{
    "abstract": "Neutrophil extracellular traps (NETs) are web-like complexes of DNA and proteins that are extruded by activated neutrophils and play critical roles as major components of the innate immune response against pathogen invasion. However, some microbes have developed strategies to evade NET attacks, leading to impaired immune defenses and persistent infections. In this study, an engineered neutrophil strategy for enhancing the antibacterial activity of NETs is developed. A nucleus-targeting photosensitizer (NCP) with strong reactive oxygen species production and a strong DNA-binding capacity is synthesized. NCP-loaded neutrophils are subsequently constructed via direct incubation of NCP with neutrophils, and the NCP is closely inserted into the nucleus DNA. Upon activation by bacteria-related toxins, NCP-coupled NETs can be released rapidly, actively trapping bacteria and providing a high local concentration of NCP around them. Both in vitro and in vivo results revealed that NCP-coupled NETs can effectively eradicate various multidrug-resistant bacteria and biofilms through photodynamic therapy, overcome bacterial immune evasion, and promote tissue recovery from severe wound infections. This design can significantly strengthen NET function, providing a non-antibiotic alternative platform for treating bacterial infectious diseases.",
    "authors": [
        {
            "affiliation": "Department of Burns, Wound Repair & Reconstruction, Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Sun Yat-Sen University, Guangzhou, 510080, China.\nGuangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, 510515, China.",
            "firstname": "Peng",
            "initials": "P",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Burns, Wound Repair & Reconstruction, Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Sun Yat-Sen University, Guangzhou, 510080, China.",
            "firstname": "Qingqing",
            "initials": "Q",
            "lastname": "Bai"
        },
        {
            "affiliation": "Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117585, Singapore.",
            "firstname": "Xianglong",
            "initials": "X",
            "lastname": "Liu"
        },
        {
            "affiliation": "Department of Burns, Wound Repair & Reconstruction, Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Sun Yat-Sen University, Guangzhou, 510080, China.",
            "firstname": "Minyang",
            "initials": "M",
            "lastname": "Zhao"
        },
        {
            "affiliation": "Department of Burns, Wound Repair & Reconstruction, Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Sun Yat-Sen University, Guangzhou, 510080, China.",
            "firstname": "Lei",
            "initials": "L",
            "lastname": "Chen"
        },
        {
            "affiliation": "Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, 510515, China.",
            "firstname": "Fang",
            "initials": "F",
            "lastname": "Hu"
        },
        {
            "affiliation": "Institute of Infectious Diseases, Shenzhen Bay Laboratory, Shenzhen, 518132, China.",
            "firstname": "Jinzhou",
            "initials": "J",
            "lastname": "Ye"
        },
        {
            "affiliation": "Institute of Infectious Diseases, Shenzhen Bay Laboratory, Shenzhen, 518132, China.",
            "firstname": "Xinhan",
            "initials": "X",
            "lastname": "Chen"
        },
        {
            "affiliation": "State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China.",
            "firstname": "Kang-Nan",
            "initials": "KN",
            "lastname": "Wang"
        },
        {
            "affiliation": "Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117585, Singapore.",
            "firstname": "Bin",
            "initials": "B",
            "lastname": "Liu"
        },
        {
            "affiliation": "Department of Burns, Wound Repair & Reconstruction, Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Sun Yat-Sen University, Guangzhou, 510080, China.",
            "firstname": "Duo",
            "initials": "D",
            "lastname": "Mao"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2024 Wiley\u2010VCH GmbH.",
    "doi": "10.1002/adma.202400304",
    "journal": "Advanced materials (Deerfield Beach, Fla.)",
    "keywords": [
        "antibacterial photodynamic therapy",
        "multidrug\u2010resistant bacterial infection",
        "neutrophil extracellular traps",
        "nucleus\u2010targeting photosensitizer"
    ],
    "methods": null,
    "publication_date": "2024-11-13",
    "pubmed_id": "39529561",
    "results": null,
    "title": "Nucleus-Targeting Photosensitizers Enhance Neutrophil Extracellular Traps for Efficient Eradication of Multidrug-Resistant Bacterial Infections.",
    "xml": "<Element 'PubmedArticle' at 0x7885039167f0>"
}