{
    "abstract": "Components of the plasminogen/plasmin system, known to be present in the oocyte, play a key role in maturation and fertilization. The objective of this study was to examine the effect of plasminogen activation and plasmin inhibition by exogenous supplementation of the IVF medium with streptokinase (SK) or \u025b-aminocaproic acid (\u03b5-ACA), respectively, on fertilization parameters and preimplantation embryo development. After in vitro maturation, bovine cumulus-oocyte complexes (COCs) were inseminated in the presence of SK or \u03b5-ACA. The addition of SK to the IVF medium facilitated the adhesion of the spermatozoa to the zona pellucida without affecting the percentages of monospermy. Cleavage rates and blastocyst yield were similar between the SK and Control groups while they were lower with the \u03b5-ACA treatment. Additionally, we found that the expression levels of embryo quality-related genes (SDHA and DNMT3A) could be modified in blastocysts by the addition of SK or \u03b5-ACA during IVF. The results obtained indicate that supplementation of the IVF medium with SK did not greatly alter the embryonic developmental parameters related to embryo quality in blastocysts. Moreover, we noticed that \u03b5-ACA treatment compromises the success of in vitro embryo development, thus highlighting the importance of the plasminogen/plasmin activity during the early stages of embryogenesis in bovine.",
    "authors": [
        {
            "affiliation": "Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO), CONICET-UNT, and Instituto de Biolog\u00eda 'Dr. Francisco D. Barbieri', Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, UNT, Chacabuco 461 (4000), San Miguel de Tucum\u00e1n, Tucum\u00e1n, Argentina.",
            "firstname": "Gabriela",
            "initials": "G",
            "lastname": "Rizo"
        },
        {
            "affiliation": "Laboratorio de Biolog\u00eda Molecular, Facultad de Ciencias Agrarias y Veterinarias, Universidad Cat\u00f3lica de Salta (UCASAL) and Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Campo Casta\u00f1ares (4400), Salta, Argentina.",
            "firstname": "Antonio Daniel",
            "initials": "AD",
            "lastname": "Barrera"
        },
        {
            "affiliation": "Laboratorio de Biolog\u00eda Molecular, Facultad de Ciencias Agrarias y Veterinarias, Universidad Cat\u00f3lica de Salta (UCASAL) and Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Campo Casta\u00f1ares (4400), Salta, Argentina.",
            "firstname": "Elina Vanesa",
            "initials": "EV",
            "lastname": "Garc\u00eda"
        },
        {
            "affiliation": "Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO), CONICET-UNT, and Instituto de Biolog\u00eda 'Dr. Francisco D. Barbieri', Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia, UNT, Chacabuco 461 (4000), San Miguel de Tucum\u00e1n, Tucum\u00e1n, Argentina. Electronic address: eugenia.roldanolarte@fbqf.unt.edu.ar.",
            "firstname": "Mariela",
            "initials": "M",
            "lastname": "Rold\u00e1n-Olarte"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.",
    "doi": "10.1016/j.repbio.2023.100844",
    "journal": "Reproductive biology",
    "keywords": [
        "IVF",
        "Plasminogen activation",
        "Preimplantation embryo development",
        "Streptokinase",
        "\u025b-aminocaproic acid"
    ],
    "methods": null,
    "publication_date": "2024-01-02",
    "pubmed_id": "38160587",
    "results": null,
    "title": "Plasminogen activation and plasmin inhibition during in vitro fertilization in bovine: implications for fertilization parameters and early embryo development.",
    "xml": "<Element 'PubmedArticle' at 0x7885052a8310>"
}