{
    "abstract": "To investigate alterations of the global DNA methylation profile in placenta, cord blood, and neonatal buccal smears in infants conceived using in\u00a0vitro maturation (IVM) with a prematuration step (capacitation-IVM [CAPA-IVM]) vs. in\u00a0vitro fertilization (IVF).\nAnalysis of data from the offspring of participants in a randomized controlled trial.\nPrivate clinic.\nForty-six women with polycystic ovary syndrome and/or high antral follicle count and their offspring (58 newborns).\nWomen with polycystic ovary syndrome and/or a high antral follicle count participating in the clinical trial were randomized to undergo CAPA-IVM or conventional IVF.\nAt delivery, biological samples including cord blood, placental tissue, and a neonatal buccal smear were collected. Genome-wide DNA methylation was determined using the Illumina Infinium MethylationEPIC BeadChip. Variability in methylation was also considered, and mean variances for the two treatment categories were compared.\nIn neonatal buccal smears, there were no significant differences between the CAPA-IVM and conventional IVF groups on the basis of the CpG probe after linear regression analysis using a significant cut-off of false-discovery rate <0.05 and |\u0394\u03b2|\u22650.05. In cord blood, only one CpG site showed a significant gain of methylation in the CAPA-IVM group. In the placenta, CAPA-IVM was significantly associated with changes in methylation at five CpG sites. Significantly more variable DNA methylation was found in five probes in the placenta, 54 in cord blood, and two in buccal smears after IVM of oocytes. In cord blood samples, 20 CpG sites had more variable methylation in the conventional IVF vs. IVM group. Isolated CpG sites showing differences in methylation in cord blood were not associated with changes in gene expression of the overlapping genes.\nCapacitation-IVM appeared to be associated with only a small amount of epigenetic variation in cord blood, placental tissue, and neonate buccal smears.\nNCT03405701 (www.\ngov).",
    "authors": [
        {
            "affiliation": "Follicle Biology Laboratory, UZ Brussel, Vrije Universiteit Brussel, Brussel, Belgium.",
            "firstname": "Laura",
            "initials": "L",
            "lastname": "Saucedo-Cuevas"
        },
        {
            "affiliation": "IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam; HOPE Research Center, My Duc Hospital, Ho Chi Minh City, Vietnam.",
            "firstname": "Mai P Q",
            "initials": "MPQ",
            "lastname": "Ma"
        },
        {
            "affiliation": "IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam; HOPE Research Center, My Duc Hospital, Ho Chi Minh City, Vietnam.",
            "firstname": "Anh H",
            "initials": "AH",
            "lastname": "Le"
        },
        {
            "affiliation": "Follicle Biology Laboratory, UZ Brussel, Vrije Universiteit Brussel, Brussel, Belgium.",
            "firstname": "Nazli",
            "initials": "N",
            "lastname": "Akin"
        },
        {
            "affiliation": "IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam; HOPE Research Center, My Duc Hospital, Ho Chi Minh City, Vietnam.",
            "firstname": "Toan D",
            "initials": "TD",
            "lastname": "Pham"
        },
        {
            "affiliation": "IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam; HOPE Research Center, My Duc Hospital, Ho Chi Minh City, Vietnam.",
            "firstname": "Tuong M",
            "initials": "TM",
            "lastname": "Ho"
        },
        {
            "affiliation": "Human Genotyping Unit-CeGen, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.",
            "firstname": "Guillermo",
            "initials": "G",
            "lastname": "Pita"
        },
        {
            "affiliation": "Human Genotyping Unit-CeGen, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.",
            "firstname": "Anna",
            "initials": "A",
            "lastname": "Gonzalez-Neira"
        },
        {
            "affiliation": "Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Brussels, Belgium; Department of Obstetrics, Gynecology, Perinatology, and Reproductology, Institute of Professional Education, Sechenov University, Moscow, Russia.",
            "firstname": "Michel",
            "initials": "M",
            "lastname": "De Vos"
        },
        {
            "affiliation": "Follicle Biology Laboratory, UZ Brussel, Vrije Universiteit Brussel, Brussel, Belgium.",
            "firstname": "Johan",
            "initials": "J",
            "lastname": "Smitz"
        },
        {
            "affiliation": "Follicle Biology Laboratory, UZ Brussel, Vrije Universiteit Brussel, Brussel, Belgium.",
            "firstname": "Ellen",
            "initials": "E",
            "lastname": "Anckaert"
        },
        {
            "affiliation": "Department of Obstetrics and Gynecology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam. Electronic address: drlan@yahoo.com.vn.",
            "firstname": "Lan N",
            "initials": "LN",
            "lastname": "Vuong"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2023 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.",
    "doi": "10.1016/j.fertnstert.2023.11.040",
    "journal": "Fertility and sterility",
    "keywords": [
        "Capacitation-in\u00a0vitro maturation",
        "DNA methylation",
        "imprinted genes",
        "neonatal tissues",
        "oocyte"
    ],
    "methods": null,
    "publication_date": "2023-12-06",
    "pubmed_id": "38052376",
    "results": "In neonatal buccal smears, there were no significant differences between the CAPA-IVM and conventional IVF groups on the basis of the CpG probe after linear regression analysis using a significant cut-off of false-discovery rate <0.05 and |\u0394\u03b2|\u22650.05. In cord blood, only one CpG site showed a significant gain of methylation in the CAPA-IVM group. In the placenta, CAPA-IVM was significantly associated with changes in methylation at five CpG sites. Significantly more variable DNA methylation was found in five probes in the placenta, 54 in cord blood, and two in buccal smears after IVM of oocytes. In cord blood samples, 20 CpG sites had more variable methylation in the conventional IVF vs. IVM group. Isolated CpG sites showing differences in methylation in cord blood were not associated with changes in gene expression of the overlapping genes.",
    "title": "Epigenetic variation in neonatal tissues in infants conceived using capacitation-in\u00a0vitro maturation vs. in\u00a0vitro fertilization.",
    "xml": "<Element 'PubmedArticle' at 0x788506f6cfe0>"
}