{
    "abstract": "This study explores the effects of plant compounds on human papillomavirus (HPV)-induced W12 cervical precancer cells and bioelectric signaling. The aim is to identify effective phytochemicals, both individually and in combination, that can prevent and treat HPV infection and HPV associated cervical cancer. Phytochemicals were tested using growth inhibition, combination, gene expression, RT PCR, and molecular docking assays. W12 cells, derived from a cervical precancerous lesion, contain either episomal or integrated HPV16 DNA. Several compounds, including digoxin, tanshinone IIA, dihydromethysticin and carrageenan, as well as fractions of turmeric, ginger and pomegranate inhibited the growth of W12 precancer and cervical cancer cells. Curcumin and tanshinone IIA were the most active and relatively nontoxic compounds. RT-PCR analysis showed that tanshinone IIA activated the expression of p53, while repressing the expression of HPV16 E1, E2, E4, E6, and E7 viral transcripts in W12 (type 1 and 2) integrant cells. In addition, curcumin synergized with tanshinone IIA in HeLa cells. Molecular docking studies suggested tanshinone IIA and curcumin bind to the Na",
    "authors": [
        {
            "affiliation": "Center for Plants, People and Culture, The New York Botanical Garden, New York, NY 10458, USA.\nLehman College and the Graduate Center, City University of New York, New York, NY 10468, USA.\nDepartment of Rehabilitation and Regenerative Medicine, College of Physicians and Surgeons, Columbia University, HHSC-1518, 701 W. 168th Street, New York, NY 10032, USA.",
            "firstname": "Linda Saxe",
            "initials": "LS",
            "lastname": "Einbond"
        },
        {
            "affiliation": "Lehman College and the Graduate Center, City University of New York, New York, NY 10468, USA.",
            "firstname": "Jing",
            "initials": "J",
            "lastname": "Zhou"
        },
        {
            "affiliation": "Lehman College and the Graduate Center, City University of New York, New York, NY 10468, USA.",
            "firstname": "Kunhui",
            "initials": "K",
            "lastname": "Huang"
        },
        {
            "affiliation": "Staten Island University Hospital, Northwell Health, New York, NY 10305, USA.\nInnovene Therapeutics, Jersey City, NJ 07302, USA.",
            "firstname": "Mario R",
            "initials": "MR",
            "lastname": "Castellanos"
        },
        {
            "affiliation": "Lehman College and the Graduate Center, City University of New York, New York, NY 10468, USA.",
            "firstname": "Emeka",
            "initials": "E",
            "lastname": "Mbazor"
        },
        {
            "affiliation": "Center for Plants, People and Culture, The New York Botanical Garden, New York, NY 10458, USA.\nLehman College and the Graduate Center, City University of New York, New York, NY 10468, USA.",
            "firstname": "Michael",
            "initials": "M",
            "lastname": "Balick"
        },
        {
            "affiliation": "Department of Rehabilitation and Regenerative Medicine, College of Physicians and Surgeons, Columbia University, HHSC-1518, 701 W. 168th Street, New York, NY 10032, USA.",
            "firstname": "Hongbao",
            "initials": "H",
            "lastname": "Ma"
        },
        {
            "affiliation": "The Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY 11030, USA.",
            "firstname": "James A",
            "initials": "JA",
            "lastname": "DeVoti"
        },
        {
            "affiliation": "Lehman College and the Graduate Center, City University of New York, New York, NY 10468, USA.",
            "firstname": "Stephen",
            "initials": "S",
            "lastname": "Redenti"
        },
        {
            "affiliation": "Department of Rehabilitation and Regenerative Medicine, College of Physicians and Surgeons, Columbia University, HHSC-1518, 701 W. 168th Street, New York, NY 10032, USA.",
            "firstname": "Hsan-Au",
            "initials": "HA",
            "lastname": "Wu"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.3390/v17010055\n10.1002/biof.1344\n10.1002/pmic.200900178\n10.1002/pmic.201300274\n10.1016/j.canlet.2014.09.037\n10.3892/etm.2018.6182\n10.3390/molecules23092334\n10.18632/oncotarget.16648\n10.1038/s41416-020-01170-3\n10.1016/j.virol.2007.12.016\n10.1080/01635581.2018.1406123\n10.1371/journal.ppat.0020069\n10.1039/C5RA06002H\n10.1002/ijc.22897\n10.1055/s-2006-947225\n10.4161/cc.9.3.10556\n10.1016/j.fitote.2012.07.006\n10.3390/molecules26123672\n10.1002/jcb.26499\n10.1080/14786419.2014.951354\n10.3892/or.2016.5002\n10.1016/j.fct.2017.02.038\n10.1186/s40064-016-2639-x\n10.1021/jf073469n\n10.1091/mbc.e13-12-0708\n10.1016/j.ebiom.2021.103767\n10.1088/1478-3975/9/6/065002\n10.1016/j.ygyno.2012.12.005\n10.3851/IMP2612\n10.3390/molecules22040578\n10.3892/ol.2021.12919\n10.1016/j.biochi.2021.03.002\n10.1016/j.isci.2022.104963\n10.1126/sciadv.ade5393\n10.3390/molecules26071905\n10.3390/curroncol28030196\n10.1155/2012/716459\n10.1097/WNR.0000000000000559\n10.1016/j.jare.2014.12.007\n10.1016/j.cell.2021.02.034\n10.18632/oncotarget.8036\n10.2174/1381612823666170530105837\n10.1128/JVI.02296-13\n10.1371/journal.ppat.1006282\n10.1093/nar/gks545\n10.1038/srep06331\n10.1128/JVI.01582-19",
    "journal": "Viruses",
    "keywords": [
        "bioelectricity",
        "curcumin",
        "episomal",
        "molecular docking",
        "precancer",
        "tanshinone IIA"
    ],
    "methods": null,
    "publication_date": "2025-01-25",
    "pubmed_id": "39861845\n32162523\n28798073\n12460913\n27896883\n20049856\n24167031\n25304375\n29896225\n30213130\n28977833\n33257842\n18242658\n29227691\n16839203\n17621630\n16988912\n20081365\n22828666\n34208576\n29130514\n25141946\n27499229\n28249781\n27386358\n18412361\n25425556\n34933180\n23196890\n23234806\n23615820\n28430151\n34386080\n33713729\n36072548\n36763655\n33800655\n34201380\n22454677\n0\n26966780\n26644941\n33826908\n25525610\n26988909\n28554310\n24198405\n28306742\n22718976\n25208560\n31666385",
    "results": null,
    "title": "Plant Compounds Inhibit the Growth of W12 Cervical Precancer Cells Containing Episomal or Integrant HPV DNA; Tanshinone IIA Synergizes with Curcumin in Cervical Cancer Cells.",
    "xml": "<Element 'PubmedArticle' at 0x788504041f30>"
}