{
    "abstract": "What are the effects of pipette- versus laser-assisted artificial blastocyst collapse (ABC) on the morphokinetics of warmed blastocyst re-expansion, and what is the potential effect on treatment outcomes?\nSurplus blastocysts were extracted from 203 patients. These were divided into three groups: study group A, artificial collapsed by the aspiration of blastocoel fluid with a pipette; study group B, trophectoderm opened with a laser pulse; control group, no manipulation before vitrification was performed. During the 5-year study period, 257 associated single-warm blastocyst transfers were scheduled. The start and duration of the re-expansion process before transfer were annotated. Pregnancy and live birth data were also collected for the transfers.\nThe overall blastocyst survival rate was 96.9%, with no effect observed as a result of the two ABC methods. The re-expansion of blastocysts in study group B was initiated significantly sooner after warming (0.50 \u00b1 0.37 h) than in group A (0.79 \u00b1 0.56 h) or the control group (1.22 \u00b1 1.00 h). The duration of the re-expansion process was significantly reduced in study groups A (P\u202f=\u202f0.021) and B (P\u202f=\u202f0.004) compared with the control group. The embryos of participants who achieved a live birth had a significantly (P < 0.001) faster start of re-expansion (0.60 \u00b1 0.42 h) than the embryos in those who did not produce an ongoing pregnancy (1.05 \u00b1 0.92 h).\nLaser-treated blastocysts exhibited substantially shorter re-expansion times. Because faster re-expansion of the blastocyst is associated with positive treatment outcomes, the laser technique should be prioritized over the pipetting technique if ABC is considered.",
    "authors": [
        {
            "affiliation": "Ginefiv Barcelona, GeneraLife, Barcelona, Spain; Departament de Medicina i Cirurgia Animals, Universitat Aut\u00f2noma de Barcelona, Barcelona, Spain.",
            "firstname": "Iris",
            "initials": "I",
            "lastname": "Mart\u00ednez-Rodero"
        },
        {
            "affiliation": "Department of Reproductive Medicine and Gynaecological Endocrinology, University Medical Centre Maribor, Maribor, Slovenia.",
            "firstname": "Borut",
            "initials": "B",
            "lastname": "Kova\u010di\u010d"
        },
        {
            "affiliation": "Department of Gynaecology, Obstetrics, and Gynaecological Endocrinology, Kepler University Hospital, Linz, Austria.",
            "firstname": "Omar",
            "initials": "O",
            "lastname": "Shebl"
        },
        {
            "affiliation": "Department of Gynaecology, Obstetrics, and Gynaecological Endocrinology, Kepler University Hospital, Linz, Austria.",
            "firstname": "Sabine",
            "initials": "S",
            "lastname": "Enengl"
        },
        {
            "affiliation": "Department of Gynaecology, Obstetrics, and Gynaecological Endocrinology, Kepler University Hospital, Linz, Austria.",
            "firstname": "Julia",
            "initials": "J",
            "lastname": "Lastinger"
        },
        {
            "affiliation": "Department of Gynaecology, Obstetrics, and Gynaecological Endocrinology, Kepler University Hospital, Linz, Austria. Electronic address: Thomas.ebner@kepleruniklinikum.at.",
            "firstname": "Thomas",
            "initials": "T",
            "lastname": "Ebner"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2024 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.",
    "doi": "10.1016/j.rbmo.2024.104476",
    "journal": "Reproductive biomedicine online",
    "keywords": [
        "Artificial collapse",
        "Blastocoelic fluid",
        "Implantation rate",
        "Live birth rate",
        "Time-lapse",
        "Vitrification"
    ],
    "methods": null,
    "publication_date": "2025-01-02",
    "pubmed_id": "39742815",
    "results": "The overall blastocyst survival rate was 96.9%, with no effect observed as a result of the two ABC methods. The re-expansion of blastocysts in study group B was initiated significantly sooner after warming (0.50 \u00b1 0.37 h) than in group A (0.79 \u00b1 0.56 h) or the control group (1.22 \u00b1 1.00 h). The duration of the re-expansion process was significantly reduced in study groups A (P\u202f=\u202f0.021) and B (P\u202f=\u202f0.004) compared with the control group. The embryos of participants who achieved a live birth had a significantly (P < 0.001) faster start of re-expansion (0.60 \u00b1 0.42 h) than the embryos in those who did not produce an ongoing pregnancy (1.05 \u00b1 0.92 h).",
    "title": "The effect of pipette- and laser-induced blastocyst collapse before vitrification on their re-expansion and clinical outcome after warming.",
    "xml": "<Element 'PubmedArticle' at 0x788501f8ce00>"
}