{
    "abstract": "To achieve system-level insights into proteome organization, regulation, and function, we developed an approach to generate complex cell pools with endogenously tagged proteins amenable to high-throughput visualization and perturbation. Pooled imaging coupled to in situ barcode sequencing identified the subcellular localization of each HaloTag-tagged protein, and subsequent ligand-induced misfolding of the library followed by single-cell RNA sequencing revealed responses to spatially restricted protein misfolding. These datasets characterized protein quality control responses in previously uninterrogated cellular compartments, and cross-compartment analyses revealed mutually exclusive rather than collaborative responses, whereby the heat shock response (HSR) is induced in some compartments and repressed in others where autophagy genes are induced. We further assign protein quality control functions to previously uncharacterized genes based on shared transcriptional responses to protein misfolding across cellular compartments. Altogether, we present an efficient method for large-scale studies of proteome dynamics, function, and homeostasis.",
    "authors": [
        {
            "affiliation": "Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.",
            "firstname": "Stephanie E",
            "initials": "SE",
            "lastname": "Sansbury"
        },
        {
            "affiliation": "Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: yevgeniy.serebrenik@rutgers.edu.",
            "firstname": "Yevgeniy V",
            "initials": "YV",
            "lastname": "Serebrenik"
        },
        {
            "affiliation": "Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.",
            "firstname": "Tomer",
            "initials": "T",
            "lastname": "Lapidot"
        },
        {
            "affiliation": "Center for Single Cell Biology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.",
            "firstname": "David G",
            "initials": "DG",
            "lastname": "Smith"
        },
        {
            "affiliation": "Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.",
            "firstname": "George M",
            "initials": "GM",
            "lastname": "Burslem"
        },
        {
            "affiliation": "Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: shalemo@upenn.edu.",
            "firstname": "Ophir",
            "initials": "O",
            "lastname": "Shalem"
        }
    ],
    "conclusions": null,
    "copyrights": "Copyright \u00a9 2025 Elsevier Inc. All rights reserved.",
    "doi": "10.1016/j.molcel.2025.04.002",
    "journal": "Molecular cell",
    "keywords": [
        "hydrophobic targeting",
        "in situ sequencing",
        "pooled tagging",
        "protein localization",
        "protein misfolding",
        "proteostasis"
    ],
    "methods": null,
    "publication_date": "2025-04-25",
    "pubmed_id": "40273915",
    "results": null,
    "title": "Pooled tagging and hydrophobic targeting of endogenous proteins for unbiased mapping of unfolded protein responses.",
    "xml": "<Element 'PubmedArticle' at 0x788505f80090>"
}