{
    "abstract": "Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV; Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR. The ejaculate was split into a control sample (C), a sample spiked with a 4 log TCID",
    "authors": [
        {
            "affiliation": "Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa. Electronic address: henry.annandale@up.ac.za.",
            "firstname": "C Henry",
            "initials": "CH",
            "lastname": "Annandale"
        },
        {
            "affiliation": "Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.",
            "firstname": "Mario P",
            "initials": "MP",
            "lastname": "Smuts"
        },
        {
            "affiliation": "Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.",
            "firstname": "Karen",
            "initials": "K",
            "lastname": "Ebersohn"
        },
        {
            "affiliation": "Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.",
            "firstname": "Lizette",
            "initials": "L",
            "lastname": "du Plessis"
        },
        {
            "affiliation": "Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa; School of Public Health, Medical and Veterinary Sciences, Discipline: Veterinary Science, James Cook University, Townsville, Australia.",
            "firstname": "Estelle H",
            "initials": "EH",
            "lastname": "Venter"
        },
        {
            "affiliation": "Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa; Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands.",
            "firstname": "Tom A E",
            "initials": "TAE",
            "lastname": "Stout"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2018 Elsevier B.V. All rights reserved.",
    "doi": "10.1016/j.anireprosci.2018.04.080",
    "journal": "Animal reproduction science",
    "keywords": [
        "Bull",
        "Lumpy skin disease virus",
        "Sanitation methods",
        "Semen"
    ],
    "methods": null,
    "publication_date": "2018-05-08",
    "pubmed_id": "29735243",
    "results": null,
    "title": "Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bull semen.",
    "xml": "<Element 'PubmedArticle' at 0x788502e9c400>"
}