{
    "abstract": "Several similarities have been found between shear stress-induced erythrocyte damage and physiological aging of erythrocytes in terms of elevated mechanical fragility, increased erythrocyte aggregation, and decreased membrane surface charge. Accordingly, we hypothesized that blood pump circulation, which generates shear stress, would accelerate erythrocyte aging, manifesting as oxidation. Therefore, the purpose of this study was to investigate the effect of blood pump circulation on erythrocyte oxidation. Fresh porcine blood was acquired from a slaughterhouse and anticoagulated with sodium citrate. About 500\u2009mL of anticoagulated whole blood was circulated for 180\u2009min in an in vitro test circuit comprising a BP-80 blood pump with a pump speed and a pump pressure head of 100-120\u2009mmHg. A blood sample was taken at the start of the circulation and 180\u2009min afterward. The hemolysis level and oxidation amount of the erythrocyte membrane were analyzed and compared between samples. Hemolysis increased with the prolongation of shear exposure inside the pump circuit. After 180\u2009min of blood pumping in circuit, the oxidation level of the erythrocyte membrane showed an increase of 0.1\u2009nmol/mg protein. Moreover, the membrane oxidation levels of sheared erythrocytes were greater than those of control erythrocytes. These results suggest that blood pump circulation accelerates erythrocyte aging and give us a greater understanding of the effects of blood pump perfusion.",
    "authors": [
        {
            "affiliation": "Functional Control Systems, Graduate School of Engineering and Science, Shibaura Institute of Technology-Omiya Campus, Saitama, Saitama Prefecture, Japan.",
            "firstname": "Masataka",
            "initials": "M",
            "lastname": "Inoue"
        },
        {
            "affiliation": "Systems Engineering and Science, Graduate School of Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.",
            "firstname": "Yuki",
            "initials": "Y",
            "lastname": "Udono"
        },
        {
            "affiliation": "Division of Biochemistry, Department of Pathological and Therapeutic Science, Faculty of Medicine, Tottori University, Tottori, Japan.\nMolecular Cell Biology Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.",
            "firstname": "Yugo",
            "initials": "Y",
            "lastname": "Kato"
        },
        {
            "affiliation": "Molecular Cell Biology Laboratory, Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.",
            "firstname": "Koji",
            "initials": "K",
            "lastname": "Fukui"
        },
        {
            "affiliation": "Functional Control Systems, Graduate School of Engineering and Science, Shibaura Institute of Technology-Omiya Campus, Saitama, Saitama Prefecture, Japan.\nSystems Engineering and Science, Graduate School of Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.",
            "firstname": "Nobuo",
            "initials": "N",
            "lastname": "Watanabe"
        }
    ],
    "conclusions": null,
    "copyrights": null,
    "doi": "10.1177/03913988241230942",
    "journal": "The International journal of artificial organs",
    "keywords": [
        "LVAD",
        "Shear stress",
        "erythrocyte",
        "hemolysis",
        "membrane oxidation"
    ],
    "methods": null,
    "publication_date": "2024-03-01",
    "pubmed_id": "38425132\n14000509\n25676371\n25233911\n7492978\n16056254\n27354532\n28222499\n22445181\n23212552\n25010916\n19920051\n11063882\n8532063\n10569637\n3007097\n4056060\n2212862\n8573845\n28396088\n31857631\n21810113\n17312862\n13646603\n22882442\n30126305\n34876652\n29995802\n33841180\n9299949\n5016112\n12780506\n28868706\n28543744\n17470986\n18243141\n24616707",
    "results": null,
    "title": "Evaluation of erythrocyte membrane oxidation due to their exposure to shear flow generated by extracorporeal blood pump.",
    "xml": "<Element 'PubmedArticle' at 0x788506ca6610>"
}