employed as the normal group. Eighteen 12-week-old SHR were randomly divided into 3 groups, i.e. a model group, an EA group and a blocking group (EA after blocking \u03b17 nicotinic acetylcholine receptor [\u03b17nAchR]), with 6 rats in each one. In the EA group, EA was delivered at \"Neiguan\"(PC 6) and the site 0.5 cm from its left side, with disperse-dense wave, 2 Hz/15 Hz in frequency and 1 mA in current intensity. One intervention took 30 min and was given once every 2 days, lasting 8 weeks. In the blocking group, prior to each EA, the \u03b17nAchR specific blocker, \u03b1-bungartoxin was injected intravenously in the tails of the rats. After EA intervention, the systolic blood pressure (SBP), the diastolic blood pressure (DBP) and the mean arterial pressure (MAP) were measured with non-invasive blood pressure monitor. Using echocardiogram, the left ventricular (LV) anterior wall end-diastolic thickness (LVAWd) , LV posterior wall end-diastolic thickness (LVPWd) and the LV end-diastolic internal diameter (LVIDd) were measured. The level of hydroxyproline (Hyp) in the myocardial tissue was determined by using alkaline hydrolysis, and that of acetylcholine (Ach) was detected by ELISA. With the real-time PCR adopted, the mRNA expression of NF-\u03baB p65, tumor necrosis factor \u03b1 (TNF-\u03b1), interleukin (IL)-1\u03b2 and IL-6 were determined.\nCompared with the normal group, SBP, DBP, MAP, LVAWd and LVPWd were increased (\nCAP may be involved in ameliorating the pathological damage of myocardial\u2002fibrosis\u2002during EA at \"Neiguan\"(PC 6). The underlying effect mechanism is associated with up-regulating the neurotransmitter, Ach and down-regulating mRNA expression of NF-\u03baB p65 and pro-inflammatory factors such as TNF-\u03b1, IL-1\u03b2 and IL-6 in myocardial tissue.",
    "authors": [
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Juan-Juan",
            "initials": "JJ",
            "lastname": "Xin"
        },
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Chen",
            "initials": "C",
            "lastname": "Zhou"
        },
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Shuang",
            "initials": "S",
            "lastname": "Wu"
        },
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Wen-Xi",
            "initials": "WX",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Qun",
            "initials": "Q",
            "lastname": "Liu"
        },
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Yu-Xue",
            "initials": "YX",
            "lastname": "Zhao"
        },
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Xiao-Chun",
            "initials": "XC",
            "lastname": "Yu"
        },
        {
            "affiliation": "Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.",
            "firstname": "Jun-Hong",
            "initials": "JH",
            "lastname": "Gao"
        }
    ],
    "conclusions": "CAP may be involved in ameliorating the pathological damage of myocardial\u2002fibrosis\u2002during EA at \"Neiguan\"(PC 6). The underlying effect mechanism is associated with up-regulating the neurotransmitter, Ach and down-regulating mRNA expression of NF-\u03baB p65 and pro-inflammatory factors such as TNF-\u03b1, IL-1\u03b2 and IL-6 in myocardial tissue.",
    "copyrights": null,
    "doi": "10.13703/j.0255-2930.20221111-k0004",
    "journal": "Zhongguo zhen jiu = Chinese acupuncture & moxibustion",
    "keywords": [
        "Point PC 6 (Neiguan)",
        "cholinergic anti-inflammatory pathway",
        "electroacupuncture",
        "inflammatory cytokine",
        "myocardial fibrosis",
        "spontaneous hypertension"
    ],
    "methods": null,
    "publication_date": "2023-10-07",
    "pubmed_id": "37802521",
    "results": "Compared with the normal group, SBP, DBP, MAP, LVAWd and LVPWd were increased (",
    "title": "[Effect of electroacupuncture on myocardial fibrosis in spontaneously hypertensive rats based on cholinergic anti-inflammatory pathway].",
    "xml": "<Element 'PubmedArticle' at 0x7885057053a0>"
}