{
    "abstract": "6-Aminocaproic acid (6ACA) is a key building block and an attractive precursor of caprolactam, which is used to synthesize nylon\u20056, one of the most common polymers manufactured nowadays. (Bio)-production of platform chemicals from renewable feedstocks is instrumental to tackle climate change and decrease fossil fuel dependence. Here, the cell-free biosynthesis of 6ACA from 6-hydroxycaproic acid was achieved using a co-immobilized multienzyme system based on horse liver alcohol dehydrogenase, Halomonas elongata transaminase, and Lactobacillus pentosus NADH oxidase for in-situ cofactor recycling, with >90\u2009% molar conversion (m.c.) The integration of a step to synthesize hydroxy-acid from lactone by immobilized Candida antarctica lipase B resulted in >80\u2009% m.c. of \u03f5-caprolactone to 6ACA, >20\u2009% of \u03b4-valerolactone to 5-aminovaleric acid, and 30\u2009% of \u03b3-butyrolactone to \u03b3-aminobutyric acid in one-pot batch reactions. Two serial packed-bed reactors were set up using these biocatalysts and applied to the continuous-flow synthesis of 6ACA from \u03f5-caprolactone, achieving a space-time yield of up to 3.31\u2005g",
    "authors": [
        {
            "affiliation": "School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, United Kingdom.",
            "firstname": "Maria",
            "initials": "M",
            "lastname": "Romero-Fernandez"
        },
        {
            "affiliation": "School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, United Kingdom.",
            "firstname": "Christian M",
            "initials": "CM",
            "lastname": "Heckmann"
        },
        {
            "affiliation": "School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, United Kingdom.\nDepartment of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland.",
            "firstname": "Francesca",
            "initials": "F",
            "lastname": "Paradisi"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2022 The Authors. ChemSusChem published by Wiley-VCH GmbH.",
    "doi": "10.1002/cssc.202200811",
    "journal": "ChemSusChem",
    "keywords": [
        "biocatalysis",
        "continuous flow",
        "enzyme cascade",
        "nylon 6",
        "\u03f5-caprolactone"
    ],
    "methods": null,
    "publication_date": "2022-06-08",
    "pubmed_id": "35671069\n12007491\n31886667\n26511532\n32992793\n30905694\n28579174\n28190099\n28876904\n23807146\n31865258\n34636371\n11157238\n29318245\n29337543\n25443804\n35348296\n34220333\n22887645\n23341101\n26404833\n26583050\n15294826\n16039872\n22544564\n26441891\n28752634\n31912947\n29323892\n26441891\n22053761\n29982045",
    "results": null,
    "title": "Biocatalytic Production of a Nylon\u20056 Precursor from Caprolactone in Continuous Flow.",
    "xml": "<Element 'PubmedArticle' at 0x788506f7b830>"
}