{
    "abstract": "Goose astrovirus genotype 2 (GoAstV-2) is a novel and highly infectious pathogen characterized by symptoms of gout, swelling, and hemorrhage of the kidneys in 5-15-day-old goslings. The virus has caused severe economic losses for the goose husbandry industry. The capsid protein of GoAstV-2 is involved in viral packaging and immunogenicity, and it has served as a target antigen for diagnostic methods.\nIn this study, the N-terminus of the GoAstV-2 Cap protein (rCap) was successfully expressed and used to immunize BALB/c mice. A new monoclonal antibody (mAb) named 7B2 against the GoAstV-2 rCap protein was prepared. Immunofluorescence assay and Western blot analysis showed that mAb 7B2 had a strong binding affinity to the GoAstV-2 rCap protein. The results of epitope mapping demonstrated that the minimum linear epitope recognized by mAb 7B2 was located in the amino acids \nThese findings will be useful for the development of diagnostic kits for GoAstV-2 and will aid further research on the structure and function of the capsid protein.",
    "authors": [
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Haiqin",
            "initials": "H",
            "lastname": "Li"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Haoyu",
            "initials": "H",
            "lastname": "Huang"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Cui",
            "initials": "C",
            "lastname": "Lin"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Fanfan",
            "initials": "F",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Jia",
            "initials": "J",
            "lastname": "Tan"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Na",
            "initials": "N",
            "lastname": "Li"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Chengcheng",
            "initials": "C",
            "lastname": "Wu"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Jiangnan",
            "initials": "J",
            "lastname": "Huang"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Qipeng",
            "initials": "Q",
            "lastname": "Wei"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.",
            "firstname": "Siyi",
            "initials": "S",
            "lastname": "Wang"
        },
        {
            "affiliation": "Guangdong Provincial Biotechnology Research Institute, Guangzhou, Guangdong, 510633, China. 837895642@qq.com.",
            "firstname": "Yujun",
            "initials": "Y",
            "lastname": "Zhu"
        },
        {
            "affiliation": "Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China. zengyb2004@163.com.",
            "firstname": "Yanbing",
            "initials": "Y",
            "lastname": "Zeng"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2025. The Author(s).",
    "doi": "10.1186/s12917-025-04687-y",
    "journal": "BMC veterinary research",
    "keywords": [
        "B-cell linear epitope",
        "Cap protein",
        "Goose astrovirus genotypes 2",
        "Monoclonal antibody"
    ],
    "methods": null,
    "publication_date": "2025-04-23",
    "pubmed_id": "40264131\n29674726\n32402337\n29920970\n30366084\n36072471\n37167886\n29553061\n31669331\n29885801\n38909510\n28715976\n51251\n28316017\n30185786\n21069394\n21843659\n28106836\n15542679\n29883775\n34062934\n38587616\n36823127\n38836505\n22889825\n36649111\n35051673\n34391804\n32867970\n34438326\n31705830\n33518107\n12134004\n9343224\n34921327\n10644354\n34464644\n15802951\n20592088\n30355681\n28746401\n23276751\n37996850\n37065126\n22723097\n31384336\n33129191\n36996959",
    "results": "In this study, the N-terminus of the GoAstV-2 Cap protein (rCap) was successfully expressed and used to immunize BALB/c mice. A new monoclonal antibody (mAb) named 7B2 against the GoAstV-2 rCap protein was prepared. Immunofluorescence assay and Western blot analysis showed that mAb 7B2 had a strong binding affinity to the GoAstV-2 rCap protein. The results of epitope mapping demonstrated that the minimum linear epitope recognized by mAb 7B2 was located in the amino acids ",
    "title": "Identification of a novel linear B-cell epitope recognized by a monoclonal antibody against the capsid protein of goose astrovirus genotypes 2.",
    "xml": "<Element 'PubmedArticle' at 0x788505f46570>"
}