{
    "abstract": "As a classical regulating formula, Guominkang (GMK) has been extensively employed in clinical practice to treat the allergic asthma (AA) and alleviate allergy symptoms, however, the underlying mechanism remains elusive. The aim of this study was to explored the mechanism of action through which GMK combats AA.\nPotential target genes for the compounds were identified from the database and subjected to functional enrichment analysis. Subsequently, a protein-protein interaction (PPI) network was constructed in order to screen the core target and confirmed by molecular docking. An asthma model was further developed in mice and airway hyperresponsiveness and lung pathological changes were observed following drug administration. The expression of PI3K and AKT proteins in lung tissues was then detected by Western blotting. Subsequently, the GSE104468 data were normalised and visualised using the R language, compared to the PI3K-Akt pathway gene set to identify overlapping genes, constructed a PPI network and analysed correlations between genes.\n267 compounds and 475 disease-relevant GMK targets have been obtained, primarily in the areas of chemokine binding, drug binding, and PI3K-Akt pathway modulation. Molecular docking simulations revealed that predicted targets (PI3K, TNF, IL6, AKT1, SRC, TP53, and STAT3) could be closely bonded with component of GMK. According to in vivo experiments, GMK could reduce mucus obstruction and airway inflammation (\nBased on our findings, GMK potentially acts via the PI3K/Akt pathway and alleviates allergic symptoms in AA.",
    "authors": [
        {
            "affiliation": "National Institute of TCM Body Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.\nCollege of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.",
            "firstname": "Honglei",
            "initials": "H",
            "lastname": "Zhang"
        },
        {
            "affiliation": "National Institute of TCM Body Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.\nCollege of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.\nDalian Women and Children's Medical Group, Dalian, 116000, People's Republic of China.",
            "firstname": "Haiyun",
            "initials": "H",
            "lastname": "Zhang"
        },
        {
            "affiliation": "Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, People's Republic of China.",
            "firstname": "Lei",
            "initials": "L",
            "lastname": "Wang"
        },
        {
            "affiliation": "National Institute of TCM Body Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.\nCollege of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.",
            "firstname": "Yihang",
            "initials": "Y",
            "lastname": "Zhang"
        },
        {
            "affiliation": "National Institute of TCM Body Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.\nCollege of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.",
            "firstname": "Linhan",
            "initials": "L",
            "lastname": "Hu"
        },
        {
            "affiliation": "National Institute of TCM Body Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.\nCollege of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.",
            "firstname": "Juntong",
            "initials": "J",
            "lastname": "Liu"
        },
        {
            "affiliation": "National Institute of TCM Body Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.",
            "firstname": "Yumei",
            "initials": "Y",
            "lastname": "Zhou"
        },
        {
            "affiliation": "National Institute of TCM Body Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.",
            "firstname": "Ji",
            "initials": "J",
            "lastname": "Wang"
        }
    ],
    "conclusions": "Based on our findings, GMK potentially acts via the PI3K/Akt pathway and alleviates allergic symptoms in AA.",
    "copyrights": "\u00a9 2025 Zhang et al.",
    "doi": "10.2147/JIR.S484251\n10.1183/09031936.00138707\n10.1016/j.jaip.2020.10.044\n10.1038/s41577-022-00735-y\n10.3238/arztebl.2020.0434\n10.1097/md.0000000000036892\n10.3389/fphar.2022.978421\n10.1007/s10753-021-01466-3\n10.1111/resp.12880\n10.1016/j.jep.2021.113894\n10.1016/j.jep.2023.117565\n10.2202/1544-6115.1027\n10.1186/1758-2946-6-13\n10.1093/nar/gkaa1063\n10.1093/nar/gkaa971\n10.1038/srep42717\n10.1093/nar/gkz382\n10.1093/nar/gkaa1100\n10.1093/nar/gku1205\n10.1016/j.csbj.2021.05.015\n10.1002/cpbi.5\n10.1093/nar/gkx1037\n10.1101/gr.1239303\n10.1093/nar/gkaa1074\n10.1038/s41598-020-79235-9\n10.1016/j.biosystems.2014.11.005\n10.1691/ph.2019.9080\n10.1038/s41392-023-01344-4\n10.1016/j.jaip.2019.11.008\n10.3389/fimmu.2021.637948\n10.1007/s00281-016-0601-1\n10.1038/srep05865\n10.1038/s41374-021-00618-1\n10.3390/molecules26113117\n10.1016/s0140-6736(17)33311-1\n10.1248/bpb.32.444\n10.1016/j.bcp.2020.114401\n10.3390/molecules27207028\n10.1016/j.jaci.2021.11.026\n10.1038/s41598-017-09349-0\n10.1007/bf03262206\n10.3892/ijmm.2020.4622\n10.1016/j.jaci.2021.12.783\n10.1007/s10753-017-0581-x\n10.1016/j.intimp.2021.108266",
    "journal": "Journal of inflammation research",
    "keywords": [
        "PI3K/Akt signaling pathway",
        "allergic diseases",
        "network pharmacology",
        "traditional Chinese medicine"
    ],
    "methods": null,
    "publication_date": "2025-03-03",
    "pubmed_id": "40026302\n18166595\n33161172\n35650271\n32885783\n38363941\n36330091\n33860870\n27556731\n33516930\n38081397\n16646809\n24735618\n33264402\n33151290\n28256516\n31106366\n33237286\n25428349\n34136095\n27322403\n29126136\n14597658\n33237311\n33446695\n25451770\n31685084\n36964157\n32037107\n33643321\n27858144\n25109392\n34108631\n34071080\n34085124\n29273246\n19252293\n33387483\n36296620\n34974067\n28855674\n22519966\n32626920\n34974065\n28493079\n34678694",
    "results": "267 compounds and 475 disease-relevant GMK targets have been obtained, primarily in the areas of chemokine binding, drug binding, and PI3K-Akt pathway modulation. Molecular docking simulations revealed that predicted targets (PI3K, TNF, IL6, AKT1, SRC, TP53, and STAT3) could be closely bonded with component of GMK. According to in vivo experiments, GMK could reduce mucus obstruction and airway inflammation (",
    "title": "Bioinformatics and Network Pharmacology Identify the Therapeutic Role of Guominkang in Allergic Asthma by Inhibiting PI3K/Akt Signaling.",
    "xml": "<Element 'PubmedArticle' at 0x788506206890>"
}