{
    "abstract": "This study aimed to investigate whether platelet-rich plasma (PRP) obtained from the blood of rats preconditioned with hyperbaric oxygen (HBOP) would enhance the biological activity of PRP and accelerate the healing process of femur fractures in a rat model.\nPRP was derived from blood samples of healthy rats subjected to either hyperbaric oxygen (hPRP) or normobaric air (nPRP). A closed femur fracture model was established in male Wistar rats, with treatments of hPRP or nPRP administered around the fracture site immediately post-fracture and on days 7, 14, 21, and 28. Growth factor concentrations in hPRP and nPRP were biochemically quantified. Bone healing was assessed weekly by X-ray, while histological and immunofluorescence analyses evaluated inflammatory status, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL) expression, and the presence of osteoblasts, osteoclasts, and osteocytes during healing. The effects of hPRP and nPRP on MC3T3-E1 preosteoblast migration and proliferation were also tested \nhPRP showed significantly higher concentrations of growth factors such as activin-A, brain-derived neurotrophic factor, nerve growth factor, Flt-3 Ligand, granulocyte-macrophage colony-stimulating factor, hepatocyte growth factor, and platelet-derived growth factor, compared to nPRP. \nHBOP enhances the biological activity of PRP and accelerates bone healing in a closed femur fracture model in rats. This study highlights the regenerative potential of PRP when preconditioned with hyperbaric oxygen for use in bone fracture therapy.\nPRP is widely used in treating bone defects and fractures, but its enhancement through HBOP remains underexplored. Our findings demonstrate that HBOP potentiates the biological activity of PRP, offering promising therapeutic potential for bone fracture healing.\nEnriching growth factors in PRP through HBOP could significantly improve tissue regeneration, especially in bone healing. The potential of hPRP in clinical applications is highly promising, particularly in orthopaedic surgery, trauma care, sports medicine, and managing bone healing in compromised patients.",
    "authors": [
        {
            "affiliation": "Department of Orthopedics, Chi Mei Medical Center, Tainan, Taiwan.",
            "firstname": "Wen-Shuo",
            "initials": "WS",
            "lastname": "Chang"
        },
        {
            "affiliation": "Department of Emergency Medicine, Chi Mei Medical Center, Tainan, Taiwan.\nSchool of Medicine, College of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan.\nDepartment of Emergency Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.",
            "firstname": "Chien-Cheng",
            "initials": "CC",
            "lastname": "Huang"
        },
        {
            "affiliation": "Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan.",
            "firstname": "Tzu-Hao",
            "initials": "TH",
            "lastname": "Chen"
        },
        {
            "affiliation": "Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan.",
            "firstname": "Ssu-Han",
            "initials": "SH",
            "lastname": "Chao"
        },
        {
            "affiliation": "Department of Medicine, MacKay Medical College, New Taipei City, Taiwan.",
            "firstname": "Cheng-Hsien",
            "initials": "CH",
            "lastname": "Lin"
        },
        {
            "affiliation": "Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan.",
            "firstname": "Ching-Ping",
            "initials": "CP",
            "lastname": "Chang"
        },
        {
            "affiliation": "Department of Orthopedics, Chi Mei Medical Center, Tainan, Taiwan.",
            "firstname": "Chi-Sheng",
            "initials": "CS",
            "lastname": "Chien"
        }
    ],
    "conclusions": null,
    "copyrights": "\u00a9 2024 The Authors.",
    "doi": "10.1016/j.jot.2024.10.016\n10.1016/j.biopha.2020.109991\n10.1016/j.celrep.2020.108052\n10.1080/14712598.2017.1259409\n10.1186/s12891-022-06073-3\n10.3727/096368912x653183\n10.3109/09537104.2012.684730\n10.7759/cureus.54122\n10.1302/0301-620x.91b8.22546\n10.1111/jcpe.13546\n10.1016/j.joms.2017.01.022\n10.1016/j.jcms.2019.01.012\n10.1186/1749-799x-5-91\n10.5606/ehc.2020.72592\n10.1002/jor.22443\n10.3892/etm.2018.6776\n10.2147/dddt.S80778\n10.1007/s00167-013-2734-2\n10.1016/j.injury.2017.04.040\n10.1016/j.bonr.2020.100250\n10.1016/j.mce.2024.112175\n10.3967/bes2015.006\n10.1007/s11999-009-1004-6\n10.1016/j.bbrc.2019.04.093\n10.1016/j.injury.2016.09.039\n10.1111/j.1600-0501.2005.01145.x\n10.1038/s41598-019-43798-z\n10.7554/eLife.89822\n10.1002/advs.202003390\n10.1007/s11914-024-00869-z\n10.1186/s13287-023-03547-6\n10.3390/ijms242216175\n10.1096/fj.202301508RRR\n10.3389/fendo.2024.1386556\n10.7554/eLife.95083\n10.1002/jor.22805\n10.3390/ijms21041452\n10.22203/ecm.v015a05\n10.1016/j.bonr.2017.03.002\n10.3390/antiox11112302\n10.1056/NEJMoa035003\n10.1161/01.ATV.0000163262.83456.6d\n10.1016/j.jcrc.2010.09.001\n10.1186/2047-783x-19-12\n10.3109/03008201003746679\n10.1186/1471-2474-15-56\n10.1186/s13075-019-1830-1\n10.1016/j.arthro.2019.04.010\n10.2147/dddt.S356858\n10.1016/j.dsx.2017.09.007\n10.1177/0363546517743746\n10.5051/jpis.2300660033\n10.34172/jlms.2021.60\n10.1097/md.0000000000035854\n10.3389/fphys.2020.553198\n10.3389/fpain.2023.1216190\n10.1186/s12929-023-00972-w",
    "journal": "Journal of orthopaedic translation",
    "keywords": [
        "Bone healing",
        "Growth factor",
        "Hyperbaric oxygen",
        "Osteogenesis",
        "Platelet-rich plasma"
    ],
    "methods": null,
    "publication_date": "2025-02-04",
    "pubmed_id": "39902098\n32084699\n32905775\n27845852\n36517775\n22863146\n22647081\n38487114\n19651823\n23648197\n34517437\n28236425\n30711470\n21134254\n32584723\n23878004\n30542406\n25897207\n24170188\n28456368\n32090156\n38341020\n25566863\n22773585\n19653054\n31047639\n27817884\n16117770\n31165745\n38079220\n33854888\n38616228\n37936199\n38003376\n38588175\n38757000\n38591777\n25640997\n32093391\n18382990\n28377988\n36421487\n14573731\n15790935\n21036525\n24588870\n20497028\n24568330\n30704538\n31604507\n35300222\n29050916\n29268037\n37336521\n35155145\n38013292\n33041857\n37396144\n37704991",
    "results": "hPRP showed significantly higher concentrations of growth factors such as activin-A, brain-derived neurotrophic factor, nerve growth factor, Flt-3 Ligand, granulocyte-macrophage colony-stimulating factor, hepatocyte growth factor, and platelet-derived growth factor, compared to nPRP. ",
    "title": "Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing.",
    "xml": "<Element 'PubmedArticle' at 0x7885059b5670>"
}